Mouse/Rat PDGF-BB DuoSet ELISA

Name: Mouse/Rat PDGF-BB DuoSet ELISA
Brand: R&D Systems
SKU: DY8464-05
Rating: 5 (1 reviews)

Catalog #: DY8464-05
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY8464-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

Mouse / Rat PDGF-BB ELISA Standard Curve

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All PDGF-BB ELISAs

Product Details

Citations (4)

FAQs

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Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse/Rat PDGF-BB DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

62.5 - 4,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Mouse / Rat PDGF-BB ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:

Platelet-derived Growth Factor BB

Entrez Gene IDs:

5155 (Human); 24628 (Rat)

Alternate Names:

c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV; PDGFBB; PDGF-BB

Citations for Mouse/Rat PDGF-BB DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfbeta Signaling in the Tumor Microenvironment
Authors: Y Zhang, E Manouchehr, M Herre, J Cedervall, Q Qiao, Z Miao, A Hamidi, L Hellman, M Kamali-Mog, AK Olsson
Cancers, 2022-04-12;14(8):.
Species: Mouse
Sample Types: Tissue Lysate
GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation
Authors: T Castro-Dop, A Fleming, TW Dennison, JR Ferdinand, K Harcourt, BJ Stewart, Z Cader, ZK Tuong, C Jing, LSC Lok, RJ Mathews, A Portet, A Kaser, S Clare, MR Clatworthy
Cell Rep, 2020-07-07;32(1):107857.
Species: Mouse
Sample Types: Cell Culture Supernates
Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
Authors: Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino
Sci Adv, 2020-06-12;6(24):eaba7602.
Species: Mouse
Sample Types: Extracellular Matrix
Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
Authors: Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino
Sci Adv, 2020;6(24):eaba7602.
Species: Mouse
Sample Types: Extracellular Matrix