Flow cytometry is essential for the study of innate lymphoid cells (ILCs). It allows multiparameter analysis of cell populations based on the expression of specific cell surface and/or intracellular molecules. Consensus gating strategies were established in collaboration with researchers in the ILCs field.1,2 For ILC single cell suspension preparation, R&D systems scientists used previously established protocols.3,4
Group 1 ILCs |
|
| Natural killer cells | |
| Mouse | CD3-CD49b+CD161+NKp46+CD27high/low |
| Human | CD3- CD57+CD56dim/brightCD16dim/- |
| ILC1 | |
| Mouse/Human | CD34- CD127+NKp44-CD117- |
| IEL | |
| Mouse | CD45+CD3 epsilon-CD19-CD161+NKp46+CD160+ |
| Human | CD45+CD3 epsilon-CD19-NKp44+NKp46+CD103+ |
Group 2 ILCs |
|
| ILC2 | |
| Mouse/Human | CD45+CD3 epsilon-CD4-CD8-CD19-CD11b-CD161-CD90.2+T1/ST2+GATA3high |
Group 3 ILCs |
|
| LTi cells | |
| Mouse/Human | CD4+/-CD127+ Integrin alpha 4 beta 7+ROR gamma t+NKp46-CCR6+ |
| NCR-ILC3 | |
| Mouse | CD45+CD3-CD19-ROR gamma t+NKp46-CD161 T-bet+/- |
| Human | CD45+CD3-CD19-CD127+NKp44+CCR6-T-bet+/- |
| NCR+ ILC3 | |
| Mouse | CD45+CD3-CD19-ROR gamma t+NKp46+CD161low/- T-bethigh |
| Human | CD45+CD3-CD19-ROR gamma t+CD127+NKp44+CCR6+T-bethigh |
More ILC Resources:
R&D Systems Innate Lymphoid Cells Differentiation Pathway
R&D Systems optimized protocols for Flow Cytometry samples preparation
References:
- Fuchs, A. et al (2013) Immunity. 38:769
- Molofsky, A.B. et al (2013) J. Exp. Med. 210:535
- Morita, R. et al (2011) Immunity. 34: 108
- Hepworth, M.R. et al (2013) Nature. 498 :113