Mouse Adiponectin/Acrp30 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).
Blocking Buffer: 1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
Scientific Data
Product Datasheets
Preparation and Storage
Background: Adiponectin/Acrp30
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.
Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Assay Procedure
- Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Mouse Adiponectin/Acrp30 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Diet switch pre-vaccination improves immune response and metabolic status in formerly obese mice
Authors: Honce, R;Vazquez-Pagan, A;Livingston, B;Mandarano, AH;Wilander, BA;Cherry, S;Hargest, V;Sharp, B;Brigleb, PH;Kirkpatrick Roubidoux, E;Van de Velde, LA;Skinner, RC;McGargill, MA;Thomas, PG;Schultz-Cherry, S;
Nature microbiology
Species: Murine polyomavirus strain A3
Sample Types: Serum
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Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice
Authors: TS Huang, T Wu, YD Wu, XH Li, J Tan, CH Shen, SJ Xiong, ZQ Feng, SF Gao, H Li, WB Cai
Nature Communications, 2023-01-24;14(1):390.
Species: Mouse
Sample Types: Serum
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Paeoniflorin, a constituent of Kami-shoyo-san, suppresses blood glucose levels in postmenopausal diabetic mice by promoting the secretion of estradiol from adipocytes
Authors: K Kobayashi, YT Tang, K Sasaki
Oncogene, 2022-09-05;32(0):101335.
Species: Mouse
Sample Types: Cell Culture Supernates
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Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling
Authors: L Orliaguet, T Ejlalmanes, A Humbert, R Ballaire, M Diedisheim, JB Julla, D Chokr, J Cuenco, J Michielett, J Charbit, D Lindén, J Boucher, C Potier, A Hamimi, S Lemoine, C Blugeon, P Legoix, S Lameiras, LG Baudrin, S Baulande, A Soprani, FA Castelli, F Fenaille, JP Riveline, E Dalmas, J Rieusset, JF Gautier, N Venteclef, F Alzaid
Nature Communications, 2022-08-30;13(1):5089.
Species: Mouse
Sample Types: Plasma
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Toll-like Receptor 7 (TLR7) Is Expressed in Adipocytes and the Pharmacological TLR7 Agonist Imiquimod and Adipocyte-Derived Cell-Free Nucleic Acids (cfDNA) Regulate Adipocyte Function
Authors: M Thomalla, A Schmid, J Hehner, S Koehler, E Neumann, U Müller-Lad, A Schäffler, T Karrasch
International Journal of Molecular Sciences, 2022-07-30;23(15):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Adiponectin Deficiency Alters Placenta Function but Does Not Affect Fetal Growth in Mice
Authors: M Mohan Shre, S Wermelin, E Stener-Vic, I Wernstedt, A Benrick
International Journal of Molecular Sciences, 2022-04-29;23(9):.
Species: Mouse
Sample Types: Serum
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Effects of High-Fat and High-Fat/High-Sucrose Diet-Induced Obesity on PVAT Modulation of Vascular Function in Male and Female Mice
Authors: JA Victorio, DM Guizoni, IN Freitas, TR Araujo, AP Davel
Frontiers in Pharmacology, 2021-09-10;12(0):720224.
Species: Mouse
Sample Types: Serum
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Effect of fabrication parameters on morphology and drug loading of polymer particles for rosiglitazone delivery
Authors: MR Spetz, C Isely, RM Gower
Journal of drug delivery science and technology, 2021-07-01;65(1):.
Species: Mouse
Sample Types: Cell Culture Supernates
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The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding
Authors: K Kuramoto, YJ Kim, JH Hong, C He
Cell Reports, 2021-05-25;35(8):109184.
Species: Mouse
Sample Types: Serum
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Fish Oil Enriched in EPA, but Not in DHA, Reverses the Metabolic Syndrome and Adipocyte Dysfunction Induced by a High-Fat Diet
Authors: RDC da Cunha d, JJ Simão, VSD Silva, TM Farias, MM Cruz, VJ Antraco, L Armelin-Co, MI Alonso-Val
Nutrients, 2021-02-26;13(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
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C1q/TNF-Related Protein 3 (CTRP-3) Deficiency of Adipocytes Affects White Adipose Tissue Mass but Not Systemic CTRP-3 Concentrations
Authors: A Schmid, M Roderfeld, J Gehl, E Roeb, A Nist, HR Chung, T Stiewe, T Karrasch, A Schäffler
International Journal of Molecular Sciences, 2021-02-07;22(4):.
Species: Mouse
Sample Types: Serum
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SR-BI deficiency disassociates obesity from hepatic steatosis and glucose intolerance development in high fat diet-fed mice
Authors: M Hoekstra, AB Ouweneel, J Price, R van der Ge, RJ van der Sl, JJ Geerling, JE Nahon, M Van Eck
The Journal of nutritional biochemistry, 2020-12-13;89(0):108564.
