Simple Plex Human NGFR/TNFRSF16 Cartridge

Catalog #: SPCKB-PS-000297 Datasheet / COA / SDS

Simple Plex Human NGFR/TNFRSF16 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

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SPCKB-PS-000297
SPCKC-PS-000297
SPCKA-PS-000297

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898345: Simple Plex Control for Human NGFR/TNFRSF16

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human NGFR/TNFRSF16 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Urine (25 uL)

Sensitivity

12.7 pg/mL

Assay Range

28.6 - 108,900 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Natural and recombinant human NGF R.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human NGF R in serum, plasma (EDTA/Heparin), and urine

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	9	8
Mean (pg/mL)	613	32027	597	35057
Standard Deviation	28.5	2331	39.1	2601
CV%	4.7	7.3	6.5	7.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	112	101-122
Heparin Plasma (n=4)	106	88-119
Serum (n=4)	108	98-115

Linearity

Samples containing and/or spiked with high concentrations of human NGF R were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human NGF R Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NGFR/TNFRSF16

The low affinity nerve growth factor receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family1 and has been designated TNFRSF16. The NGF receptor is also known as p75 NTR (neurotrophin receptor) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. NGF R also binds several myelin derived proteins (Nogo, myelin associated glycoprotein, and myelin oligodendrocyte glycoprotein) as a co-receptor with Nogo receptor.

The NGF receptor is a 75 kDa protein that is expressed in neuronal axons, Schwanns cells and perineural cells of peripheral nerves. Neural crest stem cells have been isolated based on their surface expression of NGF R. In addition, neuroepithelial-derived NGF receptor positive cells have also been demonstrated to be able to differentiate into neurons, smooth muscle and Schwann cells in culture. The expression of the NGF receptor has also been used as a marker to identify mesenchymal precursors as well as hepatic stellate cells.

Long Name:

Nerve Growth Factor Receptor

Entrez Gene IDs:

4804 (Human); 18053 (Mouse); 117089 (Rat)

Alternate Names:

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16