Induction and Analysis of Epithelial to Mesenchymal Transition

Joy Aho, PhD, Manager of Stem Cells Research and Development Bio-Techne
Ernesto Resnik, PhD, Manager of Antibody Research and Development Bio-Techne

In this peer-reviewed video publication, featured in the Journal of Visualized Experiments (JoVE), Bio-Techne scientists provide data supporting new methods for reliably inducing epithelial to mesenchymal transition (EMT) in vitro. EMT is essential for proper morphogenesis during development. Misregulation of EMT is attributed to fibrosis and the progression of carcinomas metastasis. Creating reliable cell culture models for EMT induction and analysis is key for gaining insights into the field of wound healing and cancer metastasis. In this video, we demonstrate a straightforward method for the induction of EMT in a variety of cell types, methods for pre- and post-EMT induction analysis, including antibody staining, antibody-based array analysis, and migration/invasion assays.

This video includes:

• Overview of EMT
• Challenges in EMT induction and model systems
• Comparison of methods for EMT induction
• Techniques for EMT analysis
  • Antibody markers
  • Cell invasion assays

Download our Epithelial to Mesenchymal Transition graphical poster.
Check out more data on our StemXVivo® EMT Induction Media Supplement here.
View our full range of products for Epithelial to Mesenchymal Transition.