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Human Nestin Antibody

Catalog #: MAB1259
100 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1259
MAB1259-SP
Nestin in Human Neural Progenitor Cells.
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Product Details
Citations (100)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nestin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nestin.
Source
Monoclonal Mouse IgG1 Clone # 196908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
A172 human glioblastoma cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nestin antibody in Human Neural Progenitor Cells by Immunocytochemistry (ICC). View Larger

Nestin in Human Neural Progenitor Cells. Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry View Larger

Detection of Nestin in A172 cells by Flow Cytometry. A172 cells were stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

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Citations for Human Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

100 Citations: Showing 1 - 10
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  1. A simplified protocol for the generation of cortical brain organoids
    Authors: KN Eigenhuis, HB Somsen, M van der Kr, H Smeenk, AL Korporaal, SA Kushner, FMS de Vrij, DLC van den Be
    Frontiers in Cellular Neuroscience, 2023-04-04;17(0):1114420.
    Species: Human
    Sample Types: Organoid
    Applications: ICC
  2. The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2
    Authors: EJ Kim, JY Kim, SO Kim, N Hong, SH Choi, MG Park, J Jang, SW Ham, S Seo, SY Lee, K Lee, HJ Jeong, SJ Kim, S Jeong, K Min, SC Kim, X Jin, SH Kim, SH Kim, H Kim
    Cell Reports, 2022-11-22;41(8):111626.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Vestibular Nuclei: A New Neural Stem Cell Niche?
    Authors: G Rastoldo, I Watabe, A Lapotre, A Tonetto, A López-Juár, B Tighilet
    Cells, 2022-11-14;11(22):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells
    Authors: L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak
    Nature Communications, 2022-09-19;13(1):5494.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases
    Authors: J Brás, D Henriques, R Moreira, MM Santana, R Silva-Pedr, D Adão, S Braz, AR Álvaro, LP de Almeida, LS Mendonça
    Scientific Reports, 2022-07-22;12(1):12513.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  6. Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
    Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
    International Journal of Molecular Sciences, 2022-06-30;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
    Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
    Oncogene, 2022-06-14;63(0):102845.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiation
    Authors: YW Cho, S Jee, IR Suhito, JH Lee, CG Park, KM Choi, TH Kim
    Science Advances, 2022-04-20;8(16):eabj7736.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ICC
  9. Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene
    Authors: M Tisch, M Carmen De, M Suarez-Cub, C Fauth, M Defrancesc, J Zschocke, K Günther, F Edenhofer
    Stem Cell Research, 2022-04-09;61(0):102784.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Steady-State Levels of Miro1 Linked to Phosphorylation at Serine 156 and Mitochondrial Respiration in Dopaminergic Neurons
    Authors: L Schwarz, JC Fitzgerald
    Cells, 2022-04-08;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis
    Authors: K Kaushal, EJ Kim, A Tyagi, JK Karapurkar, S Haq, HS Jung, KS Kim, S Ramakrishn
    Cell Death and Differentiation, 2022-03-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma
    Authors: B Polat, G Wohlleben, R Kosmala, D Lisowski, F Mantel, V Lewitzki, M Löhr, R Blum, P Herud, M Flentje, CM Monoranu
    Cancer Cell International, 2022-02-19;22(1):87.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IF, IHC
  13. Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells
    Authors: B Bohl, A Jabali, J Ladewig, P Koch
    Science Advances, 2022-02-11;8(6):eabl5792.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function
    Authors: PA Barbuti, J Ohnmacht, BFR Santos, PM Antony, F Massart, G Cruciani, CM Dording, L Pavelka, N Casadei, YJ Kwon, R Krüger
    Scientific Reports, 2021-11-09;11(1):21946.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development
    Authors: Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen
    Stem Cell Research, 2021-10-22;57(0):102583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  16. Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations
    Authors: J Gil-Ranedo, C Gallego-Ga, JM Almendral
    Cell Reports, 2021-09-07;36(10):109673.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Rapid target validation in a Cas9-inducible hiPSC derived kidney model
    Authors: Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks
    Scientific Reports, 2021-08-16;11(1):16532.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  18. Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells
    Authors: S Rajasingh, V Sigamani, V Selvam, N Gurusamy, S Kirankumar, J Vasanthan, J Rajasingh
    Journal of Cellular and Molecular Medicine, 2021-08-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability
    Authors: F Xiong, R Wang, JH Lee, S Li, SF Chen, Z Liao, LA Hasani, PT Nguyen, X Zhu, J Krakowiak, DF Lee, L Han, KL Tsai, Y Liu, W Li
    Cell Research, 2021-06-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Restoring miR-132 expression rescues adult hippocampal neurogenesis and memory deficits in Alzheimer's disease
    Authors: H Walgrave, S Balusu, S Snoeck, E Vanden Eyn, K Craessaert, N Thrupp, L Wolfs, K Horré, Y Fourne, A Ronisz, E Silajdži?, A Penning, G Tosoni, Z Callaerts-, R D'Hooge, DR Thal, H Zetterberg, S Thuret, M Fiers, CS Frigerio, B De Stroope, E Salta
    Cell Stem Cell, 2021-05-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity
    Authors: MJ Ruiz-Magañ, R Martinez-A, T Llorca, AC Abadia-Mol, C Ruiz-Ruiz, EG Olivares
    Journal of reproductive immunology, 2021-05-01;145(0):103326.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  22. Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants
    Authors: RB Bressan, B Southgate, KM Ferguson, C Blin, V Grant, N Alfazema, JC Wills, MA Marques-To, GM Morrison, J Ashmore, F Robertson, CAC Williams, L Bradley, A von Kriegs, RA Anderson, SR Tomlinson, SM Pollard
    Cell Stem Cell, 2021-02-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Activity-dependent regulome of human GABAergic neurons reveals new patterns of gene regulation and neurological disease heritability
    Authors: GL Boulting, E Durresi, B Ataman, MA Sherman, K Mei, DA Harmin, AC Carter, DR Hochbaum, AJ Granger, JM Engreitz, S Hrvatin, MR Blanchard, MG Yang, EC Griffith, ME Greenberg
    Nature Neuroscience, 2021-02-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. Ex-vivo recellularisation and stem cell differentiation of a decellularised rat dental pulp matrix
    Authors: M Matoug-Elw, H Nazzal, EM Raif, SP Wilshaw, F Esteves, M Duggal
    Scientific Reports, 2020-12-09;10(1):21553.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
    Authors: A Colmenero-, MA Gómez-Muño, I Rodríguez-, A Amador-Álv, KO Henrich, D Pascual-Va, K Okonechnik, E Rivas, F Westermann, R Pardal, FM Vega
    Cancers (Basel), 2020-11-20;12(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. The HIF1&alpha/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation
    Authors: LR Gauthier, M Saati, H Bensalah-P, K Ben M'Bare, O Gitton-Que, R Bertrand, D Busso, MA Mouthon, A Collura, MP Junier, H Chneiweiss, JR Pineda, FD Boussin
    Sci Rep, 2020-10-30;10(1):18742.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Generation of a human iPSC line CIBi008-A from amniotic fluid-derived cells of a fetus with &beta-thalassemia carrying variants of -28A�>�G and IVS-II-654C�>�T in HBB
    Authors: S Wu, H Wei, M Chu, Z Weng
    Stem Cell Res, 2020-10-29;49(0):102074.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P
    Authors: P Barbuti, P Antony, B Santos, F Massart, G Cruciani, C Dording, J Arias, J Schwamborn, R Krüger
    Cells, 2020-09-10;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. EGFR/FOXO3a/BIM signaling pathway determines chemosensitivity of BMP4-differentiated glioma stem cells to temozolomide
    Authors: IA Ciechomska, B Gielniewsk, B Wojtas, B Kaminska, J Mieczkowsk
    Exp. Mol. Med., 2020-08-12;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Protein
    Applications: Western Blot
  30. Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats
    Authors: CC Liang, SS Shaw, YS Ko, YH Huang, TH Lee
