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Human SOX10 Antibody

Catalog #: AF2864
51 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2864
AF2864-SP
SOX10 in SK‑Mel‑28 Human Cell Line.
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Product Details
Citations (51)
FAQs
Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX10 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SOX10
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human melanoma tissue
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

SOX10 in SK‑Mel‑28 Human Cell Line. SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry SOX10 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

Long Name
Transcription Factor SOX10
Entrez Gene IDs
6663 (Human)
Alternate Names
DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of

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Citations for Human SOX10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

51 Citations: Showing 1 - 10
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  1. Serum amyloid A3 fuels a feed-forward inflammatory response to the bacterial amyloid curli in the enteric nervous system
    Authors: Verstraelen, P;Van Remoortel, S;De Loose, N;Verboven, R;Garcia-Diaz Barriga, G;Christmann, A;Gries, M;Bessho, S;Li, J;Guerra, C;Tükel, Ç;Ibiza Martinez, S;Schäfer, KH;Timmermans, JP;De Vos, WH;
    Cellular and molecular gastroenterology and hepatology
    Species: Human hepegivirus
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. A single nuclear transcriptomic characterisation of mechanisms responsible for impaired angiogenesis and blood-brain barrier function in Alzheimer's disease
    Authors: Tsartsalis, S;Sleven, H;Fancy, N;Wessely, F;Smith, AM;Willumsen, N;Cheung, TKD;Rokicki, MJ;Chau, V;Ifie, E;Khozoie, C;Ansorge, O;Yang, X;Jenkyns, MH;Davey, K;McGarry, A;Muirhead, RCJ;Debette, S;Jackson, JS;Montagne, A;Owen, DR;Miners, JS;Love, S;Webber, C;Cader, MZ;Matthews, PM;
    Nature communications
    Species: Human hepegivirus
    Sample Types: Nuclei
    Applications: Flow Cytometry
  3. A Novel Ex Vivo Model to Study Therapeutic Treatments for Myelin Repair following Ischemic Damage
    Authors: Werner, L;Gliem, M;Rychlik, N;Pavic, G;Reiche, L;Kirchhoff, F;Silva Oliveira Junior, M;Gruchot, J;Meuth, SG;Küry, P;Göttle, P;
    International journal of molecular sciences
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. SARM1 detection in myelinating glia: sarm1/Sarm1 is dispensable for PNS and CNS myelination in zebrafish and mice
    Authors: SV Fazal, C Mutschler, CZ Chen, M Turmaine, CY Chen, YP Hsueh, A Ibañez-Gra, A Loreto, A Casillas-B, H Cabedo, RJM Franklin, RA Barker, KR Monk, BJ Steventon, MP Coleman, JA Gomez-Sanc, P Arthur-Far
    Frontiers in Cellular Neuroscience, 2023-04-05;17(0):1158388.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: ICC
  5. Single-Cell Characterization of the Frizzled 5 (Fz5) Mutant Mouse and Human Persistent Fetal Vasculature (PFV)
    Authors: Y Chen, C Wu, S Peng, D Guo, H Ouyang, Y Wei, R Ju, X Ding, Z Xie, C Liu
    Investigative Ophthalmology & Visual Science, 2023-03-01;64(3):8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Pervasive environmental chemicals impair oligodendrocyte development
    Authors: EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar
    bioRxiv : the preprint server for biology, 2023-02-12;0(0):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  7. Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress
    Authors: Z Li, K Chen, Q Shao, H Lu, X Zhang, Y Pu, X Sun, H He, L Cao
    Journal of Neuroinflammation, 2023-01-30;20(1):16.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  8. Enhanced proliferation of oligodendrocyte progenitor cells following retrovirus mediated Achaete-scute complex-like 1 overexpression in the postnatal cerebral cortex in vivo
    Authors: C Galante, N Marichal, FF Scarante, LM Ghayad, Y Shi, C Schuurmans, B Berninger, S Péron
