Recombinant Human SOST/Sclerostin Protein Summary
Product Specifications
Gln24-Tyr213, with an N-terminal 7-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1406-ST
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1406-ST/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SOST/Sclerostin
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homesotasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1-3).
Citations for Recombinant Human SOST/Sclerostin Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice
Authors: N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou
Frontiers in Physiology, 2023-01-04;13(0):1061715.
Species: N/A
Sample Types: Buffer
Applications: Western Blot -
A Versatile Macromer-Based Glycosaminoglycan (sHA3) Decorated Biomaterial for Pro-Osteogenic Scavenging of Wnt Antagonists
Authors: M Gronbach, F Mitrach, S Möller, S Rother, S Friebe, SG Mayr, M Schnabelra, V Hintze, MC Hacker, M Schulz-Sie
Pharmaceutics, 2020-10-29;12(11):.
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance -
Vitamin D-regulated osteocytic sclerostin and BMP2 modulate uremic extraskeletal calcification
Authors: L Nguyen-Yam, KI Tanaka, R St-Arnaud, D Goltzman
JCI Insight, 2019-07-11;4(13):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement
Authors: F Ohori, H Kitaura, A Marahleh, A Kishikawa, S Ogawa, J Qi, WR Shen, T Noguchi, Y Nara, I Mizoguchi
J Immunol Res, 2019-06-24;2019(0):9716758.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Osteocyte-Intrinsic TGF-? Signaling Regulates Bone Quality through Perilacunar/Canalicular Remodeling
Authors: NS Dole, CM Mazur, C Acevedo, JP Lopez, DA Monteiro, TW Fowler, B Gludovatz, F Walsh, JN Regan, S Messina, DS Evans, TF Lang, B Zhang, RO Ritchie, KS Mohammad, T Alliston
Cell Rep, 2017-11-28;21(9):2585-2596.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
Authors: R Shu, D Bai, T Sheu, Y He, X Yang, C Xue, Y He, M Zhao, X Han
PLoS ONE, 2017-01-12;12(1):e0167312.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sclerostin regulates release of bone mineral by osteocytes by induction of carbonic anhydrase 2.
Authors: Kogawa M, Wijenayaka A, Ormsby R, Thomas G, Anderson P, Bonewald L, Findlay D, Atkins G
J Bone Miner Res, 2013-12-01;28(12):2436-48.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altered skeletal expression of sclerostin and its link to radiographic progression in ankylosing spondylitis.
Authors: Appel H, Ruiz-Heiland G, Listing J, Zwerina J, Herrmann M, Mueller R, Haibel H, Baraliakos X, Hempfing A, Rudwaleit M, Sieper J, Schett G
Arthritis Rheum., 2009-11-01;60(11):3257-62.
Applications: ELISA Developmet -
Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro.
Authors: Jager A, Gotz W, Lossdorfer S, Rath-Deschner B
J. Periodont. Res., 2009-09-23;45(2):246-54.
Species: Human
Sample Types:
Applications: Western Blot
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