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Mouse SCD-1 Antibody

Catalog #: MAB4404
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4404
MAB4404-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SCD-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SCD-1 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 428208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse SCD-1
Met1-Ser355
Accession # P13516
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse SCD‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCD-1

Stearoyl-CoA Desaturase 1 (SCD-1) is a four-transmembrane endoplasmic reticulum protein that is a rate-limiting enzyme in the formation of triglycerides, cholesteryl esters and phospholipids from saturated fatty acids. Its expression in adipose and liver is enhanced by insulin and certain dietary sugars and fats, and down‑regulated by TNF-alpha. SCD-1 is one of four iron-containing murine stearoyl-CoA desaturases that share 79‑88% aa identity. The 355 aa, predicted 41 kDa mouse SCD-1 shares 92% and 84% aa identity with rat and human SCD-1, respectively.

Long Name
Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase
Entrez Gene IDs
6319 (Human); 20249 (Mouse)
Alternate Names
FADS5; MSTP008; SCD1; SCD-1; SCDOS; Stearoyl-CoA desaturase

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Citation for Mouse SCD-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interfering with stem cell-specific gatekeeper functions controls tumour initiation and malignant progression of skin tumours.
    Authors: Petersson, Monika, Reuter, Karen, Brylka, Heike, Kraus, Andreas, Schettina, Peter, Niemann, Catherin
    Nat Commun, 2015-01-22;6(0):5874.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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