Human GCHFR Antibody

Catalog #: MAB7918 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7918
MAB7918-SP

Detection of Human and Mouse GCHFR by Western Blot.

2 Images

All GCHFR Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GCHFR Antibody Summary

Species Reactivity

Human

Specificity

Detects human GCHFR in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 846011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GCHFR
Met1-Glu84
Accession # P30047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human and Mouse GCHFR by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Bowes human melanoma cell line, and XB2 mouse teratoma keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GCHFR at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human GCHFR by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for GCHFR at approximately 11 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GCHFR Monoclonal Antibody (Catalog # MAB7918). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GCHFR

GCHFR (GTP cyclohydrolase I feedback receptor), also called GFRP, is an 84 amino acid enzyme inhibitor that binds to and regulates the activity of GTP cyclohydrolase I. GTP cyclohydrolase I is the rate-limiting enzyme in biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthases and aromatic hydrolases. GCHFR is found as a 20 kDa homodimer in the nucleus and cytoplasm of multiple cell types, including endothelial cells, keratinocytes and melanocytes. Human GCHFR shares 93% amino acid sequence identity with mouse and rat GCHFR, which in turn share 100% identity with each other.

Long Name

GTP Cyclohydrolase I Feedback Regulator

Entrez Gene IDs

2644 (Human); 320415 (Mouse); 171128 (Rat)

Alternate Names

GCHFR; GFRP; GFRPP35; GTP cyclohydrolase I feedback regulator; GTP cyclohydrolase I feedback regulatory proteinGTP cyclohydrolase 1 feedback regulatory protein; HsT16933; MGC138467; MGC138469; p35