Recombinant Mouse PDGF R alpha His-tag Protein, CF

Catalog #: 10065-PR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10065-PR-050

Recombinant Mouse PDGF R alpha His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PDGF R alpha His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse PDGF R alpha is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Rat PDGF-AA (Catalog # 1055-AA) that produces 50% of the optimal binding response is 0.75-7.5 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse PDGF R alpha protein
Leu25-Glu524, with a C-terminal 6-His tag

Accession #

P26618.3

N-terminal Sequence
Analysis

Leu25

Predicted Molecular Mass

57 kDa

SDS-PAGE

86-97 kDa, reducing conditions

Product Datasheets

10065-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10065-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse PDGF Ra (Catalog # 10065-PR) is coated at 1 µg/mL, 100 µL/well, Recombinant Rat PDGF-AA (Catalog # 1055-AA) binds with an ED₅₀ of 0.75-7.5 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse PDGF R alpha was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-97 kDa.

Reconstitution Calculator

Background: PDGF R alpha

PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1-3). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of mouse PDGF R alpha (amino acids 25-525) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593‑954). Within the ECD, mouse PDGF R alpha shares 85%, 93%, 84%, 84%, and 81% amino acid sequence identity with human, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1-3). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 4). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (5- 7).

References

Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Do, M.S. et al. (1992) Oncogene 7:1567.
Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103.
Martinho, O. (2009) Br. J. Cancer 101:973.
Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303.
Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.

Long Name

Platelet-derived Growth Factor Receptor alpha

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Alternate Names

alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA