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Human/Mouse Olig2 Biotinylated Antibody

Catalog #: BAF2418
2 Citations Datasheet / COA / SDS
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BAF2418
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Olig2 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects Olig2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Olig1 and rhOlig3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Olig2
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse spinal cord (E11)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig2

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Long Name
Oligodendrocyte Lineage Transcription Factor 2
Entrez Gene IDs
10215 (Human)
Alternate Names
BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; Olig2; OLIGO2; oligodendrocyte lineage transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17; RACK17helix-loop-helix protein, class B, 1

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Citations for Human/Mouse Olig2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation
    Authors: A Xu, M Liu, MF Huang, Y Zhang, R Hu, JA Gingold, Y Liu, D Zhu, CS Chien, WC Wang, Z Liao, F Yuan, CW Hsu, J Tu, Y Yu, T Rosen, F Xiong, P Jia, YP Yang, DA Bazer, YW Chen, W Li, CD Huff, JJ Zhu, F Aguilo, SH Chiou, NC Boles, CC Lai, MC Hung, Z Zhao, EL Van Nostra, R Zhao, DF Lee
    Nature Communications, 2023-03-27;14(1):1694.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Spatiotemporally Dependent Vascularization Is Differently Utilized among Neural Progenitor Subtypes during Neocortical Development
    Authors: M Komabayash, E Yamanishi, C Watanabe, M Okamura, H Tabata, R Iwai, I Ajioka, J Matsushita, H Kidoya, N Takakura, T Okamoto, K Kinoshita, M Ichihashi, KI Nagata, M Ema, KI Mizutani
    Cell Rep, 2019-10-29;29(5):1113-1129.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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