Mouse ST2/IL-33R Biotinylated Antibody

Catalog #: BAF1004
1 Citation Datasheet / COA / SDS

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BAF1004

All ST2/IL-33R Antibodies

Product Details

Citations (1)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ST2/IL-33R Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ST2/IL-33 R in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) IL-1 R4, recombinant mouse (rm) IL-1 R1, rmIL-2 R2, rhIL-1 R4, rmIL-1 R6, rmIL-1 R8, and rhIL-1 R9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Ser342
Accession # P14719

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ST2/IL-33 R Fc Chimera (Catalog # 1004-MR)

Mouse ST2/IL-33 R Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse ST2/IL-33R Antibody (Catalog # MAB10041)

Standard: Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF (Catalog # 1004-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).

References

Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
Schmitz, J. et al. (2005) Immunity 23:479.
Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
Weinberg, E.O. et al. (2002) Circulation 106:2961.
Sanada S. et al. (2007) J. Clin. Invest. 117:1538
Palmer, G. et al. (2008) Cytokine 42:358.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Alternate Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse ST2/IL-33R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells.
Authors: Drube S, Heink S, Walter S, Lohn T, Grusser M, Gerbaulet A, Berod L, Schons J, Dudeck A, Freitag J, Grotha S, Reich D, Rudeschko O, Norgauer J, Hartmann K, Roers A, Kamradt T
Blood, 2010-03-03;115(19):3899-906.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot