Home / 11 beta-HSD1 / Human/Mouse/Rat 11 beta-HSD1 Antibody

Human/Mouse/Rat 11 beta-HSD1 Antibody

Catalog #: AF3397
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3397
AF3397-SP
Detection of Human/Mouse/Rat 11 beta -HSD1 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat 11 beta-HSD1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat 11 beta -HSD1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 11 beta -HSD1
Asn24-Lys292
Accession # P28845
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat 11 beta -HSD1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat 11 beta -HSD1 by Western Blot. Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat 11 beta -HSD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3397) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for 11 beta -HSD1 at approximately 36 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 11 beta-HSD1

Hydoxysteroid (11-beta) Dehydrogenase, type I 11-beta-hydroxysteroid dehydrogenase 1(11 beta -HSD1, HSD11B1 or HSD1) is a microsomal glycoprotein enzyme, with an apparent mass of 36 kDa, that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. 11 beta -HSD1 can also catalyze the reverse reaction, the conversion of cortisone to cortisol. 11 beta -HSD1 has been detected in a wide range of rat and human tissues, including lung and testis, but highest amounts are expressed in the liver. Human 11 beta -HSD1 shares 79 and 77% sequence identity with mouse and rat 11 beta -HSD1, respectively. There are at least 2 isoforms of 11 beta -HSD. The other isoform, type II, is expressed predominantly in the kidney and placenta and catalyzes only the 11-beta-dehydrogenation reaction. High levels of cortisol can lead to visceral obesity and particular defects in the expression or function of 11 beta -HSD1 have also been associated with insulin resistance and cognitive function.

Long Name
11 beta Hydroxysteroid Dehydrogenase 1
Entrez Gene IDs
3290 (Human); 628438 (Mouse); 24470 (Rat)
Alternate Names
11 betaHSD1; 11 beta-HSD1; 11-beta-hydroxysteroid dehydrogenase 1; EC 1.1.1; EC 1.1.1.146; HDL; HSD11B1; HSD11HSD11B; HSD11LMGC13539; hydroxysteroid (11-beta) dehydrogenase 1; member 1; SDR26C1

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Citations for Human/Mouse/Rat 11 beta-HSD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Corticosterone and Glucocorticoid Receptor in the Cortex of Rats during Aging-The Effects of Long-Term Food Restriction
    Authors: V Tesic, J Ciric, I Jovanovic, N Zogovic, D Milanovic, S Kanazir, M Perovic
    Nutrients, 2021-12-17;13(12):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. NAFLD Aggravates Septic Shock Due to Inadequate Adrenal Response and 11beta-HSDs Dysregulation in Rats
    Authors: HC Huang, MH Tsai, FY Lee, TY Lin, CC Chang, CL Chuang, SJ Hsu, MC Hou, YH Huang
    Pharmaceutics, 2020-04-28;12(5):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Saturated fatty acids in human visceral adipose tissue are associated with increased 11- beta-hydroxysteroid-dehydrogenase type 1 expression.
    Authors: Petrus P, Rosqvist F, Edholm D, Mejhert N, Arner P, Dahlman I, Ryden M, Sundbom M, Riserus U
    Lipids Health Dis, 2015-05-02;14(0):42.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Identification of cytochrome P450 2C2 protein complexes in mouse liver.
    Authors: Li B, Yau P, Kemper B
    Proteomics, 2011-08-01;11(16):3359-68.
    Species: Rabbit
    Sample Types: Recombinant Protein
    Applications: Immunoprecipitation

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