Species: Mouse
Sample Types: Plasma
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Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds
Authors: EM Ahn, G Asamenew, HW Kim, SH Lee, SM Yoo, SM Cho, YS Cha, MS Kang
Nutrients, 2020-04-29;12(5):.
Species: Mouse
Sample Types: Cell Culture Supernates
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HILPDA Uncouples Lipid Droplet Accumulation in Adipose Tissue Macrophages from Inflammation and Metabolic Dysregulation
Authors: XAMH van Dieren, MA de la Rosa, A Georgiadi, F Mattijssen, W Dijk, M van Weeghe, R Singh, JW Borst, R Stienstra, S Kersten
Cell Rep, 2020-02-11;30(6):1811-1822.e6.
Species: Mouse
Sample Types: Plasma
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Fish oil reverses metabolic syndrome, adipocyte dysfunction, and altered adipokines secretion triggered by high-fat diet-induced obesity
Authors: RDC da Cunha d, MM Cruz, TM de Farias, VS da Silva, J de Jesus S, MM Telles, MIC Alonso-Val
Physiol Rep, 2020-02-01;8(4):e14380.
Species: Mouse
Sample Types: Cell Culture Supernates
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The role of uncoupling protein 2 in macrophages and its impact on obesity-induced adipose tissue inflammation and insulin resistance
Authors: XAMH van Dieren, T Sancerni, MC Alves-Guer, R Stienstra
J Biol Chem, 2020-01-01;0(0):.
Species: Mouse
Sample Types: Plasma
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SOCS2 modulates adipose tissue inflammation and expansion in mice
Authors: CH Val, MC de Oliveir, DR Lacerda, A Barroso, NV Batista, Z Menezes-Ga, DRR de Assis, AT Cramer, F Brant, MM Teixeira, D Glória Sou, AM Ferreira, FS Machado
J. Nutr. Biochem., 2019-11-23;76(0):108304.
Species: Mouse
Sample Types: Serum
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Environmental enrichment improves metabolic and behavioral health in the BTBR mouse model of autism
Authors: NJ Queen, AA Boardman, RS Patel, JJ Siu, X Mo, L Cao
Psychoneuroendocrinology, 2019-10-15;111(0):104476.
Species: Mouse
Sample Types: Serum
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Melatonin Supplementation Attenuates the Pro-Inflammatory Adipokines Expression in Visceral Fat from Obese Mice Induced by A High-Fat Diet
Authors: TDSM Farias, RID Paixao, MM Cruz, RDCDC de Sa, JJ Simão, VJ Antraco, MIC Alonso-Val
Cells, 2019-09-06;8(9):.
Species: Mouse
Sample Types: Tissue Lysates
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Adrenergic stimulation of adiponectin secretion in visceral mouse adipocytes is blunted in high-fat diet induced obesity
Authors: S Musovic, CS Olofsson
Sci Rep, 2019-07-23;9(1):10680.
Species: Mouse
Sample Types: Cell Homogenates
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Exercise-induced AMPK activation and IL-6 muscle production are disturbed in adiponectin knockout mice
Authors: TA Diniz, JCJ Aquino Jún, FC Mosele, C Cabral-San, EA Lima Junio, AAS Teixeira, FS Lira, JC Rosa Neto
Cytokine, 2019-03-20;119(0):71-80.
Species: Mouse
Sample Types: Tissue Homogenates
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Adiponectin deficiency has no effect in murine autoimmune myocarditis
Authors: V Stroikova, A Fischer, M Bockstahle, AM Müller, HA Katus, Z Kaya
Cytokine, 2019-02-01;116(0):139-149.
Species: Mouse
Sample Types: Serum
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A SEPT1-based scaffold is required for Golgi integrity and function
Authors: K Song, C Gras, G Capin, N Gimber, M Lehmann, S Mohd, D Puchkov, M Rödiger, I Wilhelmi, O Daumke, J Schmoranze, A Schürmann, M Krauss
J. Cell. Sci., 2019-02-01;132(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
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GPS2 Deficiency Triggers Maladaptive White Adipose Tissue Expansion in Obesity via HIF1A Activation
Authors: K Drareni, R Ballaire, S Barilla, MJ Mathew, A Toubal, R Fan, N Liang, C Chollet, Z Huang, M Kondili, F Foufelle, A Soprani, R Roussel, JF Gautier, F Alzaid, E Treuter, N Venteclef
Cell Rep, 2018-09-11;24(11):2957-2971.e6.
