Recombinant Mouse GASP-1/WFIKKN2 Protein Summary
Product Specifications
Met1-Gln571, With a C-Terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9956-GS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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9956-GS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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Scientific Data
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Recombinant Mouse GASP-1/WFIKKN2 (Catalog # 9956-GS) dose dependently inhibits Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-G8) activity in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 0.02-0.12 µg/mL.
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2 μg/lane of Recombinant Mouse GASP‑1/WFIKKN2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 63-77 kDa and 55-66 kDa, respectively.
Reconstitution Calculator
Background: GASP-1/WFIKKN2
Growth and differentiation factor-associated serum protein 1 (GASP-1), also known as WAP, Follistatin/Kazal, Immunoglobulin, Kunitz and Netrin domain containing protein 2 (WFIKKN2), appears to be a multifunctional molecule comprised of several conserved domains. GASP-1, along with the highly related GASP-2 (WFIKKN1), contains a WAP domain, a Follistatin/Kazal domain, an Immunoglobulin domain, two Kunitz‐type protease inhibitor domains and a netrin domain (1). GASP-1 was first isolated in a screen to identify proteins in mice that copurify with Myostatin (GDF8) (1). Mature mouse GASP-1 is 542 amino acids (aa) and shares 94% and 98% aa identity with mature human and rat GASP-1, respectively.
WAP, Follistatin, Kazal and Netrin domains are all implicated in protease inhibition, and GASP-1 and 2 may be protease inhibitor proteins involved in muscle development (1). In humans, GASP-1 and 2 show distinct expression patterns, with GASP-1 expression during development highest in the brain, skeletal muscle, thymus and kidney (2). In mice, GASP-1 expression can be detected in the neural tube and limb buds of developing embryos, while in adult mice it is found in numerous tissue types (3, 4). Both GASP-1 and 2 appear to bind several members of the TGF beta family with high affinity, including Activin, BMP2, BMP4, TGF-beta 1, GDF-8 and GDF-11 (1, 5, 6). Interestingly, GASP-1 has been shown to specifically inhibit the activity of GDF-8 and GDF-11 but not the other TGF-beta family proteins (1, 4, 6). GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). Over expression of GASP-1 in mice results in animals that are hyper muscled, consistent with reduction in GDF-8 signaling (7).
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
- Monestier, O. et al. (2012) PLoS One 7(8):e43710.
- Lee, Y and Lee S. (2013) PNAS 110(39).
- Kondas, K. et al. (2008) JBC 283:23677.
- Szlama, G. et al. (2010) FEBS J 277:5040.
- Monestier, O. et al. (2012) BMC Genomics 13(1):541.
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