Human/Mouse/Rat FoxP3 PE-conjugated Antibody
Human/Mouse/Rat FoxP3 PE-conjugated Antibody Summary
Gln105-Lys200
Accession # Q9BZS1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Rat Anti-Mouse CD4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB554G). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Rat Anti-Mouse CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB2438A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of FoxP3 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8970P) and Anti-Rat CD4 APC-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP3
Human FoxP3 is a 431 amino acid (aa), 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three alternative splicing events have been described in human. The first shows a deletion of aa 72-106, a second exhibits a deletion of aa 246-272, and a third records a 60 aa insertion after Lys382. These events are likely not to be mutually exclusive. The first isoform (plus the full-length form) occurs in CD4+CD25+ T cells, while the less frequent second isoform appears in CD8+ Tregs. The mouse FoxP3 gene does not appear to be spliced. In addition, FoxP3 is found only in Tregs in mouse, while human FoxP3 appears in both Tregs and CD4+ and CD8+ conventional T cells.
Both mouse and human FoxP3 undergo proteolytic processing, after Arg51 and Arg417 in human, and at equivalent sites in mouse. Cleavage at the N-terminus generate a 41 kDa isoform, whereas C-terminal cleavage generates an isoform indistinguishable from the full-length isoform (47 kDa) in SDS-PAGE.
FoxP3 can act as a transcriptional repressor (for IL-2) via an interaction with HDAC7. By contrast, it can also act as a transcriptional activator (for GITRL and
CTLA-4) through an interaction with RUNX7. Notably, for both human and mouse, full-length 47 kDa FoxP3 will induce high levels of CTLA-4 on expressing cells. However, when the C-terminally truncated isoform (at Arg417) is considered, IL-10 promoting activity is enhanced in mouse, while suppressive activity is lost in human. Over aa 105-200, human FoxP3 shares 83% and 84% aa sequence identity with rat and mouse FoxP3, respectively.
Product Datasheets
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