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Human/Mouse/Rat Vimentin Antibody

Catalog #: MAB2105
21 Citations 5 Reviews Datasheet / COA / SDS
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MAB2105
MAB2105-SP
Detection of Human Vimentin by Western Blot.
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Citations (21)
FAQs
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Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Vimentin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. Detects human, mouse and rat Vimentin in Western blots.
Source
Monoclonal Rat IgG2A Clone # 280618
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Vimentin
Ser2-Glu466
Accession # P08670
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1-2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
Vimentin is specifically detected in K562 human chronic myelogenous leukemia parental cell line but is not detectable in Vimentin knockout K562 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Vimentin antibody by Western Blot. View Larger

Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse and Rat Vimentin antibody by Western Blot. View Larger

Detection of Mouse and Rat Vimentin by Western Blot. Western blot shows lysates of MEF mouse embryonic feeder cells, NIH-3T3 mouse embryonic fibroblast cell line, Rat-2 rat embryonic fibroblast cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Vimentin antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Vimentin in NTera‑2 Human Cell Line. Vimentin was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Vimentin antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

Vimentin in A549 Human Cell Line. Vimentin was detected in immersion fixed A549 human lung carcinoma cell line using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Vimentin antibody in Mouse Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Vimentin in Mouse Cortical Stem Cells. Vimentin was detected in immersion fixed mouse cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Vimentin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Vimentin in Rat Cortical Stem Cells. Vimentin was detected in immersion fixed rat cortical stem cells using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Vimentin antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

Vimentin in Human Tonsil. Vimentin was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Vimentin antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Vimentin in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human Vimentin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Vimentin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Vimentin at approximately 58 kDa (as indicated) using 10 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human Vimentin Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Vimentin Specificity by Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Vimentin knockout K562 cell line (KO). PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (Catalog # MAB2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in knockout K562 cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

References
  1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.
  2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.
  3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.
  4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.
  5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.
  6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.
  7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.
  8. Styers, M.L. et al. (2005) Traffic 6:359.
  9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.
  10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.
  11. Ivaska, J. et al. (2005) EMBO J. 24:3834.
  12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.
  13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.
  14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.
  15. Garg, A. et al. (2006) J. Immunol. 177:6192.
Entrez Gene IDs
7431 (Human); 22352 (Mouse); 81818 (Rat)
Alternate Names
epididymis secretory sperm binding protein; FLJ36605; VIM; Vimentin

