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Mouse/Rat Nestin PE-conjugated Antibody

Catalog #: IC2736P
5 Citations Datasheet / COA / SDS
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IC2736P
Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Nestin PE-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Nestin in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 307501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Nestin antibody in Mouse Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry. Mouse cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE-conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Nestin antibody in Rat Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of Nestin in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE-conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Nestin

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14). Over amino acids (aa) 544-820, rat Nestin shares 75% and 38% sequence identity with mouse and human Nestin, respectively.

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

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Citations for Mouse/Rat Nestin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
    Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
    Int J Nanomedicine, 2017-02-17;12(0):1369-1384.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile
    J Immunol, 2016-04-29;196(11):4760-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
    Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M
    PLoS ONE, 2014-08-18;9(8):e105219.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
    Authors: Mutnal MB, Cheeran MC, Hu S
    PLoS ONE, 2011-01-13;6(1):e16211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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