Human GASP-2/WFIKKN Antibody

Catalog #: MAB21361 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB21361
MAB21361-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GASP-2/WFIKKN Antibody Summary

Species Reactivity

Human

Specificity

Detects human GASP-2/WFIKKN in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human GASP-1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 289827

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GASP-2/WFIKKN
Ala20-Asp548
Accession # Q96NZ8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human GASP-2/WFIKKN Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human GASP‑2/WFIKKN Biotinylated Antibody (Catalog # BAM21362)

Standard: Recombinant Human GASP-2/WFIKKN Protein (Catalog # 2136-GS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GASP-2/WFIKKN

Growth and differentiation factor-associated serum protein-2 (GASP-2) cDNA encodes a 548 amino acid protein that contains a 19 amino acid signal sequence and is comprised of many conserved domains: WAP, follistatin/Kazal, immunoglobulin, two tandem Kunitz, and netrin (1). Based on the order of these conserved domains, GASP-2 is also known as WFIKKN (1). Another related protein which contains the same domain structure is called WFIKKNRP (WFIKKN-related protein), or GASP-1 (2, 3). WAP, follistatin, Kunitz and netrin domains are all implicated in protease inhibition, and the GASP proteins may be multivalent protease inhibitors (1, 4). Tests at R&D Systems have measured the ability of GASP-2 to inhibit trypsin cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (R&D Systems Catalog # ES002). The IC₅₀ value was approximately 10 nM, as measured in a reaction mixture containing 1.0 nM trypsin, 10 µM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-2 is abundant in the lung, skeletal muscle and liver while GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney (3). In the adult, GASP-2 is expressed primarily in the pancreas, liver, and thymus while GASP-1 is in the ovary, testis, and brain (3). Further characterization shows that GASP-1 inhibits myostatin (GDF-8) and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (2). Although, this kind of activity has not been reported for GASP-2, tests at R&D Systems have determined that GASP-2 shows similar inhibitory activity towards myostatin as GASP-1. By amino acid sequence, human GASP-2 is 55% identical to human GASP-1.

References

Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3705.
Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
Trexler, M. et al. (2002) Biol. Chem. 383:223.
Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.

Long Name

Growth and Differentiation Factor-associated Serum Protein-2

Entrez Gene IDs

117166 (Human); 215001 (Mouse)

Alternate Names

C16orf12; Chromosome 16 Open Reading Frame 12; GASP2; GASP-2; Growth And Differentiation Factor-Associated Serum Protein 2; hGASP-2; RJD2; WAP Four-Disulfide Core Domain 20A; WAP, Follistatin/Kazal, Immunoglobulin, Kunitz And Netrin Domain Containing 1; WAP, FS, Ig, KU, And NTR-Containing Protein; WFDC20A; WFIKKN; WFIKKN1