Mouse DAN Antibody

Catalog #: MAB7552 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7552
MAB7552-SP

All DAN Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse DAN Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse DAN in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) DAN, rhCOCO, recombinant mouse (rm) BMP-4, rmGremlin, or recombinant chicken (rch) Caronte is observed. In Western blots, approximately 10% cross-reactivity with rhDAN, rhCOCO, and rchCaronte is observed and no cross reactivity with rmGremlin is observed.

Source

Monoclonal Rat IgG_2A Clone # 123224

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse DAN
Ala17-Asp178
Accession # Q61477

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse DAN (Catalog # 755-DA) under non-reducing conditions only

Mouse DAN Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse DAN Biotinylated Antibody (Catalog # BAF755)

Standard: Recombinant Mouse DAN Protein (Catalog # 755-DA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAN

DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is down‑regulated in src-transformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP-2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA. It is likely the various DAN family members and other TGF-beta BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGF-beta superfamily ligands.

References

Massage, J. and Y-G. Chen (2000) Genes & Development 14:627.
Perch, J.J.H. et al. (1999) Develop. Biol. 209:98.
Hsu, D.R. et al. (1998) Mol. Cell. 1:673.

Long Name

Differential Screening-selected Gene Aberrant in Neuroblastoma

Entrez Gene IDs

4681 (Human); 17965 (Mouse)

Alternate Names

DAN domain family member 1; DAN; DAND1; MGC8972; NBL1; neuroblastoma suppressor of tumorigenicity 1; neuroblastoma, suppression of tumorigenicity 1; Protein N03; suppression of tumorigenicity 1; Zinc finger protein DAN