Mouse CD25/IL-2R alpha DuoSet ELISA
Mouse CD25/IL-2R alpha DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: CD25/IL-2R alpha
CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Mouse CD25/IL-2R alpha DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Adoptive T cell therapy cures mice from active hemophagocytic lymphohistiocytosis (HLH)
Authors: K Wei beta ert, S Ammann, T Kögl, V Dettmer-Mo, C Schell, T Cathomen, S Ehl, P Aichele
Embo Molecular Medicine, 2022-10-24;0(0):e16085.
Species: Mouse
Sample Types: Serum
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Familial hemophagocytic lymphohistiocytosis hepatitis is mediated by IFN-gamma in a predominantly hepatic-intrinsic manner
Authors: T Diamond, TN Burn, MA Nishiguchi, D Minichino, J Chase, N Chu, PA Kreiger, EM Behrens
PLoS ONE, 2022-06-07;17(6):e0269553.
Species: Mouse
Sample Types: Serum
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The metalloprotease ADAM10 generates soluble interleukin-2 receptor alpha (sIL-2Ralpha/sCD25) in vivo
Authors: S Kirschke, I Ogunsulire, B Selvakumar, N Schumacher, T Sezin, S Rose-John, A Scheffold, C Garbers, J Lokau
The Journal of Biological Chemistry, 2022-04-07;0(0):101910.
Species: Mouse
Sample Types: Serum
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Trigger-dependent differences determine therapeutic outcome in murine primary hemophagocytic lymphohistiocytosis
Authors: R Gather, P Aichele, N Goos, J Rohr, H Pircher, T Kögl, R Zeiser, H Hengel, A Schmitt-Gr, C Weaver, S Ehl
Eur. J. Immunol., 2020-06-02;0(0):.
Species: Mouse
Sample Types: Serum
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A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation
Authors: SJ Tavernier, V Athanasopo, P Verloo, G Behrens, J Staal, DJ Bogaert, L Naesens, M De Bruyne, S Van Gassen, E Parthoens, J Ellyard, J Cappello, LX Morris, H Van Gorp, G Van Isterd, Y Saeys, M Lamkanfi, P Schelstrae, J Dehoorne, V Bordon, R Van Coster, BN Lambrecht, B Menten, R Beyaert, CG Vinuesa, V Heissmeyer, M Dullaers, F Haerynck
Nat Commun, 2019-10-21;10(1):4779.
Species: Mouse
Sample Types: Serum
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EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells
Nature, 2016-05-05;533(7601):110-4.
Species: Mouse
Sample Types: Cell Culture Supernates
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Subtle differences in CTL cytotoxicity determine susceptibility to hemophagocytic lymphohistiocytosis in mice and humans with Chediak-Higashi syndrome.
Authors: Jessen B, Maul-Pavicic A, Ufheil H, Vraetz T, Enders A, Lehmberg K, Langler A, Gross-Wieltsch U, Bay A, Kaya Z, Bryceson YT, Koscielniak E, Badawy S, Davies G, Hufnagel M, Schmitt-Graeff A, Aichele P, Zur Stadt U, Schwarz K, Ehl S
Blood, 2011-08-30;118(17):4620-9.
Species: Mouse
Sample Types: Serum
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Bromelain treatment reduces CD25 expression on activated CD4+ T cells in vitro.
Authors: Secor ER, Singh A, Guernsey LA
Int. Immunopharmacol., 2009-01-20;9(3):340-6.
Species: Mouse
Sample Types: Cell Culture Supernates
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