Mouse GFR alpha -like Antibody Summary
Gln20-Gly349
Accession # Q6SJE0-1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GFR alpha ‑like in HEK293 Human Cell Line Transfected. GFR alpha ‑like was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rat Anti-Mouse GFR alpha ‑like Monoclonal Antibody (Catalog # MAB5728) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-like
GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.
- Li, Z. et al. (2005) J. Neurochem. 95:361.
- Yang, L. et al. (2017) Nature Medicine 23:1158.
- Mullican, S. et al. (2017) Nature medicine 23:1150.
Emmerson, P. et al. (2017) Nature Medicine 23:1215.
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