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Human/Mouse/Rat Olig2 Antibody

Catalog #: AF2418
95 Citations 4 Reviews Datasheet / COA / SDS
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AF2418
AF2418-SP
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Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells.
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Human/Mouse/Rat Olig2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Olig2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Olig2
Met1-Lys323
Accession # Q13516
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Olig2
Immunohistochemistry
5-15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Olig2 and Oligodendrocyte Marker O4 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Chromatin Immunoprecipitation (ChIP) Detection of Olig2-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation. A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.

Immunohistochemistry Olig2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Olig2 in Mouse Embryo. Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

Olig2 in Human Brain. Olig2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Olig2 in Rat Midbrain. Olig2 was detected in immersion fixed paraffin-embedded sections of rat midbrain using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig2

Olig1 and Olig2 are basic-helix-loop-helix (bHLH) transcription factors expressed in the motor neuron progenitor (pMN) domain of the spinal cord that generates motor neurons and oligodendrocytes. Olig1 is involved in the development and maturation of oligodendrocytes. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord.

Long Name
Oligodendrocyte Lineage Transcription Factor 2
Entrez Gene IDs
10215 (Human)
Alternate Names
BHLHB1; BHLHE19; Class B basic helix-loop-helix protein 1; Class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; Olig2; OLIGO2; oligodendrocyte lineage transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C binding protein 2; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17; RACK17helix-loop-helix protein, class B, 1

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Citations for Human/Mouse/Rat Olig2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
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  1. CD4+CD25+ regulatory T cells ex vivo generated from autologous naïve CD4+ T cells suppress EAE progression
    Authors: Yang, TT;Liu, PJ;Sun, QY;Wang, ZY;Yuan, GB;Fan, ZX;Ma, L;Lu, JF;Yuan, BY;Zou, WL;Zhao, LM;Li, Q;Liu, GZ;
    Scientific reports
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development
    Authors: Abdel Fattah, AR;Kolaitis, N;Van Daele, K;Daza, B;Rustandi, AG;Ranga, A;
    Nature communications
    Species: Mouse
    Sample Types: Organoid
    Applications: Immunohistochemistry
  3. Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-kappaB pathway in LPC-induced demyelination
    Authors: Y Wang, D Sadike, B Huang, P Li, Q Wu, N Jiang, Y Fang, G Song, L Xu, W Wang, M Xie
    Journal of Neuroinflammation, 2023-02-18;20(1):41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice
    Authors: H Park, B Cho, H Kim, T Saito, TC Saido, KJ Won, J Kim
    Nature Communications, 2023-02-13;14(1):802.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Early Permissiveness of Central Nervous System Cells to Measles Virus Infection Is Determined by Hyperfusogenicity and Interferon Pressure
    Authors: M Ferren, A Lalande, M Iampietro, L Canus, D Decimo, D Gerlier, M Porotto, C Mathieu
    Viruses, 2023-01-13;15(1):.
    Species: Hamster - Mesocricetus auratus (Golden Hamster)
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease
    Authors: S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu
    Acta neuropathologica communications, 2022-12-21;10(1):188.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. An atlas of late prenatal human neurodevelopment resolved by single-nucleus transcriptomics
    Authors: SI Ramos, ZM Mussa, EN Falk, B Pai, B Giotti, K Allette, P Cai, F Dekio, R Sebra, KG Beaumont, AM Tsankov, NM Tsankova
    Nature Communications, 2022-12-12;13(1):7671.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC/IF
  8. CHRDL1 Regulates Stemness in Glioma Stem-like Cells
    Authors: I Berglar, S Hehlgans, A Wehle, C Roth, C Herold-Men, F Rödel, D Kögel, B Linder
    Cells, 2022-12-03;11(23):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Phloretin enhances remyelination by stimulating oligodendrocyte precursor cell differentiation
    Authors: T Dierckx, S Vanherle, M Haidar, E Grajchen, F Mingneau, P Gervois, E Wolfs, D Bylemans, A Voet, T Nguyen, I Hamad, M Kleinewiet, JFJ Bogie, JJA Hendriks
    Proceedings of the National Academy of Sciences of the United States of America, 2022-11-07;119(46):e2120393119.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. A single-cell transcriptome atlas of glial diversity in the human hippocampus across the postnatal lifespan
    Authors: Y Su, Y Zhou, ML Bennett, S Li, M Carceles-C, L Lu, S Huh, D Jimenez-Cy, BC Kennedy, SK Kessler, AN Viaene, I Helbig, X Gu, JE Kleinman, TM Hyde, DR Weinberger, DW Nauen, H Song, GL Ming
    Cell Stem Cell, 2022-11-03;29(11):1594-1610.e8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice
    Authors: T Zhou, Y Li, X Li, F Zeng, Y Rao, Y He, Y Wang, M Liu, D Li, Z Xu, X Zhou, S Du, F Niu, J Peng, X Mei, SJ Ji, Y Shu, W Lu, F Guo, T Wu, TF Yuan, Y Mao, B Peng
    Nature Communications, 2022-10-24;13(1):6233.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. High-resolution transcriptomics informs glial pathology in human temporal lobe epilepsy
    Authors: B Pai, J Tome-Garci, WS Cheng, G Nudelman, KG Beaumont, S Ghatan, F Panov, E Caballero, K Sarpong, L Marcuse, J Yoo, Y Jiang, A Schaefer, S Akbarian, R Sebra, D Pinto, E Zaslavsky, NM Tsankova
    Acta neuropathologica communications, 2022-10-23;10(1):149.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system
    Authors: N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi
    Nature Communications, 2022-10-13;13(1):6040.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. A TCF7L2-responsive suppression of both homeostatic and compensatory remyelination in Huntington disease mice
    Authors: A Benraiss, JN Mariani, A Tate, PM Madsen, KM Clark, KA Welle, R Solly, L Capellano, K Bentley, D Chandler-M, SA Goldman
    Cell Reports, 2022-08-30;40(9):111291.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Expression of antioxidant enzymes in lesions of multiple sclerosis and its models
    Authors: D Moezzi, Y Dong, RW Jain, BM Lozinski, S Ghorbani, C D'Mello, V Wee Yong
    Scientific Reports, 2022-07-26;12(1):12761.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Molecular landscapes of human hippocampal immature neurons across lifespan
    Authors: Y Zhou, Y Su, S Li, BC Kennedy, DY Zhang, AM Bond, Y Sun, F Jacob, L Lu, P Hu, AN Viaene, I Helbig, SK Kessler, T Lucas, RD Salinas, X Gu, HI Chen, H Wu, JE Kleinman, TM Hyde, DW Nauen, DR Weinberger, GL Ming, H Song
    Nature, 2022-07-06;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats
    Authors: Q Liu, S Zhou, X Wang, C Gu, Q Guo, X Li, C Zhang, N Zhang, L Zhang, F Huang
    Journal of Neuroinflammation, 2022-06-20;19(1):160.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  18. High-Throughput Analysis of Astrocyte Cultures Shows Prevention of Reactive Astrogliosis by the Multi-Nutrient Combination Fortasyn Connect
    Authors: A Badia-Sote, J de Vries, W Dykstra, LM Broersen, JM Verkuyl, AB Smit, MHG Verheijen
    Cells, 2022-04-22;11(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties
    Authors: C Azevedo, G Teku, Y Pomeshchik, JF Reyes, M Chumarina, K Russ, E Savchenko, A Hammarberg, NJ Lamas, A Collin, GK Gouras, O Klementiev, M Hallbeck, R Taipa, M Vihinen, L Roybon
    Proceedings of the National Academy of Sciences of the United States of America, 2022-03-16;119(12):e2111405119.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  20. TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss
    Authors: T Feng, L Luan, II Katz, M Ullah, VM Van Deerli, JQ Trojanowsk, EB Lee, F Hu
    Acta neuropathologica communications, 2022-03-14;10(1):33.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development
    Authors: W Wang, H Cho, JW Lee, SK Lee
    Nature Communications, 2022-02-17;13(1):958.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia
    Authors: T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström
    EBioMedicine, 2022-02-16;77(0):103882.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. BMP10 expression in the adult rat central nervous system
    Authors: C Ogawa, S Mikawa, S Li, Y Hayashi, K Masumoto, K Sato
    Journal of chemical neuroanatomy, 2022-02-16;121(0):102084.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  24. A gut-derived metabolite alters brain activity and anxiety behaviour in mice
    Authors: BD Needham, M Funabashi, MD Adame, Z Wang, JC Boktor, J Haney, WL Wu, C Rabut, MS Ladinsky, SJ Hwang, Y Guo, Q Zhu, JA Griffiths, R Knight, PJ Bjorkman, MG Shapiro, DH Geschwind, DP Holschneid, MA Fischbach, SK Mazmanian
    Nature, 2022-02-14;602(7898):647-653.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells
    Authors: Q Wu, Y Shichino, T Abe, T Suetsugu, A Omori, H Kiyonari, S Iwasaki, F Matsuzaki
    Nature Communications, 2022-01-25;13(1):470.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Extracellular Vesicles Derived from Young Neural Cultures Attenuate Astrocytic Reactivity In Vitro
    Authors: D Almansa, H Peinado, R García-Rod, Á Casadomé-P, CG Dotti, FX Guix
    International Journal of Molecular Sciences, 2022-01-25;23(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  27. A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)
    Authors: WU Park, GB Yeon, MS Yu, HG Goo, SH Hwang, D Na, DS Kim
    Biology, 2021-12-01;10(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Cuprizone feed formulation influences the extent of demyelinating disease pathology
    Authors: LM Toomey, M Papini, B Lins, AJ Wright, A Warnock, T McGonigle, SC Hellewell, CA Bartlett, C Anyaegbu, M Fitzgerald
    Scientific Reports, 2021-11-19;11(1):22594.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. IL-33 induces orofacial neuropathic pain through Fyn-dependent phosphorylation of GluN2B in the trigeminal spinal nucleus caudalis
    Authors: Y Kimura, Y Hayashi, S Hitomi, D Ikutame, K Urata, I Shibuta, A Sakai, J Ni, K Iwata, M Tonogi, M Shinoda
    Brain, Behavior, and Immunity, 2021-10-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Microglial ASD-related genes are involved in oligodendrocyte differentiation
    Authors: Y Takanezawa, S Tanabe, D Kato, R Ozeki, M Komoda, T Suzuki, H Baba, R Muramatsu
    Scientific Reports, 2021-09-08;11(1):17825.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. A Novel Mutation in Cse1l Disrupts Brain and Eye Development with Specific Effects on Pax6 Expression
    Authors: LE Blizzard, C Menke, SD Patel, RR Waclaw, SA Lachke, RW Stottmann
    Journal of developmental biology, 2021-07-07;9(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Nanoparticulate matter exposure results in white matter damage and an inflammatory microglial response in an experimental murine model
    Authors: M Connor, K Lamorie-Fo, Q Liu, K Shkirkova, H Baertsch, C Sioutas, TE Morgan, CE Finch, WJ Mack
    PLoS ONE, 2021-07-02;16(7):e0253766.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Generation of excitatory and inhibitory neurons from common progenitors via Notch signaling in the cerebellum
    Authors: T Zhang, T Liu, N Mora, J Guegan, M Bertrand, X Contreras, AH Hansen, C Streicher, M Anderle, N Danda, L Tiberi, S Hippenmeye, BA Hassan
    Cell Reports, 2021-06-08;35(10):109208.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
    Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
    JCI Insight, 2021-05-10;6(9):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Co-Immunoprecipitation, IHC, Western Blot
  35. Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice
    Authors: W Mu, S Li, J Xu, X Guo, H Wu, Z Chen, L Qiao, G Helfer, F Lu, C Liu, QF Wu
    Nature Communications, 2021-04-16;12(1):2288.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells
    Authors: JE Lee, S Ahn, H Jeong, S An, CH Myung, JA Lee, SC Hong, YJ Kim, JY Kim, JH Ryu, M Noh, KT Nam, JS Hwang
    Scientific Reports, 2021-04-08;11(1):7778.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  37. Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4
    Authors: BY Fan, YL Pang, WX Li, CX Zhao, Y Zhang, X Wang, GZ Ning, XH Kong, C Liu, X Yao, SQ Feng
    Neural regeneration research, 2021-03-01;16(3):561-566.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Oxidized phosphatidylcholines found in multiple sclerosis lesions mediate neurodegeneration and are neutralized by microglia
    Authors: Y Dong, C D'Mello, W Pinsky, BM Lozinski, DK Kaushik, S Ghorbani, D Moezzi, D Brown, FC Melo, S Zandee, T Vo, A Prat, SN Whitehead, VW Yong
    Nature Neuroscience, 2021-02-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. BMP9 expression in the adult rat brain
    Authors: C Ogawa, S Mikawa, Y Hayashi, K Masumoto, F Katou, K Sato
    Journal of chemical neuroanatomy, 2021-02-11;113(0):101933.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  40. AIM2 inflammasome mediates hallmark neuropathological alterations and cognitive impairment in a mouse model of vascular dementia
    Authors: L Poh, DY Fann, P Wong, HM Lim, SL Foo, SW Kang, V Rajeev, S Selvaraji, VR Iyer, N Parathy, MB Khan, DC Hess, DG Jo, GR Drummond, CG Sobey, MKP Lai, CL Chen, LHK Lim, TV Arumugam
    Molecular Psychiatry, 2020-12-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  41. SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells
    Authors: W Tao, A Zhang, K Zhai, Z Huang, H Huang, W Zhou, Q Huang, X Fang, BC Prager, X Wang, Q Wu, AE Sloan, MS Ahluwalia, JD Lathia, JS Yu, JN Rich, S Bao
    EMBO Mol Med, 2020-10-30;0(0):e12291.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Quantitative in vivo bioluminescence imaging of orthotopic patient-derived glioblastoma xenografts
    Authors: AL Koessinger, D Koessinger, K Stevenson, C Cloix, L Mitchell, C Nixon, N Gomez-Roma, AJ Chalmers, JC Norman, SWG Tait
    Scientific Reports, 2020-09-21;10(1):15361.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  43. PRC2 Acts as a Critical Timer That Drives Oligodendrocyte Fate over Astrocyte Identity by Repressing the Notch Pathway
    Authors: W Wang, H Cho, D Kim, Y Park, JH Moon, SJ Lim, SM Yoon, M McCane, SA Aicher, S Kim, B Emery, JW Lee, S Lee, Y Park, SK Lee
    Cell Rep, 2020-09-15;32(11):108147.
    Species: Chicken, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. Single-Cell Profiling Shows Murine Forebrain Neural Stem Cells Reacquire a Developmental State when Activated for Adult Neurogenesis
    Authors: MJ Borrett, BT Innes, D Jeong, N Tahmasian, MA Storer, GD Bader, DR Kaplan, FD Miller
    Cell Rep, 2020-08-11;32(6):108022.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: FISH, IHC
  45. BCAS1-positive immature oligodendrocytes are affected by the &alpha-synuclein-induced pathology of multiple system atrophy
    Authors: S Kaji, T Maki, J Ueda, T Ishimoto, Y Inoue, K Yasuda, M Sawamura, R Hikawa, T Ayaki, H Yamakado, R Takahashi
    Acta Neuropathol Commun, 2020-07-29;8(1):120.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  46. EEF1A1 deacetylation enables transcriptional activation of remyelination
    Authors: M Duman, A Vaquié, G Nocera, M Heller, M Stumpe, D Siva Sanka, J Dengjel, D Meijer, T Yamaguchi, P Matthias, T Zeis, N Schaeren-W, A Hayoz, S Ruff, C Jacob
    Nat Commun, 2020-07-09;11(1):3420.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  47. Ginseng gintonin alleviates neurological symptoms in the G93A-SOD1 transgenic mouse model of amyotrophic lateral sclerosis through lysophosphatidic acid 1 receptor
    Authors: SM Nam, JH Choi, SH Choi, HJ Cho, YJ Cho, H Rhim, HC Kim, IH Cho, DG Kim, SY Nah
    Journal of Ginseng Research, 2020-05-01;45(3):390-400.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. A Matter of Choice: Inhibition of c-Rel Shifts Neuronal to Oligodendroglial Fate in Human Stem Cells
    Authors: LM Ruiz-Perer, JFW Greiner, C Kaltschmid, B Kaltschmid
    Cells, 2020-04-22;9(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Spatiotemporal Decline of BMP Signaling Activity in Neural Progenitors Mediates Fate Transition and Safeguards Neurogenesis
    Authors: TC Ma, KI Vong, KM Kwan
    Cell Rep, 2020-03-17;30(11):3616-3624.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  50. Pathological modeling of TBEV infection reveals differential innate immune responses in human neurons and astrocytes that correlate with their susceptibility to infection
    Authors: M Fares, M Cochet-Ber, G Gonzalez, CN Montero-Me, O Blanchet, A Benchoua, C Boissart, S Lecollinet, J Richardson, N Haddad, M Coulpier
    J Neuroinflammation, 2020-03-03;17(1):76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Ginseng Gintonin Attenuates Lead-Induced Rat Cerebellar Impairments during Gestation and Lactation
    Authors: SM Nam, SH Choi, HJ Cho, JS Seo, M Choi, SS Nahm, BJ Chang, SY Nah
    Biomolecules, 2020-03-02;10(3):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  52. Follistatin expression in the central nervous system of the adult rat
    Authors: C Ogawa, S Mikawa, K Yamashita, Y Hayashi, K Masumoto, F Katou, K Sato
    J. Chem. Neuroanat., 2020-01-31;105(0):101753.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  53. Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and TREM2-independent cellular responses in Alzheimer's disease
