Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody
Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody Summary
Met1-Ile497
Accession # Q16881
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Thioredoxin Reductase 1/TRXR1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thioredoxin Reductase 1/TRXR1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Thioredoxin Reductase 1/TRXR1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Monoclonal Antibody (Catalog # MAB7428, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thioredoxin Reductase 1/TRXR1
Thioredoxin reductase 1 (TRXR1) is an approximately 70 kDa member of the class-I pyridine nucleotide-disulfide oxidoreductase family. Human TRXR1 is 649 amino acids (aa) in length. Residues 151-152 constitute a propeptide that is deleted from the mature protein. Splicing variants produce five additional isoforms for human TRXR1. Isoform 2 has a 32 aa substitution for aa 107-138 and a deletion of aa 1-106. Isoform 3 has a deletion of aa 1-51 and a 49 aa substitution for aa 52-100. Isoform 4 is missing aa 1-98 and has a 3 aa substitution for aa 99-101. Isoform 5 has a deletion of aa 1-150. Residues 56-156 make up a glutaredoxin domain, and residues 520-632 constitute a pyridine nucleotide-disulphide oxidoreductase dimerization domain. In addition, there are three phosphotyrosines at positions 161, 163, and 281, and a selenocysteine at position 648. Human TRXR1 shares 74% and 70% aa sequence identity with mouse and rat TRXR1, respectively. Isoform 1 is involved in glutaredoxin activity as well as thioredoxin reductase activity, and it induces actin and tubulin polymerization, which leads to formation of cell membrane protrusions. Isoform 4 has been shown to enhance the transcriptional activity of the beta receptor only. Finally, isoform 5 mediates cell death induced by a combination of interferon-beta and retinoic acid. Isoform 1 is expressed mostly in the Leydig cells, but also in the ovary, spleen, heart, liver, kidney, and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in the kidney, uterus, testis, ovary, prostate, placenta, and fetal liver.
Product Datasheets
Citations for Human/Mouse/Rat Thioredoxin Reductase 1/TRXR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Co-Targeting of BTK and TrxR as a Therapeutic Approach to the Treatment of Lymphoma
Authors: Sicong Wang, Erin Clapper, Kathryn F. Tonissen, Giovanna Di Trapani
Antioxidants (Basel)
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Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen
Authors: Sara Evke, Qishan Lin, Juan Andres Melendez, Thomas John Begley
Genes (Basel)
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The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene
Authors: Andrea Leonardi, Nataliia Kovalchuk, Lei Yin, Lauren Endres, Sara Evke, Steven Nevins et al.
Epigenetics
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Alterations of redox and iron metabolism accompany the development of HIV latency
Authors: Iart Luca Shytaj, Bojana Lucic, Mattia Forcato, Carlotta Penzo, James Billingsley, Vibor Laketa et al.
The EMBO Journal
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Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor
Authors: Y Fan, M Makar, MX Wang, HW Ai
Nat. Chem. Biol., 2017-06-26;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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