Species: Mouse
Sample Types: Whole Blood
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Metformin Mitigates Fibrosis and Glucose Intolerance Induced by Doxorubicin in Subcutaneous Adipose Tissue
Authors: LA Biondo, HA Batatinha, CO Souza, AAS Teixeira, LS Silveira, MI Alonso-Val, LM Oyama, MJ Alves, M Seelaender, JCR Neto
Front Pharmacol, 2018-05-08;9(0):452.
Species: Mouse
Sample Types: Serum
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Collagen overlays can inhibit leptin and adiponectin secretion but not lipid accumulation in adipocytes
Authors: SL Christian, NK Pallegar, RJ Brown, AM Viloria-Pe
PeerJ, 2018-04-27;6(0):e4641.
Species: Mouse
Sample Types: Cell Culture Supernates
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Acute physical exercise increases the adaptor protein APPL1 in the hypothalamus of obese mice
Authors: RC Gaspar, VR Muñoz, GP Formigari, GK Kuga, SCBR Nakandakar, JD Botezelli, ASR da Silva, DE Cintra, LP de Moura, ER Ropelle, JR Pauli
Cytokine, 2018-04-26;110(0):87-93.
Species: Mouse
Sample Types: Serum
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Increased Ifi202b/IFI16 expression stimulates adipogenesis in mice and humans
Authors: M Stadion, K Schwerbel, A Graja, C Baumeier, M Rödiger, W Jonas, C Wolfrum, H Staiger, A Fritsche, HU Häring, N Klöting, M Blüher, P Fischer-Po, TJ Schulz, HG Joost, H Vogel, A Schürmann
Diabetologia, 2018-02-24;0(0):.
Species: Mouse
Sample Types: Plasma
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Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes
Authors: M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta, A Schürmann
Mol Metab, 2017-11-22;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Egg white hydrolysate shows insulin mimetic and sensitizing effects in 3T3-F442A pre-adipocytes
Authors: F Jahandideh, S Chakrabart, ST Davidge, J Wu
PLoS ONE, 2017-10-03;12(10):e0185653.
Species: Mouse
Sample Types: Cell Culture Supernates
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Mathematical modeling of white adipocyte exocytosis predicts adiponectin secretion and quantifies the rate of vesicle exo- and endocytosis
Authors: C Brännmark, W Lövfors, AM Komai, T Axelsson, MF El Hachman, S Musovic, A Paul, E Nyman, CS Olofsson
J. Biol. Chem., 2017-09-25;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Adipose tissue, metabolic and inflammatory responses to stroke are altered in obese mice
Authors: MJ Haley, G Mullard, KA Hollywood, GJ Cooper, WB Dunn, CB Lawrence
Dis Model Mech, 2017-08-10;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Inducing maternal inflammation promotes leptin production in offspring but does not improve allergic symptoms in a mouse model of allergic rhinitis
Authors: A Imai, K Satoi, E Fujimoto, K Sato
Heliyon, 2017-06-22;3(6):e00327.
Species: Mouse
Sample Types: Serum
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Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in�vitro
Authors: A Aoki-Yoshi, S Saito, T Tsuruta, A Ohsumi, H Tsunoda, K Sonoyama
Biochem. Biophys. Res. Commun., 2017-05-27;0(0):.
Species: Mouse
Sample Types: Serum
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Palmitoleic Acid Improves Metabolic Functions in Fatty Liver by PPAR?-Dependent AMPK Activation
Authors: Camila O Souza
J. Cell. Physiol, 2017-03-24;0(0):.
Species: Mouse
Sample Types: Serum
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Obesity-induced diet leads to weight gain, systemic metabolic alterations, adipose tissue inflammation, hepatic steatosis, and oxidative stress in gerbils (Meriones unguiculatus)
Authors: LL Ventura, NC Fortes, HC Santiago, MV Caliari, MA Gomes, DR Oliveira
PeerJ, 2017-03-02;5(0):e2967.
Species: Gerbil
Sample Types: Tissue Homogenates
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Hepatic Expression of Adenovirus 36 E4ORF1 Improves Glycemic Control and Promotes Glucose Metabolism via AKT Activation
Authors: Travis B McMurphy
Diabetes, 2016-11-30;0(0):.
Species: Mouse
Sample Types: Serum
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Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes
Authors: H Lee, H Li, M Noh, JH Ryu
Int J Mol Sci, 2016-04-08;17(4):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Loss of CD24 in Mice Leads to Metabolic Dysfunctions and a Reduction in White Adipocyte Tissue.
Authors: Fairbridge N, Southall T, Ayre D, Komatsu Y, Raquet P, Brown R, Randell E, Kovacs C, Christian S
PLoS ONE, 2015-11-04;10(11):e0141966.
Species: Mouse
Sample Types: Plasma
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Milk-derived tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) promote adipocyte differentiation and inhibit inflammation in 3T3-F442A cells.
