Human/Mouse Pygopus-1 Antibody

Name: Human/Mouse Pygopus-1 Antibody
Brand: R&D Systems
SKU: MAB3617
Rating: 5 (1 reviews)

Catalog #: MAB3617 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3617
MAB3617-SP

Detection of Human and Mouse Pygopus‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Pygopus-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 466023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Pygopus‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Pygopus-1 Mono-clonal Antibody (Catalog # MAB3617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Pygopus-1

Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta -catenin in the nucleus during Wnt-signaling. In particular, following beta -catenin activation and translocation to the nucleus, beta -catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta -catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.

Entrez Gene IDs

26108 (Human); 72135 (Mouse)

Alternate Names

DKFZp547G0910; Pygo1; pygopus 1; pygopus homolog 1 (Drosophila); pygopus homolog 1; Pygopus1; Pygopus-1; pygopus-like protein 1