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Human uPAR Biotinylated Antibody

Catalog #: BAF807
10 Citations Datasheet / COA / SDS
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BAF807
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Citations (10)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human uPAR Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human uPAR in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse uPAR and recombinant human uPA-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human uPAR (R&D Systems, Catalog # 807-UK)
Leu23-Arg303
Accession # Q03405
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human uPAR (Catalog # 807-UK)

Human uPAR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human uPAR Antibody (Catalog # MAB807)

Standard: Recombinant Human uPAR Protein (Catalog # 807-UK)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: uPAR

The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.

References
  1. Dear, A.E. and R.L. Medcalf (1998) Eur. J. Biochemistry 252:185.
Long Name
Urokinase-type Plasminogen Activator Receptor
Entrez Gene IDs
5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)
Alternate Names
CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3

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Citations for Human uPAR Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Mimicking Intermolecular Interactions of Tight Protein-Protein Complexes for Small-Molecule Antagonists
    Authors: David Xu, Khuchtumur Bum-Erdene, Yubing Si, Donghui Zhou, Mona K. Ghozayel, Samy O. Meroueh
    ChemMedChem
  2. A Computational Investigation of Small-Molecule Engagement of Hot Spots at Protein–Protein Interaction Interfaces
    Authors: David Xu, Khuchtumur Bum-Erdene, Yubing Si, Donghui Zhou, Degang Liu, Mona Ghozayel et al.
    Journal of Chemical Information and Modeling
  3. Small Molecules Engage Hot Spots through Cooperative Binding To Inhibit a Tight Protein–Protein Interaction
    Authors: Degang Liu, David Xu, Min Liu, William Eric Knabe, Cai Yuan, Donghui Zhou et al.
    Biochemistry
  4. A New Class of Orthosteric uPAR·uPA Small-Molecule Antagonists Are Allosteric Inhibitors of the uPAR·Vitronectin Interaction
    Authors: Degang Liu, Donghui Zhou, Bo Wang, William Eric Knabe, Samy O. Meroueh
    ACS Chemical Biology
  5. Probing Binding and Cellular Activity of Pyrrolidinone and Piperidinone Small Molecules Targeting the Urokinase Receptor
    Authors: Timmy Mani, Degang Liu, Donghui Zhou, Liwei Li, William Eric Knabe, Fang Wang et al.
    ChemMedChem
  6. Genome-wide protein QTL mapping identifies human plasma kallikrein as a post-translational regulator of serum uPAR levels.
    Authors: Portelli M, Siedlinski M, Stewart C, Postma D, Nieuwenhuis M, Vonk J, Nurnberg P, Altmuller J, Moffatt M, Wardlaw A, Parker S, Connolly M, Koppelman G, Sayers I
    FASEB J, 2013-11-18;28(2):923-34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Small-molecule inhibition of the uPAR·uPA interaction: Synthesis, biochemical, cellular, in vivo pharmacokinetics and efficacy studies in breast cancer metastasis
    Authors: Timmy Mani, Fang Wang, William Eric Knabe, Anthony L. Sinn, May Khanna, Inha Jo et al.
    Bioorganic & Medicinal Chemistry
  8. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  9. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  10. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
    Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
    Am. J. Respir. Cell Mol. Biol., 2006-05-25;35(4):503-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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