Home / PDGF R alpha / Human Phospho-PDGF R alpha DuoSet IC ELISA

Human Phospho-PDGF R alpha DuoSet IC ELISA

Catalog #: DYC2114-2
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC2114E
DYC2114-5
DYC2114-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
R&D Systems DuoSet ELISAs
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Citations (7)
FAQs
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Phospho-PDGF R alpha DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human PDGF R alpha in cell lysates. An immobilized capture antibody specific for PDGF R alpha binds both phosphorylated and unphosphorylated PDGF R alpha. After washing away unbound material, an HRP-conjugated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF R alpha

PDGF R alpha (Platelet-Derived Growth Factor Receptor alpha) is a transmembrane receptor tyrosine kinase that can form homodimers or heterodimers with PDGF R beta when engaged by dimers of the PDGF ligands. PDGF R alpha binds to PDGF-AA, PDGF-AB, and PDGF-CC. It is strongly expressed in oligodendrocyte, lung, skin, and intestinal progenitor cells, at low levels in most mesenchymal cells, and can be induced by inflammation. PDGF R alpha function is important during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme. Dysregulated PDGF R alpha signaling can result in spina bifida or cleft palate-type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart, and skin (scleroderma).

Long Name:
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs:
5156 (Human); 18595 (Mouse)
Alternate Names:
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Citations for Human Phospho-PDGF R alpha DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Identification and characterization of PDGFRalpha+ mesenchymal progenitors in human skeletal muscle.
    Authors: Uezumi A, Fukada S, Yamamoto N, Ikemoto-Uezumi M, Nakatani M, Morita M, Yamaguchi A, Yamada H, Nishino I, Hamada Y, Tsuchida K
    Cell Death Dis, 2014-04-17;5(0):e1186.
    Species: Human
    Sample Types: Cell Lysates
  2. Antifibrotic and anti-inflammatory activity of the tyrosine kinase inhibitor nintedanib in experimental models of lung fibrosis.
    Authors: Wollin L, Maillet I, Quesniaux V, Holweg A, Ryffel B
    J Pharmacol Exp Ther, 2014-02-20;349(2):209-20.
    Species: Human
    Sample Types: Cell Lysates
  3. Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130Cas.
    Authors: Pellet-Many C, Frankel P, Evans IM, Herzog B, Junemann-Ramirez M, Zachary IC
    Biochem. J., 2011-05-01;435(3):609-18.
    Species: Human
    Sample Types: Cell Lysates
  4. Synergistic effects of imatinib and carboplatin on VEGF, PDGF and PDGF-Ralpha/beta expression in squamous cell carcinoma of the head and neck in vitro.
    Authors: Schultz JD, Rotunno S, Riedel F, Anders C, Erben P, Hofheinz RD, Faber A, Thorn C, Sommer JU, Hormann K, Sauter A
    Int. J. Oncol., 2011-01-18;38(4):1001-12.
    Species: Human
    Sample Types: Cell Lysates
  5. Lack of detection of agonist activity by antibodies to platelet-derived growth factor receptor alpha in a subset of normal and systemic sclerosis patient sera.
    Authors: Loizos N, Lariccia L, Weiner J, Griffith H, Boin F, Hummers L, Wigley F, Kussie P
    Arthritis Rheum., 2009-04-01;60(4):1145-51.
    Species: Porcine
    Sample Types: Cell Lysates
  6. Platelet-derived growth factor-alpha receptor activation is required for human cytomegalovirus infection.
    Authors: Soroceanu L, Akhavan A, Cobbs CS
    Nature, 2008-08-13;455(7211):391-5.
    Species: Human
    Sample Types: Cell Lysates
  7. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007-04-30;177(3):489-500.
    Species: Human
    Sample Types: Cell Lysates

FAQs

  1. Which phosphorylated sites are recognized by this assay?

    • This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

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