Human/Mouse CD44 PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB6127P-025
Detection of CD44 in Human PBMCs by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse CD44 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CD44 in flow cytometry.
Source
Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Myeloid leukemia M1 cells induced with Dexamethasone
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD44 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE‑conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Entrez Gene IDs
960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names
CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I

Product Datasheets

You must select a language.

x

Citation for Human/Mouse CD44 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer
    Authors: JD Twomey, B Zhang
    Biomedicines, 2023-03-17;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse CD44 PE-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse CD44 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse CD44 PE-conjugated Antibody
By Tahmineh Safaie on 11/08/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse