Human/Mouse Olig1 Antibody

Catalog # Availability Size / Price Qty
MAB2417
MAB2417-SP
Olig1 in Human Glioma.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Olig1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Olig1. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3.
Source
Monoclonal Mouse IgG2B Clone # 257219
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Olig1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Olig1 antibody in Human Glioma by Immunohistochemistry (IHC-P). View Larger

Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human Olig1 antibody by Western Blot. View Larger

Detection of Human Olig1 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Olig1

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Long Name
Oligodendrocyte Lineage Gene 1
Entrez Gene IDs
116448 (Human)
Alternate Names
BHLHB6; bHLHe21; Class B basic helix-loop-helix protein 6; Class E basic helix-loop-helix protein 21; helix-loop-helix protein, class B, 6; Olig1; oligo1; oligodendrocyte lineage transcription factor 1; oligodendrocyte transcription factor 1; oligodendrocyte-specific bHLH transcription factor 1

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Citations for Human/Mouse Olig1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Hedgehog Signalling Modulates Immune Response and Protects against Experimental Autoimmune Encephalomyelitis
    Authors: A Ballester, A Guijarro, B Bravo, J Hernández, R Murillas, MI Gallego, S Ballester
    International Journal of Molecular Sciences, 2022-03-15;23(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity
    Authors: S Yang, S Li, XJ Li
    Cell Rep, 2018-12-04;25(10):2653-2659.e3.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.
    Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C
    J Neurosci, 2015-01-07;35(1):4-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Reduced Morg1 expression in ischemic human brain.
    Authors: Haase D, Keiner S, Mawrin C, Wolf G
    Neurosci. Lett., 2009-03-18;455(1):46-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human/Mouse Olig1 Antibody
By safa azar on 06/08/2018
Application: ICC/IF Sample Tested: Brain (cerebral cortex),Cancer Tissue Species: Human