    Sci Rep, 2020-06-22;10(1):10030.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  31. Development of immortalized human hepatocyte-like hybrid cells by fusion of multi-lineage progenitor cells with primary hepatocytes
    Authors: DP Collins, JH Hapke, RN Aravalli, CJ Steer
    PLoS ONE, 2020-06-04;15(6):e0234002.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Human, Mouse
    Sample Types:
    Applications: Cell Culture, IF
  33. Multimodal Therapeutic Effects of Neural Precursor Cells Derived from Human-Induced Pluripotent Stem Cells through Episomal Plasmid-Based Reprogramming in a Rodent Model of Ischemic Stroke
    Authors: SH Oh, YW Jeong, W Choi, JE Noh, S Lee, HS Kim, J Song
    Stem Cells Int, 2020-03-23;2020(0):4061516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. FKBP9 promotes the malignant behavior of glioblastoma cells and confers resistance to endoplasmic reticulum stress inducers
    Authors: H Xu, P Liu, Y Yan, K Fang, D Liang, X Hou, X Zhang, S Wu, J Ma, R Wang, T Li, H Piao, S Meng
    J. Exp. Clin. Cancer Res., 2020-02-28;39(1):44.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  35. Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis
    Authors: M Huang, J Tailor, Q Zhen, AH Gillmor, ML Miller, H Weishaupt, J Chen, T Zheng, EK Nash, LK McHenry, Z An, F Ye, Y Takashima, J Clarke, H Ayetey, FMG Cavalli, B Luu, BS Moriarity, S Ilkhanizad, L Chavez, C Yu, KM Kurian, T Magnaldo, N Sevenet, P Koch, SM Pollard, P Dirks, MP Snyder, DA Largaespad, YJ Cho, JJ Phillips, FJ Swartling, AS Morrissy, M Kool, SM Pfister, MD Taylor, A Smith, WA Weiss
    Cell Stem Cell, 2019-06-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  36. Direct Reprogramming of Human Neurons Identifies MARCKSL1 as a Pathogenic Mediator of Valproic Acid-Induced Teratogenicity
    Authors: S Chanda, CE Ang, QY Lee, M Ghebrial, D Haag, Y Shibuya, M Wernig, TC Südhof
    Cell Stem Cell, 2019-05-30;25(1):103-119.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats
    Authors: C Zalfa, L Rota Nodar, E Vacchi, M Gelati, D Profico, M Boido, E Binda, L De Filippi, M Copetti, V Garlatti, P Daniele, J Rosati, A De Luca, F Pinos, L Cajola, A Visioli, L Mazzini, A Vercelli, M Svelto, AL Vescovi, D Ferrari
    Cell Death Dis, 2019-04-25;10(5):345.
    Species: Xenograft
    Sample Types: Whole Tissue
    Applications: IHC
  38. Characterization of the first induced pluripotent stem cell line generated from a patient with autosomal dominant catecholaminergic polymorphic ventricular tachycardia due to a heterozygous mutation in cardiac calsequestrin-2
    Authors: S Ross, M Holliday, S Lim, C Semsarian
    Stem Cell Res, 2019-04-25;37(0):101450.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain
    Authors: J Luzuriaga, O Pastor-Alo, JM Encinas, F Unda, G Ibarretxe, JR Pineda
    Front Physiol, 2019-03-28;10(0):347.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
    Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
    Sci Rep, 2019-03-20;9(1):4902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes
    Authors: I Bertolini, A Terrasi, C Martelli, G Gaudioso, A Di Cristof, AM Storaci, M Formica, P Braidotti, K Todoerti, S Ferrero, M Caroli, L Ottobrini, T Vaccari, V Vaira
    EBioMedicine, 2019-02-06;0(0):.
    Species: Human
    Sample Types: Tissue Array
    Applications: IHC
  42. Aberrant neuronal differentiation is common in glioma but is associated neither with epileptic seizures nor with better survival
    Authors: CP Beier, T Rasmussen, RH Dahlrot, HB Tenstad, JS Aarø, MF Sørensen, SB Heimisdótt, MD Sørensen, P Svenningse, MJ Riemenschn, D Beier, BW Kristensen
    Sci Rep, 2018-10-08;8(1):14965.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation
    Authors: M Holliday, SB Ross, S Lim, C Semsarian
    Stem Cell Res, 2018-10-06;33(0):56-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies
    Authors: J Rosati, D Ferrari, F Altieri, S Tardivo, C Ricciolini, C Fusilli, C Zalfa, DC Profico, F Pinos, L Bernardini, B Torres, I Manni, G Piaggio, E Binda, M Copetti, G Lamorte, T Mazza, M Carella, M Gelati, EM Valente, A Simeone, AL Vescovi
    Cell Death Dis, 2018-09-17;9(10):937.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  45. GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System
    Authors: R Tirughana, MZ Metz, Z Li, C Hall, D Hsu, J Beltzer, AJ Annala, D Oganesyan, M Gutova, KS Aboody