    Frontiers in Neuroscience, 2022-12-02;16(0):919462.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. A cellular hierarchy in melanoma uncouples growth and metastasis
    Authors: P Karras, I Bordeu, J Pozniak, A Nowosad, C Pazzi, N Van Raemdo, E Landeloos, Y Van Herck, D Pedri, G Bervoets, S Makhzami, JH Khoo, B Pavie, J Lamote, O Marin-Beja, M Dewaele, H Liang, X Zhang, Y Hua, J Wouters, R Browaeys, G Bergers, Y Saeys, F Bosisio, J van den Oo, D Lambrechts, AK Rustgi, O Bechter, C Blanpain, BD Simons, F Rambow, JC Marine
    Nature, 2022-09-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC/IF
  10. Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms.
    Authors: Ideno H, Imaizumi K, Shimada H, Sanosaka T, Nemoto A, Kohyama J, Okano H
    iScience, 2022-09-16;25(10):105140.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  11. Identification and quantification of nociceptive Schwann cells in mice with and without Streptozotocin-induced diabetes
    Authors: X Hu, N Agarwal, MD Zhang, P Ernfors, R Kuner, JR Nyengaard, P Karlsson
    Journal of chemical neuroanatomy, 2022-06-06;0(0):102118.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Serotonin limits generation of chromaffin cells during adrenal organ development
    Authors: P Kameneva, VI Melnikova, ME Kastriti, A Kurtova, E Kryukov, A Murtazina, L Faure, I Poverennay, AV Artemov, TS Kalinina, NV Kudryashov, M Bader, J Skoda, P Chlapek, L Curylova, L Sourada, J Neradil, M Tesarova, M Pasqualett, P Gaspar, VD Yakushov, BI Sheftel, T Zikmund, J Kaiser, K Fried, N Alenina, EE Voronezhsk, I Adameyko
    Nature Communications, 2022-05-25;13(1):2901.
    Species: Rat
    Sample Types: Embryos
    Applications: IHC
  13. Role of YAP in early ectodermal specification and a Huntington's Disease model of human neurulation
    Authors: FM Piccolo, NR Kastan, T Haremaki, Q Tian, TL Laundos, R De Santis, AJ Beaudoin, TS Carroll, JD Luo, K Gnedeva, F Etoc, AJ Hudspeth, AH Brivanlou
    Elife, 2022-04-22;11(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  14. G-quadruplex DNA structures in human stem cells and differentiation
    Authors: KG Zyner, A Simeone, SM Flynn, C Doyle, G Marsico, S Adhikari, G Portella, D Tannahill, S Balasubram
    Nature Communications, 2022-01-10;13(1):142.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Nutritional regulation of oligodendrocyte differentiation regulates perineuronal net remodeling in the median eminence
    Authors: S Kohnke, S Buller, D Nuzzaci, K Ridley, B Lam, H Pivonkova, MA Bentsen, KM Alonge, C Zhao, J Tadross, S Holmqvist, T Shimizo, H Hathaway, H Li, W Macklin, MW Schwartz, WD Richardson, GSH Yeo, RJM Franklin, RT Karadottir, DH Rowitch, C Blouet
    Cell Reports, 2021-07-13;36(2):109362.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. H3K27 demethylases are dispensable for activation of Polycomb-regulated injury response genes in peripheral nerve
    Authors: P Duong, KH Ma, R Ramesh, JJ Moran, S Won, J Svaren
    The Journal of Biological Chemistry, 2021-06-04;297(1):100852.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Donor cell memory confers a metastable state of directly converted cells
    Authors: KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler
    Cell Stem Cell, 2021-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Single-cell transcriptomics of human embryos identifies multiple sympathoblast lineages with potential implications for neuroblastoma origin
    Authors: P Kameneva, AV Artemov, ME Kastriti, L Faure, TK Olsen, J Otte, A Erickson, B Semsch, ER Andersson, M Ratz, J Frisén, AS Tischler, RR de Krijger, T Bouderliqu, N Akkuratova, M Vorontsova, O Gusev, K Fried, E Sundström, S Mei, P Kogner, N Baryawno, PV Kharchenko, I Adameyko
    Nature Genetics, 2021-04-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. TDP-43 maximizes nerve conduction velocity by repressing a cryptic exon for paranodal junction assembly in Schwann cells
    Authors: KJ Chang, I Agrawal, A Vainshtein, WY Ho, W Xin, G Tucker-Kel, K Susuki, E Peles, SC Ling, JR Chan
    Elife, 2021-03-10;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
    Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
    Nature Neuroscience, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons
    Authors: AJ Hulme, JR McArthur, S Maksour, S Miellet, L Ooi, DJ Adams, RK Finol-Urda, M Dottori
    Frontiers in Cellular Neuroscience, 2020-12-04;14(0):600895.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells
    Authors: JA García-Leó, B García-Día, K Eggermont, L Cáceres-Pa, K Neyrinck, R Madeiro da, JC Dávila, A Baron-Van, A Gutiérrez, CM Verfaillie
    Nat Protoc, 2020-10-23;15(11):3716-3744.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function
    Authors: KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar
    Cell Stem Cell, 2020-10-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Interneuron hypomyelination is associated with cognitive inflexibility in a rat model of schizophrenia
    Authors: DA Maas, VD Eijsink, M Spoelder, JA van Hulten, P De Weerd, JR Homberg, A Vallès, B Nait-Oumes, GJM Martens
    Nat Commun, 2020-05-11;11(1):2329.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  25. Single-nucleus transcriptomics of the prefrontal cortex in major depressive disorder implicates oligodendrocyte precursor cells and excitatory neurons
    Authors: C Nagy, M Maitra, A Tanti, M Suderman, JF Théroux, MA Davoli, K Perlman, V Yerko, YC Wang, SJ Tripathy, P Pavlidis, N Mechawar, J Ragoussis, G Turecki
    Nat. Neurosci., 2020-04-27;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ICC
  26. The Role of Transthyretin in Oligodendrocyte Development
    Authors: B Alshehri, M Pagnin, JY Lee, S Petratos, SJ Richardson
    Sci Rep, 2020-03-06;10(1):4189.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Oligodendrocyte Intrinsic miR-27a Controls Myelination and Remyelination
    Authors: A Tripathi, C Volsko, JP Garcia, E Agirre, KC Allan, PJ Tesar, BD Trapp, G Castelo-Br, FJ Sim, R Dutta
    Cell Rep, 2019-10-22;29(4):904-919.e9.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Perturbed development of cranial neural crest cells in association with reduced sonic hedgehog signaling underlies the pathogenesis of retinoic-acid-induced cleft palate
    Authors: Q Wang, H Kurosaka, M Kikuchi, A Nakaya, PA Trainor, T Yamashiro
    Dis Model Mech, 2019-10-04;12(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
    Authors: S Wiszniak, Q Schwarz
    BMC Neurosci, 2019-04-29;20(1):21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  30. Dynamics of oligodendrocyte generation in multiple sclerosis
    Authors: MSY Yeung, M Djelloul, E Steiner, S Bernard, M Salehpour, G Possnert, L Brundin, J Frisén
    Nature, 2019-01-23;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. A unique role for DNA (hydroxy)methylation in epigenetic regulation of human inhibitory neurons
    Authors: A Kozlenkov, J Li, P Apontes, YL Hurd, WM Byne, EV Koonin, M Wegner, EA Mukamel, S Dracheva
    Sci Adv, 2018-09-26;4(9):eaau6190.
    Species: Human
    Sample Types: Nuclei
    Applications: FACS
  32. Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
    Authors: AM Lager, OG Corradin, JM Cregg, MS Elitt, HE Shick, BLL Clayton, KC Allan, HE Olsen, M Madhavan, PJ Tesar
    Nat Commun, 2018-09-13;9(1):3708.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  33. Intracellular attenuation of BMP signaling via CKIP-1/Smurf1 is essential during neural crest induction
    Authors: ML Piacentino, ME Bronner
    PLoS Biol., 2018-06-27;16(6):e2004425.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. The Dorsal Wave of Neocortical Oligodendrogenesis Begins Embryonically and Requires Multiple Sources of Sonic Hedgehog
    Authors: CC Winkler, OR Yabut, SP Fregoso, HG Gomez, BE Dwyer, SJ Pleasure, SJ Franco
    J. Neurosci., 2018-05-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. ASCL1 regulates proliferation of NG2-glia in the embryonic and adult spinal cord
    Authors: DP Kelenis, E Hart, M Edwards-Fl, JE Johnson, TY Vue
