Human Gremlin Antibody

Catalog #: MAB5190 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5190-100
MAB5190-SP

1 Image

All Gremlin Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Gremlin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Gremlin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 955320

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Gremlin
Met1-Asp184
Accession # O60565

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB51901).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Gremlin DuoSet ELISA Kit (Catalog # DY5190-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human Gremlin ELISA Standard Curve. Recombinant Human Gremlin protein was serially diluted 2-fold and captured by Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB51901) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Gremlin Monoclonal Antibody (Catalog # MAB5190) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gremlin

Gremlin was identified in a Xenopus expression-cloning screen as a dorsalizing factor that can induce a secondary axis. A rat homolog, called Drm, was identified as a cDNA that was downregulated in v-mos transfected cells. Gremlin/Drm belongs to the DAN family of secreted glycoproteins that are BMP antagonists. Other members of the family include: Cerberus, Dante, PRDC, Caronte, and DAN. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. In vitro, Gremlin/Drm binds BMP-4 and BMP-2 indicating that it might interfere with BMP signaling. Gremlin/Drm acts as a BMP-2/4 antagonist in a variety of tissues and developmental processes including: Xenopus animal cap explants, chick limb bud outgrowth and chondrogenesis, murine lung branching morphogenesis, and osteogenic differentiation of mouse myoblasts and bone marrow stromal cells. In addition, expression of Gremlin/Drm has been shown to be downregulated in a wide range of human cancer cell lines. Mouse, human, chick and Xenopus homologs of Gremlin share over 80% amino acid identity. It is likely that various DAN family members and other BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands.

References

Hsu, D.R. et al. (1998) Mol. Cell 1:673.
Merino, R. et al. (1999) Development 126:5515.
Shi, W. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 280:L1030.
Topol, L.Z. et al. (2000) J. Biol. Chem. 275:8785.

Entrez Gene IDs

26585 (Human); 23892 (Mouse)

Alternate Names

Cell proliferation-inducing gene 2 protein; CKTSF1B1gremlin 1, cysteine knot superfamily, homolog; Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); DAN domain family member 2; DAND2; DAND2GREMLIN; Down-regulated in Mos-transformed cells protein; DRM; DRMMGC126660; GREM1; gremlin 1; gremlin 1-like protein; Gremlin; gremlin-1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; proliferation-inducing gene 2