    Mol Ther Methods Clin Dev, 2018-07-07;10(0):48-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  46. Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime
    Authors: S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto
    J Transl Med, 2018-06-01;16(1):148.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  47. Genome Editing in Neuroepithelial Stem Cells to Generate Human Neurons with High Adenosine-Releasing Capacity
    Authors: D Poppe, J Doerr, M Schneider, R Wilkens, JA Steinbeck, J Ladewig, A Tam, DE Paschon, PD Gregory, A Reik, CE Müller, P Koch, O Brüstle
    Stem Cells Transl Med, 2018-03-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers
    Authors: M Shafa, F Yang, T Fellner, MS Rao, BA Baghbadera
    Front Med (Lausanne), 2018-03-15;5(0):69.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease
    Authors: L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker
    PLoS ONE, 2018-03-07;13(3):e0192497.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  50. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria
    Authors: F Hsu, S Spannl, C Ferguson, AA Hyman, RG Parton, M Zerial
    Elife, 2018-02-22;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  51. Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness
    Authors: H Yao, H Zhao, J Wang, GG Haddad
    Neuroscience, 2018-02-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea
    Authors: D Ghoubay-Be, C de Sousa, R Martos, G Latour, MC Schanne-Kl, E Dupin, V Borderie
    PLoS ONE, 2017-11-17;12(11):e0188398.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
    Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
    Oncogene, 2017-09-18;37(3):286-301.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
    Authors: AM Rosager, MD Sørensen, RH Dahlrot, S Hansen, DL Schonberg, JN Rich, JD Lathia, BW Kristensen
    PLoS ONE, 2017-08-24;12(8):e0182954.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  55. Induction of senescence in primary glioblastoma cells by serum and TGF?
    Authors: R Kumar, A Gont, TJ Perkins, JEL Hanson, IAJ Lorimer
    Sci Rep, 2017-05-19;7(1):2156.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  57. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017-04-25;12(4):e0174074.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017-04-04;19(1):20-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  59. Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
    Authors: SS Jensen, SA Petterson, B Halle, C Aaberg-Jes, BW Kristensen
    BMC Cancer, 2017-03-07;17(1):178.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  60. Generation of induced pluripotent stem cells (iPSCs) from a hypertrophic cardiomyopathy patient with the pathogenic variant p.Val698Ala in beta-myosin heavy chain (MYH7) gene
    Authors: SB Ross, ST Fraser, N Nowak, C Semsarian
    Stem Cell Res, 2017-03-07;20(0):88-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  61. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells
    Authors: HO King, T Brend, HL Payne, A Wright, TA Ward, K Patel, T Egnuni, LF Stead, A Patel, H Wurdak, SC Short
    Stem Cell Reports, 2017-01-10;8(1):125-139.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  62. Cytosine-5 RNA Methylation Regulates Neural Stem Cell Differentiation and�Motility
    Stem Cell Reports, 2016-12-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  63. Expression and Prognostic Value of Oct-4 in Astrocytic Brain Tumors
    PLoS ONE, 2016-12-28;11(12):e0169129.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  64. BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells
    Sci Rep, 2016-12-09;6(0):38723.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  65. Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization
    Authors: Florian Wegner
    J. Neurosci., 2016-11-23;36(47):12027-12043.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  66. Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype
    PLoS ONE, 2016-05-12;11(5):e0155106.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  67. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015-04-01;33(4):1142-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  68. The evidence of glioblastoma heterogeneity.
    Authors: Soeda, Akio, Hara, Akira, Kunisada, Takahiro, Yoshimura, Shin-ich, Iwama, Toru, Park, Deric M
    Sci Rep, 2015-01-27;5(0):7979.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  69. A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT).
    Authors: Jeitany M, Pineda J, Liu Q, Porreca R, Hoffschir F, Desmaze C, Silvestre D, Mailliet P, Junier M, Londono-Vallejo A, Segal-Bendirdjian E, Chneiweiss H, Boussin F
    Int J Cancer, 2014-09-11;136(7):1546-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  70. Molecular evidence for OCT4-induced plasticity in adult human fibroblasts required for direct cell fate conversion to lineage specific progenitors.
    Authors: Mitchell R, Szabo E, Shapovalova Z, Aslostovar L, Makondo K, Bhatia M
    Stem Cells, 2014-08-01;32(8):2178-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  71. Phenotypic plasticity in normal breast derived epithelial cells.
    Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
    BMC Cell Biol, 2014-06-10;15(0):20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  72. Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
    Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
    Int J Oncol, 2014-01-24;44(4):1118-30.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  73. Novel anti-glioblastoma agents and therapeutic combinations identified from a collection of FDA approved drugs.
    Authors: Jiang, Pengfei, Mukthavaram, Rajesh, Chao, Ying, Bharati, Ila Sri, Fogal, Valentin, Pastorino, Sandra, Cong, Xiuli, Nomura, Natsuko, Gallagher, Matt, Abbasi, Taher, Vali, Shireen, Pingle, Sandeep, Makale, Milan, Kesari, Santosh
    J Transl Med, 2014-01-17;12(0):13.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  74. Epigenetic silencing of miRNA-9 is associated with HES1 oncogenic activity and poor prognosis of medulloblastoma.
    Authors: Fiaschetti G, Abela L, Nonoguchi N, Dubuc A, Remke M, Boro A, Grunder E, Siler U, Ohgaki H, Taylor M, Baumgartner M, Shalaby T, Grotzer M
    Br J Cancer, 2013-12-17;110(3):636-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  75. Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
    Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
    PLoS ONE, 2013-03-22;8(3):e59252.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  76. Embryonic stem cell-based modeling of tau pathology in human neurons.
    Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P
    Am J Pathol, 2013-03-13;182(5):1769-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  77. Activation of the type I interferon pathway is enhanced in response to human neuronal differentiation.
    Authors: Farmer, Jocelyn, Altschaefl, Kate M, O'Shea, K Sue, Miller, David J
    PLoS ONE, 2013-03-07;8(3):e58813.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  78. Nestin is highly expressed in advanced-stage melanomas and neurotized nevi.
    Authors: Akiyama, Michiko, Matsuda, Yoko, Ishiwata, Toshiyuk, Naito, Zenya, Kawana, Seiji
    Oncol Rep, 2013-02-15;29(4):1595-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  79. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012-05-02;7(5):e35639.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  80. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2011-07-26;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  81. Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
    Authors: Engelen E, Akinci U, Bryne JC
    Nat. Genet., 2011-05-01;43(6):607-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
    Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
    J. Invest. Dermatol., 2010-09-30;131(2):487-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  83. Glial cell type-specific subcellular localization of 14-3-3 zeta: an implication for JCV tropism.
    Authors: Lamba S, Ravichandran V, Major EO
    Glia, 2009-07-01;57(9):971-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Induction of oligodendrogenesis in glioblastoma-initiating cells by IFN-mediated activation of STAT3 signaling.
    Authors: Yuki K, Natsume A, Yokoyama H, Kondo Y, Ohno M, Kato T, Chansakul P, Ito M, Kim SU, Wakabayashi T
    Cancer Lett., 2009-05-19;284(1):71-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  85. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009-01-20;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  86. Development of a reconstructed cornea from collagen-chondroitin sulfate foams and human cell cultures.
    Authors: Vrana NE, Builles N, Justin V, Bednarz J, Pellegrini G, Ferrari B, Damour O, Hulmes DJ, Hasirci V
    Invest. Ophthalmol. Vis. Sci., 2008-08-15;49(12):5325-31.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  87. Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
    Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
    Stem Cells, 2008-04-17;26(7):1705-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  88. The chemokine CXCL16 induces migration and invasion of glial precursor cells via its receptor CXCR6.
    Authors: Hattermann K, Ludwig A, Gieselmann V, Held-Feindt J, Mentlein R
    Mol. Cell. Neurosci., 2008-04-10;39(1):133-41.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  89. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture.
    Authors: Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, Smith A
    Mol. Cell. Neurosci., 2008-03-18;38(2):245-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  90. The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress.
    Authors: Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J
    Neurosci. Lett., 2007-08-03;423(3):225-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  91. Long-term maintenance of brain tumor stem cell properties under at non-adherent and adherent culture conditions.
    Authors: Inagaki A, Soeda A, Oka N, Kitajima H, Nakagawa J, Motohashi T, Kunisada T, Iwama T
    Biochem. Biophys. Res. Commun., 2007-07-18;361(3):586-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  92. Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro.
    Authors: Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C
    Exp. Hematol., 2007-07-01;35(7):1119-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  93. Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue.
    Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD
    Stem Cells, 2007-02-01;25(5):1298-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  94. Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
    Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
    Br. J. Haematol., 2006-12-01;135(5):703-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  95. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006-10-02;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  96. Impaired proliferation and migration in human Miller-Dieker neural precursors.
    Authors: Sheen VL, Ferland RJ, Harney M, Hill RS, Neal J, Banham AH, Brown P, Chenn A, Corbo J, Hecht J, Folkerth R, Walsh CA
    Ann. Neurol., 2006-07-01;60(1):137-44.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  97. Long-term proliferation of human embryonic stem cell-derived neuroepithelial cells using defined adherent culture conditions.
    Authors: Shin S, Mitalipova M, Noggle S, Tibbitts D, Venable A, Rao R, Stice SL
    Stem Cells, 2005-08-11;24(1):125-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  98. Three-dimensional porous alginate scaffolds provide a conducive environment for generation of well-vascularized embryoid bodies from human embryonic stem cells.
    Authors: Gerecht-Nir S, Cohen S, Ziskind A, Itskovitz-Eldor J
    Biotechnol. Bioeng., 2004-11-05;88(3):313-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  99. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  100. Differentiation of human embryonic stem cells into insulin-producing clusters.
    Authors: Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J
    Stem Cells, 2004-01-01;22(3):265-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human Nestin Antibody
By Anonymous on 06/17/2022
Application: ICC/IF Sample Tested: Neuroblastoma cells Species: Human
Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
Human Nestin Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cell Lysates Species: Human

Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 5% non-fat milk in TBST
Dilution: 1/1000

Western Blot Human Nestin Antibody MAB1259
Human Nestin Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: Human cell line Species: Human

Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.3% Triton X-100 in PBS
Dilution: 1/100

Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259
Human Nestin Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: HeLa cells Species: Human

Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: 1% BSA + 0.1% Triton X-100 in PBS
Dilution: 1/100

Immunocytochemistry/Immunofluorescence Human Nestin Antibody MAB1259