Human SOST/Sclerostin Antibody Summary
Gln24-Tyr213
Accession # Q9BQB4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SOST/Sclerostin by Western Blot. Western blot shows lysates of human cartilage tissue and human bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1406) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOST/Sclerostin
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Human and mouse SOST share 88% amino acid identity (1-3).
Product Datasheets
Citations for Human SOST/Sclerostin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Negative Association Between Sclerostin and INSL3 in Isolated Human Osteocytes and in Klinefelter Syndrome: New Hints for Testis-Bone Crosstalk
Authors: A Di Nisio, L De Toni, MS Rocca, M Ghezzi, R Selice, G Taglialavo, A Ferlin, C Foresta
J. Clin. Endocrinol. Metab., 2018-05-01;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC, Western Blot -
Sclerostin regulates release of bone mineral by osteocytes by induction of carbonic anhydrase 2.
Authors: Kogawa M, Wijenayaka A, Ormsby R, Thomas G, Anderson P, Bonewald L, Findlay D, Atkins G
J Bone Miner Res, 2013-12-01;28(12):2436-48.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
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