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Human Total Annexin A2 DuoSet IC ELISA

Catalog #: DYC3928-2
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC3928E
DYC3928-5
DYC3928-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Product Details
Citations (1)
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Total Annexin A2 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
312.5 - 20,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure Annexin A2 in cell lysates. An immobilized capture antibody specific for Annexin A2 binds both phosphorylated and unphosphorylated Annexin A2. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Annexin A2

Annexins are a multigene family of Ca++-regulated phospholipid-binding proteins. They serve as membrane-membrane, or membrane-cytoskeleton linkers, and regulate the formation of lipid domains in the plasma membrane. The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments.

Entrez Gene IDs:
302 (Human); 12306 (Mouse); 56611 (Rat)
Alternate Names:
Annexin A2; Annexin II; annexin-2; ANX2; ANX2L4; ANX2L4LPC2; ANXA2; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; chromobindin-8; LIP2; LIP2PAP-IV; Lipocortin II; Lipocortin-2; LPC2D; LPC2DP36; p36; PAP-IV; Placental anticoagulant protein IV; Protein I

Citation for Human Total Annexin A2 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Clinical Significance of Annexin A2 Expression in Breast Cancer Patients
    Authors: LD Gibbs, K Mansheim, S Maji, R Nandy, CM Lewis, JK Vishwanath, P Chaudhary
    Cancers, 2020-12-22;13(1):.
    Species: Human
    Sample Types: Serum

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