Recombinant Human Follistatin (aa 30-344) Protein

Carrier Free

Catalog # Availability Size / Price Qty
4889-FN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4889-FN-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
FAQs
Reviews (1)

Recombinant Human Follistatin (aa 30-344) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.01‑0.05 µg/mL in the presence of 7.5 ng/mL of rhActivin A.
Source
Chinese Hamster Ovary cell line, CHO-derived human Follistatin protein
Gly30-Trp344
Accession #
N-terminal Sequence
Analysis
Gly30
Predicted Molecular Mass
34.7 kDa
SDS-PAGE
40-50 kDa, reducing conditions

Product Datasheets

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4889-FN (with carrier)

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4889-FN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4889-FN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4889-FN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin

Follistatin (FST) is a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human Follistatin cDNA encodes a 344 amino acid (aa) protein with a 29 aa signal sequence, an N-terminal atypical TGF binding domain, three Follistatin domains that contain EGF-like and kazal-like motifs, and a highly acidic C-terminal tail. The first Follistatin domain (FS1) contains a heparin binding site, while FS1 and FS2 are most critical for activin binding and neutralization (3, 4). In addition to activin, Follistatin regulates bioavailability of many non-TGF-beta members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). It also regulates hematopoietic stem cell adhesion to fibronectin via FS2, and binds angiogenin via FS2 and FS3 (6, 7). Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3 (3, 5, 6). Of three Follistatin isoforms, the full-length mature Follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative isoforms (5, 8, 9). The acidic tail is missing in the splice variant FST288 which shows the highest affinity for activins, while a partial tail exists in the proteolytically produced FST303, which shows intermediate activin affinity (5, 8, 9). FST315 shares 98% aa identity with mouse, rat, equine and ovine FST, 99% with porcine and 97% with bovine FST. Genetic deletion of Follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility (10).

References
  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Citations for Recombinant Human Follistatin (aa 30-344) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Neuroadaptations in the dorsal hippocampus underlie cocaine seeking during prolonged abstinence
    Authors: CT Werner, S Mitra, BD Auerbach, ZJ Wang, JA Martin, AF Stewart, PH Gobira, M Iida, C An, MM Cobb, A Caccamise, RJ Salvi, RL Neve, AM Gancarz, DM Dietz
    Proc Natl Acad Sci U S A, 2020-10-05;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Involvement of activin signal pathway in cyclic apoptosis of the oviductal isthmic epithelium in cows
    Authors: Y Yamamoto, S Ito, K Okuda, K Kimura
    Theriogenology, 2020-05-18;153(0):143-150.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Inhibition of bone morphogenic protein 4 restores endothelial function in db/db diabetic mice.
    Authors: Zhang Y, Liu J, Tian X, Wong W, Chen Y, Wang L, Luo J, Cheang W, Lau C, Kwan K, Wang N, Yao X, Huang Y
    Arterioscler Thromb Vasc Biol, 2013-11-07;34(1):152-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Human Follistatin (aa 30-344) Protein
By Anonymous on 07/08/2020
Application: Binding assay/Protein-protein interaction