    Authors: Y Zhou, WM Song, PS Andhey, A Swain, T Levy, KR Miller, PL Poliani, M Cominelli, S Grover, S Gilfillan, M Cella, TK Ulland, K Zaitsev, A Miyashita, T Ikeuchi, M Sainouchi, A Kakita, DA Bennett, JA Schneider, MR Nichols, SA Beausoleil, JD Ulrich, DM Holtzman, MN Artyomov, M Colonna
    Nat. Med., 2020-01-13;26(1):131-142.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  54. Cortical astrocytes develop in a plastic manner at both clonal and cellular levels
    Authors: S Clavreul, L Abdeladim, E Hernández-, D Niculescu, J Durand, SH Ieng, R Barry, G Bonvento, E Beaurepair, J Livet, K Loulier
    Nat Commun, 2019-10-25;10(1):4884.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  55. Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection
    Authors: LM Toomey, CA Bartlett, N Gavriel, T McGonigle, M Majimbi, G Gopalasing, J Rodger, M Fitzgerald
    Sci Rep, 2019-10-25;9(1):15297.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  56. Chronic disturbance in the thalamus following cranial irradiation to the developing mouse brain
    Authors: M Boström, Y Eriksson, J Danial, T Björk-Erik, M Kalm
    Sci Rep, 2019-07-03;9(1):9588.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  57. The effects of a combination of ion channel inhibitors on pathology in a model of demyelinating disease
    Authors: G Gopalasing, CA Bartlett, T McGonigle, M Majimbi, A Warnock, A Ford, A Gough, LM Toomey, M Fitzgerald
    Mult Scler Relat Disord, 2019-06-08;34(0):1-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  58. BMP6 expression in the adult rat central nervous system
    Authors: Y Hayashi, S Mikawa, C Ogawa, K Masumoto, F Katou, K Sato
    J. Chem. Neuroanat., 2019-03-27;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  59. Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury
    Authors: V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix
    Nat Commun, 2019-01-31;10(1):518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  60. Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation
    Authors: M Osipovitch, A Asenjo Mar, JN Mariani, A Cornwell, S Dhaliwal, L Zou, D Chandler-M, S Wang, X Li, SJ Benraiss, R Agate, A Lampp, A Benraiss, MS Windrem, SA Goldman
    Cell Stem Cell, 2018-12-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  61. Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis
    Authors: AM Falcão, D van Brugge, S Marques, M Meijer, S Jäkel, E Agirre, Samudyata, EM Floriddia, DP Vanichkina, C Ffrench-Co, A Williams, AO Guerreiro-, G Castelo-Br
    Nat. Med., 2018-11-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  62. NF-?B p65 directs sex-specific neuroprotection in human neurons
    Authors: LM Ruiz-Perer, L Schneider, BA Windmöller, J Müller, JFW Greiner, C Kaltschmid, B Kaltschmid
    Sci Rep, 2018-10-30;8(1):16012.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  63. Myostatin expression in the adult rat central nervous system
    Authors: Y Hayashi, S Mikawa, C Ogawa, K Masumoto, F Katou, K Sato
    J. Chem. Neuroanat., 2018-10-24;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  64. Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate
    Authors: Q Chen, J Wu, Q Ye, F Ma, Q Zhu, Y Wu, C Shan, X Xie, D Li, X Zhan, C Li, XF Li, X Qin, T Zhao, H Wu, PY Shi, J Man, CF Qin
    MBio, 2018-09-18;9(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  65. Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice
    Authors: F Cathomas, D Azzinnari, G Bergamini, H Sigrist, M Buerge, V Hoop, B Wicki, L Goetze, S Soares, D Kukelova, E Seifritz, S Goebbels, KA Nave, M Said Ghand, C Seoighe, T Hildebrand, G Leparc, H Klein, E Stupka, B Hengerer, CR Pryce
    Genes Brain Behav., 2018-04-17;0(0):e12475.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  66. A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors
    Authors: T Srivastava, P Diba, JM Dean, F Banine, D Shaver, M Hagen, X Gong, W Su, B Emery, DL Marks, EN Harris, B Baggenstos, PH Weigel, LS Sherman, SA Back
    J. Clin. Invest., 2018-04-16;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  67. Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner
    Authors: M Datta, O Staszewski, E Raschi, M Frosch, N Hagemeyer, TL Tay, T Blank, M Kreutzfeld, D Merkler, S Ziegler-Wa, P Matthias, M Meyer-Lueh, M Prinz
    Immunity, 2018-03-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  68. Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy
    Authors: W Zhou, C Chen, Y Shi, Q Wu, RC Gimple, X Fang, Z Huang, K Zhai, SQ Ke, YF Ping, H Feng, JN Rich, JS Yu, S Bao, XW Bian
    Cell Stem Cell, 2017-11-02;21(5):591-603.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  69. The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1
    Authors: FI Young, M Keruzore, X Nan, N Gennet, EJ Bellefroid, M Li
    Proc. Natl. Acad. Sci. U.S.A., 2017-06-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  70. Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors
    Authors: NJ Yates, MK Giacci, RL O'Hare Doi, W Chiha, BE Ashworth, J Kenna, CA Bartlett, M Fitzgerald
    Neural Regen Res, 2017-02-01;12(2):307-316.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  71. Brain tissue banking for stem cells for our future
