Human FoxC1 Antibody

Catalog #: MAB6329 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6329
MAB6329-SP

Detection of Human FoxC1 by Western Blot.

1 Image

All FoxC1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxC1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FoxC1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 625905

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FoxC1
Gln208-Gln322
Accession # Q12948

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human FoxC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, NCI-H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human FoxC1 Monoclonal Antibody (Catalog # MAB6329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxC1

Human FoxC1 (forkhead box C1), also called FKHL7 (forkhead-like 7) or FREAC3 (forkhead-related activator 3) is a 553 amino acid (aa) intracellular phosphoprotein that belongs to a large family of nuclear transcription factors that share a common forkhead/winged helix DNA binding domain. FoxC1 is essential for formation of mesodermal tissues; mutations underlie Axenfeld-Rieger malformations of the eye. The human FoxC1 sequence includes a DNA binding domain at aa 77‑168 and an inhibitory domain between aa 268‑245. Phosphorylation within this inhibitory region causes a protein gel mobility shift. Human FoxC1 shares 92% and 91% aa identity with mouse and rat FoxC1, respectively.

Long Name

Forkhead Box C1

Entrez Gene IDs

2296 (Human); 17300 (Mouse)

Alternate Names

ARA; FKHL7; FKHL7RIEG3; forkhead box C1; forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead/winged helix-like transcription factor 7; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FoxC1; FREAC3; FREAC-3; FREAC3IRID1; IGDA; IHG1; IRID1; myeloid factor-delta; RIEG3