Mouse NGFR/TNFRSF16 Antibody Summary
Gly20-Asn243
Accession # Q9Z0W1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse NGF R/TNFRSF16 by Western Blot. Western blot shows lysates of mouse uterus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for NGF R/TNFRSF16 at approximately 60-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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NGF R/TNFRSF16 in Mouse Brain. NGF R/TNFRSF16 was detected in perfusion fixed frozen sections of mouse brain (cortex) using 7 µg/mL Goat Anti-Mouse NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1157) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NGFR/TNFRSF16
The low affinity nerve growth factor receptor (NGF R), also named p75 neurotrophin receptor, is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family and has been designated TNFRSF16. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region of the receptor contains a subtype 2 death domain.
NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma, and in the amniotic fluid of humans and rats (1-3).
- Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
- Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
- Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Product Datasheets
Citations for Mouse NGFR/TNFRSF16 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Closing the Gap between the Auditory Nerve and Cochlear Implant Electrodes: Which Neurotrophin Cocktail Performs Best for Axonal Outgrowth and Is Electrical Stimulation Beneficial?
Authors: D Schmidbaue, S Fink, F Rousset, H Löwenheim, P Senn, R Glueckert
International Journal of Molecular Sciences, 2023-01-19;24(3):.
Species: Mouse
Sample Types: Organoid
Applications: IHC -
Tiered Sympathetic Control of Cardiac Function Revealed by Viral Tracing and Single Cell Transcriptome Profiling
Authors: S Sharma, R Littman, J Tompkins, D Arneson, J Contreras, AH Dajani, K Ang, A Tsanhani, X Sun, PY Jay, H Herzog, X Yang, OA Ajijola
bioRxiv : the preprint server for biology, 2023-01-19;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Etiological Roles of p75NTR in a Mouse Model of Wet Age-Related Macular Degeneration
Authors: PV Subirada, A Tovo, MV Vaglienti, JD Luna Pinto, HU Saragovi, MC Sánchez, A Anastasía, PF Barcelona
Cells, 2023-01-12;12(2):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sterol O-Acyltransferase Inhibition Ameliorates High-Fat Diet-Induced Renal Fibrosis and Tertiary Lymphoid Tissue Maturation after Ischemic Reperfusion Injury
Authors: Y Ariyasu, Y Sato, Y Isobe, K Taniguchi, M Yanagita, M Arita
International Journal of Molecular Sciences, 2022-12-07;23(24):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A Novel Neuroprotective Mechanism for Lithium That Prevents Association of the p75NTR-Sortilin Receptor Complex and Attenuates proNGF-Induced Neuronal Death In Vitro and In Vivo
Authors: SG Greenwood, L Montroull, M Volosin, HE Scharfman, KK Teng, M Light, R Torkin, F Maxfield, BL Hempstead, WJ Friedman
eNeuro, 2018-01-17;5(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
Sci Rep, 2017-07-11;7(1):5127.
Species: Human
Sample Types: Urine
Applications: ELISA Development (Detection) -
Superior Cervical Ganglia Neurons Induce Foxp3+ Regulatory T Cells via Calcitonin Gene-Related Peptide
Authors: K Szklany, E Ruiter, F Mian, W Kunze, J Bienenstoc, P Forsythe, K Karimi
PLoS ONE, 2016-03-29;11(3):e0152443.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Semaphorin 3E secreted by damaged hepatocytes regulates the sinusoidal regeneration and liver fibrosis during liver regeneration.
Authors: Yagai T, Miyajima A, Tanaka M
Am J Pathol, 2014-06-12;184(8):2250-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
J Biol Chem, 2013-03-07;288(16):11144-54.
Species: Human, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Mouse natural killer (NK) cells express the nerve growth factor receptor TrkA, which is dynamically regulated.
Authors: Ralainirina N, Brons NH, Ammerlaan W, Hoffmann C, Hentges F, Zimmer J
PLoS ONE, 2010-12-01;5(12):e15053.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain.
Authors: Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU
Mol. Ther., 2010-07-27;18(12):2164-72.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-Fr
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2 microgram per mL Primary antibody dilution.
Primary antibody dilution: 5 microgram per mL
Postnatal day 7 mouse cerebellar tissue.
PN18 mouse
Closed Head Injury
Dilution 1:500
Frozen section. 20um thickness. Antibody dilution 1/500.
Cryostat Frozen section. 20um ticknes. Antibody dilution 1/500 ON 4C.