    Sci Rep, 2016-12-19;6(0):39394.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  72. The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation
    Authors: Majistor Raj Luxman Maglo Renkilaraj
    Neurobiol. Dis, 2016-12-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  73. Patched1 and Patched2 inhibit Smoothened non-cell autonomously
    Elife, 2016-08-23;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  74. A cord blood monocyte-derived cell therapy product accelerates brain remyelination
    JCI Insight, 2016-08-18;1(13):e86667.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  75. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation
    Authors: Qi Xie
    J Clin Invest, 2016-06-20;126(7):2757-2772.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  76. Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression
    J Leukoc Biol, 2016-05-06;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  77. Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors
    Authors: SH Choi, SH Kim, KW Shim, JW Han, J Choi, DS Kim, CJ Lyu, JW Kim, CO Suh, J Cho
    PLoS ONE, 2016-04-13;11(4):e0153443.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  78. Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro.
    Authors: Mendes F, Coelho Aguiar J, Kahn S, Reis A, Dubois L, Romao L, Ferreira L, Chneiweiss H, Moura Neto V, Abreu J
    PLoS ONE, 2015-08-04;10(8):e0133689.
    Species: Human
    Sample Types: Cell Lysates
  79. A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
    Authors: Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent M, Veiga-da-Cunha M, Van Schaftingen E
    PLoS ONE, 2015-03-12;10(3):e0119540.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  80. Mitochondrial control by DRP1 in brain tumor initiating cells.
    Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
    Nat Neurosci, 2015-03-02;18(4):501-10.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  81. Notch signaling regulates motor neuron differentiation of human embryonic stem cells.
    Authors: Ben-Shushan E, Feldman E, Reubinoff B
    Stem Cells, 2015-02-01;33(2):403-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells.
    Authors: Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L
    Stem Cells, 2014-09-01;32(9):2539-49.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  83. Phage display discovery of novel molecular targets in glioblastoma-initiating cells.
    Authors: Liu, J K, Lubelski, D, Schonberg, D L, Wu, Q, Hale, J S, Flavahan, W A, Mulkearns-Hubert, E E, Man, J, Hjelmeland, A B, Yu, J, Lathia, J D, Rich, J N
    Cell Death Differ, 2014-05-16;21(8):1325-39.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  84. Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
    Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J
    Genes Dev, 2014-05-01;28(10):1085-100.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  85. Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum.
    Authors: Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue Y, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson M, Wright C, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M
    Nat Commun, 2014-01-01;5(0):3337.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  86. BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells.
    Authors: Srikanth, Maya, Kim, Juno, Das, Sunit, Kessler, John A
    Mol Cancer Res, 2013-11-22;12(2):283-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  87. Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy.
    Authors: Piltti K, Salazar D, Uchida N, Cummings B, Anderson A
    Stem Cells Transl Med, 2013-02-14;2(3):204-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  88. Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination.
    Authors: Kuboyama, Kazuya, Fujikawa, Akihiro, Masumura, Makoto, Suzuki, Ryoko, Matsumoto, Masahito, Noda, Masaharu
    PLoS ONE, 2012-11-07;7(11):e48797.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  89. The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Authors: Zelinka C, Scott M, Volkov L, Fischer A
    PLoS ONE, 2012-09-04;7(9):e44477.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  90. N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells.
    Authors: Martinez-Cerdeno V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS
    PLoS ONE, 2012-06-26;7(6):e39456.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  91. Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.
    Authors: Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ
    PLoS ONE, 2010-08-18;5(8):e12272.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  92. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007-11-01;505(1):73-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  93. Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice.
    Authors: Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M
    Stem Cells, 2007-07-12;25(10):2619-27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  94. Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain.
    Authors: Bauer S, Patterson PH
    J. Neurosci., 2006-11-15;26(46):12089-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Reviews for Human/Mouse/Rat Olig2 Antibody

