Mouse BAMBI/NMA Antibody

Name: Mouse BAMBI/NMA Antibody
Brand: R&D Systems
SKU: AF2387
Rating: 4.3 (3 reviews)

Catalog #: AF2387
3 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2387
AF2387-SP

Detection of Mouse BAMBI/NMA by Western Blot.

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All BAMBI/NMA Antibodies

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Citations (3)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BAMBI/NMA Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BAMBI/NMA in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human BAMBI is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BAMBI/NMA
Ile28-Thr143
Accession # Q9D0L6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse BAMBI/NMA by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAMBI/NMA

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Long Name

BMP and Activin Membrane-bound Inhibitor

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Alternate Names

BAMBI; BMP and activin membrane-bound inhibitor homolog (Xenopus laevis); BMP and activin membrane-bound inhibitor homolog; NMA; NMANon-metastatic gene A protein; Putative transmembrane protein NMA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse BAMBI/NMA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010-09-24;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation.
Authors: Tramullas M, Lantero A, Diaz A, Morchon N, Merino D, Villar A, Buscher D, Merino R, Hurle JM, Izpisua-Belmonte JC, Hurle MA
J. Neurosci., 2010-01-27;30(4):1502-11.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
TLR4 enhances TGF-beta signaling and hepatic fibrosis.
Authors: Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF
Nat. Med., 2007-10-21;13(11):1324-32.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot