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Human/Rat GFAP Antibody

Catalog #: AF2594
7 Citations Datasheet / COA / SDS
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AF2594
AF2594-SP
Detection of Human and Rat GFAP by Western Blot.
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Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Rat GFAP Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat GFAP in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GFAP
Leu292-Met432
Accession # P14136
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
0.1-2 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat GFAP antibody by Western Blot. View Larger

Detection of Human and Rat GFAP by Western Blot. Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry beta ‑III Tubulin antibody in Rat Cortical Neurons and GFAP antibody in Rat Astrocytes by Immunocytochemistry (ICC). View Larger

beta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta -III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta -III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern-Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry GFAP antibody in Rat Astrocytes by Immunocytochemistry (ICC). View Larger

GFAP in Rat Astrocytes. GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry GFAP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

GFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human GFAP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human GFAP by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Rat GFAP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat GFAP by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFAP

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.

Long Name
Glial Fibrillary Acidic Protein
Entrez Gene IDs
2670 (Human); 14580 (Mouse); 24387 (Rat)
Alternate Names
ALXDRD; FLJ45472; GFAP astrocytes; GFAP; glial fibrillary acidic protein

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Citations for Human/Rat GFAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A single-cell transcriptome atlas of glial diversity in the human hippocampus across the postnatal lifespan
    Authors: Y Su, Y Zhou, ML Bennett, S Li, M Carceles-C, L Lu, S Huh, D Jimenez-Cy, BC Kennedy, SK Kessler, AN Viaene, I Helbig, X Gu, JE Kleinman, TM Hyde, DR Weinberger, DW Nauen, H Song, GL Ming
    Cell Stem Cell, 2022-11-03;29(11):1594-1610.e8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Anti-M�llerian Hormone Regulation of Synaptic Transmission in the Hippocampus Requires MAPK Signaling and Kv4.2 Potassium Channel Activity
    Authors: K Wang, F Xu, J Maylie, J Xu
    Frontiers in Neuroscience, 2021-12-16;15(0):772251.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery
    Authors: KM LaBarbera, C Limegrover, C Rehak, R Yurko, NJ Izzo, N Knezovich, E Watto, L Waybright, SM Catalano
    Journal of neuroscience methods, 2021-04-06;0(0):109180.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. APP upregulation contributes to retinal ganglion cell degeneration via JNK3
    Authors: C Liu, CW Zhang, Y Zhou, WQ Wong, LC Lee, WY Ong, SO Yoon, W Hong, XY Fu, TW Soong, EH Koo, LW Stanton, KL Lim, ZC Xiao, GS Dawe
    Cell Death Differ., 2017-12-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders
    Authors: KT Gurwitz, RJ Burman, BD Murugan, S Garnett, T Ganief, NC Soares, JV Raimondo, JM Blackburn
    Front Mol Neurosci, 2017-05-29;10(0):163.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012-05-23;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity?
    Authors: Leonoudakis D, Braithwaite SP, Beattie MS, Beattie EC
    Neuron Glia Biol., 2004-08-01;1(3):263-273.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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