Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody

Catalog #: AF6340 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6340
AF6340-SP

Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects recombinant human POR/Cytochrome P450 Reductase in direct ELISAs and human, mouse, and rat POR/Cytochrome P450 Reductase in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived human POR/Cytochrome P450 Reductase
Arg45-Ser677
Accession # P16435

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat POR/Cytochrome P450 Reductase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POR/Cytochrome P450 Reductase

NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b₅, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.

References

Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
Flueck C.E. et al. (2004) Nat. Genet. 36:228.

Long Name

NADPH Cytochrome P450 Reductase

Entrez Gene IDs

5447 (Human)

Alternate Names

CPR; CYPOR; CYPORFLJ26468; Cytochrome P450 Reductase; DKFZp686G04235; EC 1.6.2.4; NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; P450R; POR