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Human/Mouse/Rat Olig2 Antibody
By Anonymous on 07/09/2019
Application: ICC/IF Sample Tested: Spinal cord Species: Mouse

This antibody worked nicely 1:500 or 1:1000 of mouse spinal cord perfused with 4% PFA.

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Olig2 Antibody AF2418
Human/Mouse/Rat Olig2 Antibody
By Anonymous on 06/20/2018
Application: IHC Sample Tested: Adult brain Species: Mouse

This antibody worked beautifully at 1:2000 in 30um free-floating sections of adult (P25) mouse brain perfused with 4% PFA and cryoprotected. Region imaged: corpus callosum above hippocampus.

Protocol:
1. Antigen Retrieval: 100mM Sodium Citrate pH6.0, 0.05% Tween, in microwave
2. Rinse sections PBS once
3. Block in 5% NDS in PBSTx 1% RT for 1 hour
4. Primary Antibody (1:2000) in 1% NDS in PBSTx 0.3% (Incubate RT overnight on shaker)
5. Wash 3x 10 min PBS
6. Secondary Antibody (1:600) in 1% NDS in PBSTx 0.3% (Incubate RT 2h on shaker)
7. Wash 3x 10 min PBS
8. Mount on slides in water and let dry
9. Coverslip using Prolong Gold

Immunohistochemistry Human/Mouse/Rat Olig2 Antibody AF2418
Human Olig2 Antibody
By Anonymous on 01/04/2018
Application: ICC/IF Sample Tested: Glioma Species: Human

Glioma xenografted in NOD SCID mice -> olig2 in red

Immunocytochemistry/Immunofluorescence Human Olig2 Antibody AF2418
Human Olig2 Antibody
By Anonymous on 12/07/2015
Application: Affinity Purification Sample Tested: Brain sections Species: Rat Sprague Dawley

Dilution 1:500