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Human Smad5 Antibody

Catalog #: AF2250
2 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2250
AF2250-SP
Smad5 in Human Colon.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Smad5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Smad5
Val155-Arg258
Accession # Q99717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Smad5
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Smad5 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

Smad5 in Human Colon. Smad5 was detected in immersion fixed paraffin-embedded sections of human colon using 5 µg/mL Human Smad5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2250) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Smad5

Smad5 (Smad; an amalgam of the Sma gene in worm and the MAD gene in fruitfly) is a transcriptional modulator that is activated by BMP receptor kinases. Human Smad5 shares 99% amino acid sequence homology with the mouse or rat protein.

Long Name
Mothers Against DPP Homolog 5
Entrez Gene IDs
4090 (Human)
Alternate Names
Dwfc; hSmad5; JV5-1; JV5-1DKFZp781O1323; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5 (Drosophila); MADH5; MADH5mothers against decapentaplegic homolog 5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; SMA- and MAD-related protein 5; SMAD 5; SMAD family member 5DKFZp781C1895; SMAD, mothers against DPP homolog 5 (Drosophila); SMAD, mothers against DPP homolog 5; Smad5

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Citations for Human Smad5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Quantitative kinetics analysis of BMP2 uptake into cells and its modulation by BMP antagonists.
    Authors: Alborzinia H, Schmidt-Glenewinkel H, Ilkavets I, Breitkopf-Heinlein K, Cheng X, Hortschansky P, Dooley S, Wolfl S
    J Cell Sci, 2012-10-17;126(0):117-27.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
    Authors: Rai D, Kim SW, McKeller MR
    Proc. Natl. Acad. Sci. U.S.A., 2010-02-01;107(7):3111-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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Reviews for Human Smad5 Antibody

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Human Smad5 Antibody
By Anonymous on 11/20/2019
Application: WB Sample Tested: Mv1Lu mink lung epithelial cell line Species: Mink

probe with Smad 5 antibody at 0.5ug/mL. I observed a weak band at the expected MW but there were many extra bands in my samples.

Western Blot Human Smad5 Antibody AF2250