Home / Nestin / Mouse/Rat Nestin Antibody

Mouse/Rat Nestin Antibody

Catalog #: MAB2736
19 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2736
MAB2736-SP
Nestin in Rat Cortical Stem Cells.
2 Images
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Nestin Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Nestin in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 307501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat Nestin
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti-Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Nestin in C2C12 Mouse Cell Line. Nestin was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse/Rat Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Clozapine Increases Nestin Concentration in the Adult Male Rat Hippocampus: A Preliminary Study
    Authors: H Kayir, BW Jenkins, B Alural, JY Khokhar
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13
    Authors: C Maranga, C Pereira, AC Raposo, A Vieira, S Duarte, EP Bekman, I Milagre, ST da Rocha
    Stem Cell Research, 2022-03-21;61(0):102757.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Specific Increase in Joint Neutrophil Extracellular Traps and Its Relation to Interleukin 6 in Autoimmune Arthritis
    Authors: A Ohyama, A Osada, H Kawaguchi, I Kurata, T Nishiyama, T Iwai, A Ishigami, Y Kondo, H Tsuboi, T Sumida, I Matsumoto
    International Journal of Molecular Sciences, 2021-07-16;22(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adult kidney stem/progenitor cells contribute to regeneration through the secretion of trophic factors
    Authors: K Tsuji, S Kitamura, Y Sang, K Fukushima, J Wada
    Stem Cell Res, 2020-05-31;46(0):101865.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Localization of phosphotyrosine adaptor protein ShcD/SHC4 in the adult rat central nervous system
    Authors: HN Robeson, HR Lau, LA New, J Lalonde, JN Armstrong, N Jones
    BMC Neurosci, 2019-12-10;20(1):57.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking neural stem cell-derived extracellular vesicles
    Authors: A Yoshimura, N Adachi, H Matsuno, M Kawamata, Y Yoshioka, H Kikuchi, H Odaka, T Numakawa, H Kunugi, T Ochiya, Y Tamai
    Dis Model Mech, 2018-01-30;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  7. Curcumin Increase the Expression of Neural Stem/Progenitor Cells and Improves Functional Recovery after Spinal Cord Injury
    Authors: WS Bang, KT Kim, YJ Seo, DC Cho, JK Sung, CH Kim
    J Korean Neurosurg Soc, 2017-12-29;61(1):10-18.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  8. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
    Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
    Acta Neuropathol Commun, 2017-11-30;5(1):92.
    Species: Primate
    Sample Types: Whole Cells
    Applications: ICC
  9. An Immortalized Human Dorsal Root Ganglia Cell Line Provides a Novel Context to Study Herpes Simplex Virus Type-1 Latency and Reactivation
    Authors: NM Thellman, C Botting, Z Madaj, SJ Triezenber
    J. Virol., 2017-05-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  11. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
    Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
    Stem Cell Res, 2017-03-07;20(0):76-79.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells.
    Authors: Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S
    PLoS ONE, 2015-08-12;10(8):e0134715.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation.
    Authors: Lyu J, Yu X, He L, Cheng T, Zhou J, Cheng C, Chen Z, Cheng G, Qiu Z, Zhou W
    Mol Brain, 2015-04-18;8(0):26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  14. Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
    Authors: Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S
    Stem Cells, 2014-06-01;32(6):1493-502.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Nestin regulates proliferation, migration, invasion and stemness of lung adenocarcinoma.
    Authors: Narita K, Matsuda Y, Seike M, Naito Z, Gemma A, Ishiwata T
    Int J Oncol, 2014-01-24;44(4):1118-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice.
    Authors: Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T
    Am J Pathol, 2014-01-09;184(3):674-85.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P
  17. The pleiotrophin-ALK axis is required for tumorigenicity of glioblastoma stem cells.
    Authors: Koyama-Nasu, R, Haruta, R, Nasu-Nishimura, Y, Taniue, K, Katou, Y, Shirahige, K, Todo, T, Ino, Y, Mukasa, A, Saito, N, Matsui, M, Takahashi, R, Hoshino-Okubo, A, Sugano, H, Manabe, E, Funato, K, Akiyama, T
    Oncogene, 2013-05-20;33(17):2236-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011-01-13;6(1):e16211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. VEGF-mediated angiogenesis stimulates neural stem cell proliferation and differentiation in the premature brain.
    Authors: Sun J, Sha B, Zhou W, Yang Y
    Biochem. Biophys. Res. Commun., 2010-02-24;394(1):146-52.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse/Rat Nestin Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse/Rat Nestin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse/Rat Nestin Antibody
By Anonymous on 10/26/2021
Application: ICC/IF Sample Tested: iPS cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736
Mouse/Rat Nestin Antibody
By Anonymous on 09/04/2021
Application: ICC/IF Sample Tested: epithelial cells Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736
Mouse/Rat Nestin Antibody
By Anonymous on 08/19/2019
Application: ICC/IF Sample Tested: Neural stem cells derived from h9 Species: Human

Neural stem cells were fixed in 4%PFA for 15 minutes. The nestin antibody was used at 10ug/ml at 4 degree overnight. Secondary antibody was incubated at room temperature for 1 hour.

Immunocytochemistry/Immunofluorescence Mouse/Rat Nestin Antibody MAB2736