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Human PDGF R alpha APC-conjugated Antibody

Catalog #: FAB1264A
6 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1264A
Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF R alpha APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF R alpha. Recognizes the PDGF receptor alpha -subunit; it does not recognize the PDGF receptor beta -subunit. Binds to the PDGF receptor alpha -subunit of primate species (human, monkey, baboon) and dog. Does not recognize rat or mouse receptors and its ability to bind to receptors from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # PRa292
Purification
Protein A or G purified from ascites
Immunogen
Human osteosarcoma cell membrane extracts
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from ascites

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PDGF Ra antibody in U-118-MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PDGF R alpha in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human PDGF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB1264A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R alpha

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF‑BB and AB, but not PDGF-AA. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and heterodimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Only PDGF R alpha is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGF-R alpha has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF R alpha binds PDGF with high affinity and is a potent PDGF antagonist (1).

References
  1. Heldin, C.H. and L. Claesson-Welsh (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A. (ed) Oxford University Press, New York, NY p. 202.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

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Citations for Human PDGF R alpha APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
    Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
    Cell Reports, 2021-12-07;37(10):110095.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  2. Specific induction and long-term maintenance of high purity ventricular cardiomyocytes from human induced pluripotent stem cells
    Authors: H Fukushima, M Yoshioka, M Kawatou, V López-Dávi, M Takeda, Y Kanda, Y Sekino, Y Yoshida, JK Yamashita
    PLoS ONE, 2020-11-02;15(11):e0241287.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: MACS
  3. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis
    Authors: NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry
    Cell Rep, 2017-08-15;20(7):1597-1608.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification
    Nature, 2016-07-14;535(7611):294-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Generation of articular chondrocytes from human pluripotent stem cells.
    Authors: Craft A, Rockel J, Nartiss Y, Kandel R, Alman B, Keller G
    Nat Biotechnol, 2015-05-11;33(6):638-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human PDGF R alpha APC-conjugated Antibody
By Concepcion Estaras on 06/15/2016
Application: Flow Sample Tested: Human embryonic stem cells derived mesoderm Species: Human
Flow Cytometry Human PDGF R alpha APC-conjugated Antibody FAB1264A