LI-COR Data Examples for the Human Oncology Array Kit

Data Examples

Profiling Proteins in Cell Culture Supernates

Figure 1. The Human XL Oncology Array detects multiple analytes in cell culture supernates. A549 human lung carcinoma cells were either cultured in normoxia or 1% O2 for 3 days. For each array shown, 500 µL of cell supernate was used. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

 

Profiling Proteins in Cell Culture Lysates

 

Figure 2. The Human XL Oncology Array detects multiple analytes in cell culture lysates. Lysates from SW480 human colorectal adenocarcinoma and SW620 human colorectal adenocarcinoma metastatic site cells. 200 µg of cell lysate was run on each array. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

 

Profiling Proteins In Tissue Lysate, SW620 Supernates & Body Fluids

 

1 Angiopoietin-1
2 MSP/MST1
3 E-Selectin
4 VCAM-1
5 IL-18 Bpa
6 Leptin
7 Mesothelin
8 MMP-3
9 Carbonic Anhydrase IX
10 EpCAM/TROP-1
11 HGF R
12 Tenascin C

Figure 3. The Human XL Oncology Array detects multiple analytes in serum, plasma and SW620 supernate samples.

The sample type and quantity used per array is listed. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Odyssey is registered trademarks of LI-COR, Inc.