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Reagent Diluent Concentrate 2

Catalog #: DY995
36 Citations 24 Reviews Datasheet / COA / SDS
10X, 5 x 21 mL
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Reagent Diluent Concentrate 2
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Citations (36)
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Reviews (24)
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Reagent Diluent Concentrate 2 Summary

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

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Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
    Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
    Scientific Reports, 2022-11-30;12(1):20622.  2022-11-30
  2. Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
    Authors: H Kim, B Jang, YJ Kim, JC Choi
    International Journal of Molecular Sciences, 2022-09-11;23(18):.  2022-09-11
  3. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
    Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
    Nature, 2021-11-03;0(0):.  2021-11-03
  4. Topological isolation of developmental regulators in mammalian genomes
    Authors: HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor
    Nature Communications, 2021-08-12;12(1):4897.  2021-08-12
  5. The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
    Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
    Embo Molecular Medicine, 2021-03-10;0(0):e13162.  2021-03-10
  6. Detection of Plasmodium falciparum malaria in 1�h using a simplified enzyme-linked immunosorbent assay
    Authors: E de la Sern, K Arias-Alpí, LN Borgheti-C, A Sanchez-Ca, E Sulleiro, F Zarzuela, P Bosch-Nico, F Salvador, I Molina, M Ramírez, X Fernàndez-, A Sánchez-Mo, E Baldrich
    Analytica chimica acta, 2021-01-30;1152(0):338254.  2021-01-30
  7. Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
    Authors: L Kang, S Smith, C Wang
    Journal of colloid and interface science, 2020-12-24;588(0):101-109.  2020-12-24
  8. Differentially expressed circular RNAs in a murine asthma model
    Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
    Molecular Medicine Reports, 2020-10-20;22(6):5412-5422.  2020-10-20
  9. bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
    Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
    Sci Rep, 2020-09-29;10(1):15968.  2020-09-29
  10. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
    Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
    J Immunol Res, 2020-09-11;2020(0):9690832.  2020-09-11
  11. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.  2020-02-10
  12. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019-08-01;14(0):6117-6131.  2019-08-01
  13. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019-07-15;8(7):.  2019-07-15
  14. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019-04-11;2019(0):1845128.  2019-04-11
  15. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018-09-25;0(0):9636897187922.  2018-09-25
  16. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018-07-09;8(1):10328.  2018-07-09
  17. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018-02-01;2018(0):8286067.  2018-02-01
  18. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018-01-08;0(0):.  2018-01-08
  19. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017-05-18;2(10):.  2017-05-18
  20. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017-04-21;13(6):4995-5001.  2017-04-21
  21. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017-03-13;13(5):3269-3276.  2017-03-13
  22. Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
    Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
    Am J Physiol Regul Integr Comp Physiol, 2015-06-24;309(5):R467-74.  2015-06-24
  23. Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
    Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
    Mol Neurodegener, 2014-06-06;9(0):22.  2014-06-06
  24. Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
    Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
    Nat Commun, 2014-04-30;5(0):3779.  2014-04-30
  25. Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
    Authors: Litzlbauer P, Weber K, Mueller R
    Cytokine, 2014-01-14;66(1):54-9.  2014-01-14
  26. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012-06-20;7(6):e39036.  2012-06-20
  27. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011-10-21;187(11):5745-55.  2011-10-21
  28. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011-04-19;19(10):1793-801.  2011-04-19
  29. Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
    Authors: Derycke MS, Pambuccian SE, Gilks CB
    Am. J. Clin. Pathol., 2010-11-01;134(5):835-45.  2010-11-01
  30. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010-07-01;85(7):487-93.  2010-07-01
  31. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.  2010-02-16
  32. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2009-12-10;33(3):664-9.  2009-12-10
  33. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008-02-25;38(4):594-601.  2008-02-25
  34. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008-02-08;4(2):e41.  2008-02-08
  35. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007-08-01;67(15):7458-66.  2007-08-01
  36. Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
    Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
    J. Neurochem., 2006-02-08;96(6):1560-71.  2006-02-08

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Reviews for Reagent Diluent Concentrate 2

Average Rating: 5 (Based on 24 Reviews)

5 Star
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Reagent Diluent Concentrate 2
By Anonymous on 05/23/2022

Great for use in ELISAs. Consistent product and convenient to use.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 09/23/2021
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/31/2021

Effective blocking reagent for FACs binding assays. The multiple vials make them easy for one time use on days of assays without having to worry about contaminating a large stock.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Hon Wai Koon on 12/01/2020
Reagent Diluent Concentrate 2
By Anonymous on 11/22/2020
Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/17/2020
Reagent Diluent Concentrate 2
By Anonymous on 01/10/2020
Reagent Diluent Concentrate 2
By Tahmineh Safaie on 12/23/2019

I have been using the this for several ELISA assays and has been working with no problem

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 09/23/2019

excellent blocker, we used 7 kits so far. Works well for serum ELISA's

Reagent Diluent Concentrate 2
By Anonymous on 03/28/2019

I use this as my blocking buffer and antibody diluent buffer in all my ELISAs. I like that it comes as a concentrate

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Marry Tahani on 11/20/2018

Gives really good linearity.

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 08/17/2018
Reagent Diluent Concentrate 2
By Anonymous on 07/17/2018

Great blocker

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 06/15/2018
Reagent Diluent Concentrate 2
By Anonymous on 04/30/2018
Reagent Diluent Concentrate 2
By Anonymous on 04/11/2018
Reagent Diluent Concentrate 2
By Leslie Priddy on 04/03/2018
Reagent Diluent Concentrate 2
By Anonymous on 02/06/2018
Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018

I use RD to make standard curves

Reagent Diluent Concentrate 2 DY995
Reagent Diluent Concentrate 2
By Anonymous on 01/19/2018
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/19/2017

Used the reagent diluent for assay and it works perfect has very little to no background

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL) DY995
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Shubhanchi Nigam on 07/14/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL) DY995
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 07/06/2017
Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)
By Anonymous on 03/14/2017

Used for Duoset ELISA