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Human IL-1 alpha/IL-1F1 DuoSet ELISA

Catalog #: DY200
31 Citations 5 Reviews Datasheet / COA / SDS

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DY200 has been discontinued.
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Human IL-1 alpha / IL-1F1 ELISA Standard Curve
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Citations (31)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human IL-1 alpha/IL-1F1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-1alpha/IL-1F1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human IL-1 alpha / IL-1F1 ELISA Standard Curve View Larger

Human IL-1 alpha / IL-1F1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 alpha/IL-1F1

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names:
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human IL-1 alpha/IL-1F1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Human TH17 cells engage gasdermin E pores to release IL-1alpha on NLRP3 inflammasome activation
    Authors: YY Chao, A Puhach, D Frieser, M Arunkumar, L Lehner, T Seeholzer, A Garcia-Lop, M van der Wa, S Fibi-Smeta, A Dietschman, T Sommermann, T ?ikovi?, L Taher, MS Gresnigt, SJ Vastert, F van Wijk, G Panagiotou, D Krappmann, O Gro beta, CE Zielinski
    Nature Immunology, 2023-01-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. A whole genome CRISPR screen identifies AHR loss as a mechanism of PARP7 inhibitor resistance
    Authors: H Chen, ME Diolaiti, PC O'Leary, A Rojc, NJ Krogan, M Kim, A Ashworth
    Molecular Cancer Therapeutics, 2022-07-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. A prospective cohort study providing insights for markers of adverse pregnancy outcome in older mothers
    Authors: SC Lean, RL Jones, SA Roberts, AEP Heazell
    BMC pregnancy and childbirth, 2021-10-20;21(1):706.
    Species: Human
    Sample Types: Serum
  4. Skin Sensitization Evaluation of Carbon-Based Graphene Nanoplatelets
    Authors: SH Kim, SH Hong, JH Lee, DH Lee, K Jung, JY Yang, HS Shin, J Lee, J Jeong, JH Oh
    Toxics, 2021-03-17;9(3):.
    Species: Human
    Sample Types: cell culture supernatant
  5. Synthesis of Retinol-Loaded Lipid Nanocarrier via Vacuum Emulsification to Improve Topical Skin Delivery
    Authors: SH Jun, H Kim, H Lee, JE Song, SG Park, NG Kang
    Polymers, 2021-03-08;13(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation
    Authors: K Bernau, JP Leet, H Floerke, EM Bruhn, AL Noll, IS McDermott, S Esnault, NN Jarjour, N Sandbo
    Cells, 2021-03-02;10(3):.
    Species: Human
    Sample Types: BALF
  7. Acid-electrolyzed functional water-induces Interleukin-1&alpha release from Intracellular Storage Sites in Oral Squamous Cell Carcinoma
    Authors: T Takemoto, R Kaetsu, M Hanayama, Y Ishiyama, M Sadamura, K Nishio, M Tsunoda, M Asano, M Motoyoshi
    International Journal of Medical Sciences, 2021-02-18;18(8):1746-1752.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. ROCK inhibition modulates the senescence-associated secretory phenotype (SASP) in oral keratinocytes
    Authors: S Niklander, D Bandaru, DW Lambert, KD Hunter
    FEBS Open Bio, 2020-11-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. The Delivery of &alpha1-Antitrypsin Therapy Through Transepidermal Route: Worthwhile to Explore
    Authors: S Tumpara, B Martinez-D, G Gomez-Mari, B Liu, DS DeLuca, E Korenbaum, D Jonigk, F Jugert, FM Wurm, MJ Wurm, T Welte, S Janciauski
    Front Pharmacol, 2020-07-03;11(0):983.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Cytotoxic and Anti-Inflammatory Effects of Ent-Kaurane Derivatives Isolated from the Alpine Plant Sideritis hyssopifolia
    Authors: A Aimond, K Calabro, C Audoin, E Olivier, M Dutot, P Buron, P Rat, O Laprévote, S Prado, E Roulland, OP Thomas, G Genta-Jouv
    Molecules, 2020-01-29;25(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Ouabain signaling in oral squamous cell carcinoma cells
    Authors: S Saito, M Ohtsu, M Asano, T Ishigami
    J Oral Sci, 2019-09-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Inflammasome-Driven Interleukin-1α and�Interleukin-1? Production in Atherosclerotic Plaques Relates to Hyperlipidemia and Plaque Complexity
    Authors: X Jiang, F Wang, Y Wang, A Gisterå, J Roy, G Paulsson-B, U Hedin, A Lerman, GK Hansson, J Herrmann, ZQ Yan
    JACC Basic Transl Sci, 2019-06-24;4(3):304-317.
    Species: Human
    Sample Types: Tissue Culture Supernates
  13. Cytokine Diversity in Human Peripheral Blood Eosinophils: Profound Variability of IL-16
    Authors: M Ma, CM Percopo, DE Sturdevant, AC Sek, HD Komarow, HF Rosenberg
    J. Immunol., 2019-06-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  14. Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist
    Authors: S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett
    Stem Cells, 2019-05-13;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells
    Authors: R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa
    Oncol Lett, 2019-01-29;17(3):3555-3561.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
    Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
    Cell Rep, 2018-12-26;25(13):3706-3720.e8.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Modulation of salivary cytokines in response to alcohol, tobacco and caffeine consumption: a pilot study
    Authors: CC Sheth, RM López-Pedr, MDM Jovani-San, R González-M, V Veses
    Sci Rep, 2018-11-12;8(1):16687.
    Species: Human
    Sample Types: Saliva
  18. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
    Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
    Int J Med Sci, 2018-09-07;15(12):1365-1372.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. The Effects of Varying Degree of MWCNT Carboxylation on Bioactivity in Various In Vivo and In Vitro Exposure Models
    Authors: RF Hamilton, Z Wu, S Mitra, A Holian
    Int J Mol Sci, 2018-01-25;19(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11
    Authors: B Lagrange, S Benaoudia, P Wallet, F Magnotti, A Provost, F Michal, A Martin, F Di Lorenzo, BF Py, A Molinaro, T Henry
    Nat Commun, 2018-01-16;9(1):242.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017-08-22;18(1):159.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017-07-13;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  23. TB lymphadenitis is associated with enhanced baseline and antigen - specific induction of Type 1 and Type 17 cytokines and reduced IL-1? and IL-18 at the site of infection
    Authors: GR Kathamuthu, K Moideen, D Baskaran, VV Banurekha, D Nair, G Sekar, R Sridhar, B Vidyajayan, G Gajendrara, DK Parandhama, A Srinivasan, S Babu
    Clin. Vaccine Immunol, 2017-05-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Bortezomib treatment sensitizes oncolytic HSV-1 treated tumors to NK cell immunotherapy
    Clin Cancer Res, 2016-07-07;0(0):.
    Species: Mouse
    Sample Types: Serum
  25. The Inflammasome and the Epidermal Growth Factor Receptor (EGFR) Are Involved in the Staphylococcus aureus-Mediated Induction of IL-1alpha and IL-1beta in Human Keratinocytes.
    Authors: Simanski M, Rademacher F, Schroder L, Glaser R, Harder J
    PLoS ONE, 2016-01-25;11(1):e0147118.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
    Authors: Haddad L, Sirois M
    PLoS ONE, 2014-02-12;9(2):e88980.
    Species: Human
    Sample Types: Cell Lysates
  27. Differential type 1 interferon-regulated gene expression in the brain during AIDS: interactions with viral diversity and neurovirulence.
    Authors: Polyak M, Vivithanaporn P, Maingat F, Walsh J, Branton W, Cohen E, Meeker R, Power C
    FASEB J, 2013-04-22;27(7):2829-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33.
    J. Exp. Med., 2012-07-16;209(8):1505-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
    Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
    Cytokine, 2005-07-21;31(2):153-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
    Authors: Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF
    Circ. Res., 2003-08-28;93(6):515-22.
    Species: Human
    Sample Types: Nuclear Extract

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Reviews for Human IL-1 alpha/IL-1F1 DuoSet ELISA

Average Rating: 4.6 (Based on 5 Reviews)

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Human IL-1 alpha/IL-1F1 DuoSet ELISA
By Anonymous on 02/11/2020
Sample Tested: K562 human chronic myelogenous leukemia cell line
Human IL-1 alpha/IL-1F1 DuoSet ELISA
By Carmen Martin-Ruiz on 08/15/2019
Sample Tested: Plasma

Very good kit, used on Human LiHep Plasma, 384-well plate format, sample used at 1/10 dilution and 4PL fitting.

Human IL-1 alpha/IL-1F1 DuoSet ELISA DY200
Human IL-1 alpha/IL-1F1 DuoSet ELISA
By BOCHATON Thomas on 08/29/2017
Sample Tested: Serum

Excellent ELISA.
We used on human serum with no dilution.

Human IL-1 alpha/IL-1F1 DuoSet ELISA DY200
Human IL-1 alpha/IL-1F1 DuoSet ELISA
By Anonymous on 05/01/2017
Sample Tested: Cell culture supernatant

Excellent

Human IL-1 alpha/IL-1F1 DuoSet ELISA DY200
Human IL-1 alpha/IL-1F1 DuoSet ELISA
By Anonymous on 11/28/2016
Sample Tested: Human Plasma