Authors: Chakrabarti S, Wu J
PLoS ONE, 2015-02-25;10(2):e0117492.
Species: Mouse
Sample Types: Cell Culture Supernates
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Obesity increases airway hyperresponsiveness via the TNF-alpha pathway and treating obesity induces recovery.
Authors: Kim J, Sohn J, Lee J, Park J
PLoS ONE, 2015-02-06;10(2):e0116540.
Species: Mouse
Sample Types: Tissue Homogenates
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Interleukin-15 modulates adipose tissue by altering mitochondrial mass and activity.
Authors: Barra, Nicole G, Palanivel, Rengasam, Denou, Emmanuel, Chew, Marianne, Gillgrass, Amy, Walker, Tina D, Kong, Josh, Richards, Carl D, Jordana, Manel, Collins, Stephen, Trigatti, Bernardo, Holloway, Alison C, Raha, Sandeep, Steinberg, Gregory, Ashkar, Ali A
PLoS ONE, 2014-12-17;9(12):e114799.
Species: Mouse
Sample Types: Cell Culture Supernates
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Metabolic profile response to administration of epigallocatechin-3-gallate in high-fat-fed mice.
Authors: Moreno M, De Laquila R, Okuda M, Lira F, de Souza G, de Souza C, Telles M, Ribeiro E, do Nascimento C, Oyama L
Diabetol Metab Syndr, 2014-08-12;6(1):84.
Species: Mouse
Sample Types: Tissue Homogenates
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The adipokine Retnla modulates cholesterol homeostasis in hyperlipidemic mice.
Authors: Lee M, Lim C, Lee Y, Park J, Sonn S, Lee M, Jung I, Jeong S, Jeon S, Lee M, Oh K, Yang Y, Kim J, Choi H, Jeong W, Jeong T, Yoon W, Kim H, Choi J, Oh G
Nat Commun, 2014-07-15;5(0):4410.
Species: Mouse
Sample Types: Serum
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Lactoferrin dampens high-fructose corn syrup-induced hepatic manifestations of the metabolic syndrome in a murine model.
Authors: Li Y, Hsieh C
PLoS ONE, 2014-05-09;9(5):e97341.
Species: Mouse
Sample Types: Tissue Homogenates
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Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice.
Authors: Liu, Xianglan, McMurphy, Travis, Xiao, Run, Slater, Andrew, Huang, Wei, Cao, Lei
Mol Ther, 2014-03-18;22(7):1275-84.
Species: Mouse
Sample Types: Serum
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Increased expression of c-Jun in nonalcoholic fatty liver disease.
Authors: Dorn C, Engelmann J, Saugspier M, Koch A, Hartmann A, Muller M, Spang R, Bosserhoff A, Hellerbrand C
Lab Invest, 2014-02-03;94(4):394-408.
Species: Mouse
Sample Types: Serum
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Lack of platelet-activating factor receptor protects mice against diet-induced adipose inflammation and insulin-resistance despite fat pad expansion.
Authors: Menezes-Garcia Z, Oliveira M, Lima R, Soriani F, Cisalpino D, Botion L, Teixeira M, Souza D, Ferreira A
Obesity (Silver Spring), 2013-12-14;22(3):663-72.
Species: Mouse
Sample Types: Serum
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Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice.
Authors: Gauvreau D, Gupta A, Fisette A, Tom F, Cianflone K
PLoS ONE, 2013-04-22;8(4):e60795.
Species: Mouse
Sample Types: Plasma
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Elevated levels of circulating IL-18BP and perturbed regulation of IL-18 in schizophrenia.
J Neuroinflammation, 2012-08-22;9(0):206.
Species: Human
Sample Types: Serum
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Mutant amyloid precursor protein differentially alters adipose biology under obesogenic and non-obesogenic conditions.
Authors: Freeman, Linnea R, Zhang, Le, Dasuri, Kalavath, Fernandez-Kim, Sun-Ok, Bruce-Keller, Annadora, Keller, Jeffrey
PLoS ONE, 2012-08-17;7(8):e43193.
Species: Mouse
Sample Types: Serum
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Rosiglitazone improves survival and hastens recovery from pancreatic inflammation in obese mice.
Authors: Pini M, Rhodes DH, Castellanos KJ
PLoS ONE, 2012-07-16;7(7):e40944.
Species: Mouse
Sample Types: Plasma
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Effect of secretin on preadipocyte, differentiating and mature adipocyte functions.
Authors: Miegueu P, Cianflone K, Richard D, St-Pierre D
Int J Obes (Lond), 2012-05-08;37(3):366-74.
Species: Mouse
Sample Types: Cell Culture Supernates
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