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Citations for Human/Mouse/Rat Vimentin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia
    Authors: Park, GT;Lim, JK;Choi, EB;Lim, MJ;Yun, BY;Kim, DK;Yoon, JW;Hong, YG;Chang, JH;Bae, SH;Ahn, JY;Kim, JH;
    Biomaterials research
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus
    Authors: MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher
    Elife, 2022-11-07;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
    Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
    Cell Reports, 2022-09-27;40(13):111423.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. NR2F1 is a barrier to dissemination of early stage breast cancer cells
    Authors: C Rodriguez-, N Kale, MJ Carlini, N Shrivastav, AA Rodrigues, B Khalil, JJ Bravo-Cord, M Alexander, J Ji, Y Hong, F Behbod, MS Sosa
    Cancer Research, 2022-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IF
  5. Suspension culture promotes serosal mesothelial development in human intestinal organoids
    Authors: MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence
    Cell Reports, 2022-02-15;38(7):110379.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  6. The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease.
    Authors: de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O'Shea E
    Elife, 2021-09-30;10(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance
    Authors: M Cui, A Atmanli, MG Morales, W Tan, K Chen, X Xiao, L Xu, N Liu, R Bassel-Dub, EN Olson
    Nature Communications, 2021-09-06;12(1):5270.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Echinochrome A Treatment Alleviates Fibrosis and Inflammation in Bleomycin-Induced Scleroderma
    Authors: GT Park, JW Yoon, SB Yoo, YC Song, P Song, HK Kim, J Han, SJ Bae, KT Ha, NP Mishchenko, SA Fedoreyev, VA Stonik, MB Kim, JH Kim
    Marine Drugs, 2021-04-23;19(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis
    Authors: D Yamakawa, D Katoh, K Kasahara, T Shiromizu, M Matsuyama, C Matsuda, Y Maeno, M Watanabe, Y Nishimura, M Inagaki
    Cell Reports, 2021-03-09;34(10):108817.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  10. Heterogeneous Manifestations of Epithelial-Mesenchymal Plasticity of Circulating Tumor Cells in Breast Cancer Patients
    Authors: LA Tashireva, OE Savelieva, ES Grigoryeva, YV Nikitin, EV Denisov, SV Vtorushin, MV Zavyalova, NV Cherdyntse, VM Perelmuter
    International Journal of Molecular Sciences, 2021-03-02;22(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity
    Authors: SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang
    Nature Communications, 2021-01-11;12(1):262.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  12. Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution
    Authors: Z Wang, M Cui, AM Shah, W Tan, N Liu, R Bassel-Dub, EN Olson
    Cell Reports, 2020-12-08;33(10):108472.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Quantitative proteomic comparison of myofibroblasts derived from bone marrow and cornea
    Authors: P Saikia, JS Crabb, LL Dibbin, MJ Juszczak, B Willard, GF Jang, TM Shiju, JW Crabb, SE Wilson
    Sci Rep, 2020-10-07;10(1):16717.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: ICC
  14. Sensitive and easy screening for circulating tumor cells by flow cytometry
    Authors: A Lopresti, F Malergue, F Bertucci, ML Liberatosc, S Garnier, Q DaCosta, P Finetti, M Gilabert, JL Raoul, D Birnbaum, C Acquaviva, E Mamessier
    JCI Insight, 2019-06-13;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Platelet glycoprotein VI and C-type lectin-like receptor 2 deficiency accelerates wound healing by impairing vascular integrity in mice
    Authors: S Wichaiyo, S Lax, SJ Montague, Z Li, B Grygielska, JA Pike, EJ Haining, A Brill, SP Watson, J Rayes
    Haematologica, 2019-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. RNA sequencing reveals upregulation of a transcriptomic program associated with stemness in metastatic prostate cancer cells selected for taxane resistance
    Authors: CK Cajigas-Du, SR Martinez, L Woods-Burn, AM Durán, S Roy, A Basu, JA Ramirez, GL Ortiz-Hern, L Ríos-Colón, E Chirshev, ES Sanchez-He, U Soto, C Greco, C Boucheix, X Chen, J Unternaehr, C Wang, CA Casiano
    Oncotarget, 2018-07-13;9(54):30363-30384.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Acute Drug Effects on the Human Placental Tissue: The Development of a Placental Murine Xenograft Model
    Authors: M Verheecke, E Hermans, S Tuyaerts, E Souche, R Van Bree, G Verbist, T Everaert, J Van Houdt, K Van Calste, F Amant
    Reprod Sci, 2018-02-13;0(0):1933719118756.
    Species: Xenograft
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
    Authors: Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G
    Nat Cell Biol, 2015-08-24;17(9):1193-204.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015-07-24;195(5):2429-41.
  20. GLI1, CTNNB1 and NOTCH1 protein expression in a thymic epithelial malignancy tissue microarray.
    Authors: Riess J, West R, Dean M, Klimowicz A, Neal J, Hoang C, Wakelee H
    2015-02-01;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.
    Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
    Invest. Ophthalmol. Vis. Sci., 2008-08-15;49(12):5325-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human/Mouse/Rat Vimentin Antibody

Average Rating: 4.4 (Based on 5 Reviews)

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Human/Mouse/Rat Vimentin Antibody
By Anonymous on 06/19/2022
Application: ICC/IF Sample Tested: Epithelial cells Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Vimentin Antibody MAB2105
Human/Mouse/Rat Vimentin Antibody
By cristian De Gregorio on 06/15/2021
Application: ICC/IF Sample Tested: Human fibroblast Species: Human

Vimentin expression in human fibroblasts in culture. Vimentin (green) was used as a fibroblast marker in miofibroblast differentiation assays (a-SMA in red, as a miofibroblast marker).

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Vimentin Antibody MAB2105
Human/Mouse/Rat Vimentin Antibody
By Anonymous on 02/26/2021
Application: ICC/IF Sample Tested: Mouse fibroblast cell line Species: Mouse
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat Vimentin Antibody MAB2105
Human/Mouse/Rat Vimentin Antibody
By Tyler Crowe on 06/23/2020
Application: IHC Sample Tested: Skin tissue Species: Rhesus Macaque
Immunohistochemistry Human/Mouse/Rat Vimentin Antibody MAB2105
Human/Mouse/Rat Vimentin Antibody
By Tara Peters on 11/14/2019
Application: WB Sample Tested: Cancer cell lysates Species: Human

Testing several cell lines for mesenchymal characteristics, including vimentin expression, by western blot.

Western Blot Human/Mouse/Rat Vimentin Antibody MAB2105