    Glia, 2018-04-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Exposure of human melanocytes to UVB twice and subsequent incubation leads to cellular senescence and senescence-associated pigmentation through the prolonged p53 expression
    Authors: SY Choi, BH Bin, W Kim, E Lee, TR Lee, EG Cho
    J. Dermatol. Sci., 2018-03-02;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Central and Peripheral Nervous System Progenitors Derived from Human Pluripotent Stem Cells Reveal a Unique Temporal and Cell-Type Specific Expression of PMCAs
    Authors: M Chen, SH Laursen, M Habekost, CH Knudsen, SH Buchholdt, J Huang, F Xu, X Liu, L Bolund, Y Luo, P Nissen, F Febbraro, M Denham
    Front Cell Dev Biol, 2018-02-06;6(0):5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem�Cells
    Authors: KD Abu-Bonsra, D Zhang, AR Bjorksten, M Dottori, DF Newgreen
    Stem Cell Reports, 2017-12-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. Graded elevation of c-Jun in Schwann cells in vivo: gene dosage determines effects on development, re-myelination, tumorigenesis and hypomyelination
    Authors: SV Fazal, JA Gomez-Sanc, LJ Wagstaff, N Musner, G Otto, M Janz, R Mirsky, KR Jessen
    J. Neurosci., 2017-11-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. SOX10 Expression as Well as BRAF and GNAQ/11 Mutations Distinguish Pigmented Ciliary Epithelium Neoplasms From Uveal Melanomas
    Authors: T Mori, A Sukeda, S Sekine, S Shibata, E Ryo, H Okano, S Suzuki, N Hiraoka
    Invest. Ophthalmol. Vis. Sci., 2017-10-01;58(12):5445-5451.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  41. Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system
    Authors: G Figlia, C Norrmén, JA Pereira, D Gerber, U Suter
    Elife, 2017-09-07;6(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
    Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
    Am. J. Hum. Genet., 2017-03-30;100(4):617-634.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Analysis of induced pluripotent stem cells carrying 22q11.2 deletion.
    Authors: T Yoshikawa, Hisano Y, Iwayama Y, Toyota T, Itasaka N, Dean B
    Transl Psychiatry, 2016-11-01;6(11):e934.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells
    Authors: Yannick Poitelon
    Nat Neurosci, 2016-06-06;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  45. Elevated Levels of SOX10 in Serum from Vitiligo and Melanoma Patients, Analyzed by Proximity Ligation Assay
    Authors: A Blokzijl, LE Chen, SM Gustafsdot, J Vuu, G Ullenhag, O Kämpe, U Landegren, M Kamali-Mog, H Hedstrand
    PLoS ONE, 2016-04-25;11(4):e0154214.
    Species: Human
    Sample Types: Serum
    Applications: Proximity Ligation Assay (PLA)
  46. Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo.
    Authors: Najm F, Madhavan M, Zaremba A, Shick E, Karl R, Factor D, Miller T, Nevin Z, Kantor C, Sargent A, Quick K, Schlatzer D, Tang H, Papoian R, Brimacombe K, Shen M, Boxer M, Jadhav A, Robinson A, Podojil J, Miller S, Miller R, Tesar P
    Nature, 2015-04-20;522(7555):216-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  47. Double minute amplification of mutant PDGF receptor alpha in a mouse glioma model.
    Authors: Zou H, Feng R, Huang Y, Tripodi J, Najfeld V, Tsankova N, Jahanshahi M, Olson L, Soriano P, Friedel R
    Sci Rep, 2015-02-16;5(0):8468.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Dynamic regulation of Schwann cell enhancers after peripheral nerve injury.
    Authors: Hung H, Sun G, Keles S, Svaren J
    J Biol Chem, 2015-01-22;290(11):6937-50.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. Migrating cells mediate long-range WNT signaling.
    Authors: Serralbo, Olivier, Marcelle, Christop
    Development, 2014-05-01;141(10):2057-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  50. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013-04-14;31(5):426-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  51. Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad.
    Authors: Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H
    Cell Stem Cell, 2008-04-10;2(0):392-403.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human SOX10 Antibody

Average Rating: 4.3 (Based on 7 Reviews)

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Human SOX10 Antibody
By Anonymous on 02/06/2020
Application: ICC/IF Sample Tested: iPS2 human induced pluripotent stem cells Species: human iPS cells

Generated cells of oligodendrocytes lineage from iPS cells

Immunocytochemistry/Immunofluorescence Human SOX10 Antibody AF2864
Human SOX10 Antibody
By Anonymous on 10/29/2019
Application: ICC/IF Sample Tested: Adult brain Species: Mouse
Immunocytochemistry/Immunofluorescence Human SOX10 Antibody AF2864
Human SOX10 Antibody
By Jennifer Taggart on 08/20/2018
Application: ICC/IF Sample Tested: Neural progenitor cells Species: Human
Human SOX10 Antibody
By shelley Cutrone on 08/20/2018
Application: ICC/IF Sample Tested: Neural progenitor cells Species: Human
Human SOX10 Antibody
By Anonymous on 06/20/2018
Application: IHC Sample Tested: Adult brain Species: Mouse

This antibody worked beautifully at 1:1000 in adult (P25) mouse brain perfused with 4% paraformaldehyde, 30um free floating sections.
1. Antigen Retrieval: 100mM Sodium Citrate pH6.0, 0.05%
2. Rinse sections PBS once
3. Block in 5% NDS in PBSTx 1% RT for 1 hour
4. Primary Antibody (1:1000) in 1% NDS in PBSTx 0.3% (Incubate RT overnight on shaker)
5. Wash 3x 10 min PBS
6. Secondary Antibody 1:600 in 1% NDS in PBSTx 0.3% (Incubate RT 2h on shaker)
7. Wash 3x 10 min PBS
8. Mount on slides in water and let dry
9. Coverslip using Prolong Gold

Immunohistochemistry Human SOX10 Antibody AF2864
Human SOX10 Antibody
By Venkatkrishnan Sundaram on 11/02/2017
Application: IHC Sample Tested: Sciatic nerve Species: Mouse

Cryosection of mouse sciatic nerve 10µm thick. Tissue fixed with 4% PFA after dissection, inclusion in OCT. Storage at -80°C.

Thawed sections at RT are treated with TritonX100 0.1% Tween 20 0.1% in PBS 1X for half an hour before blocking and primary antibody.

Immunohistochemistry Human SOX10 Antibody AF2864
Human SOX10 Antibody
By luciana frick on 07/22/2017
Application: ICC/IF Sample Tested: sciatic nerve culture Species: Mouse

Cell culture from mouse sciatic nerves, Sox10+ cells are Schwann cells. Standard immunostaining, 5ug/ml of goat anti sox10 antibody. Dapi counterstaining.

Immunocytochemistry/Immunofluorescence Human SOX10 Antibody AF2864