Proteome Profiler Mouse Cytokine Array Kit, Panel A
Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.
Key Benefits
- Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
CXCL13/BLC/BCA-1 | IL-5 | M-CSF |
C5a | IL-6 | CCL2/JE/MCP-1 |
G-CSF | IL-7 | CCL12/MCP-5 |
GM-CSF | IL-10 | CXCL9/MIG |
CCL1/I-309 | IL-12 p70 | CCL3/MIP-1 alpha |
CCL11/Eotaxin | IL-13 | CCL4/MIP-1 beta |
ICAM-1 | IL-16 | CXCL2/MIP-2 |
IFN-gamma | IL-17 | CCL5/RANTES |
IL-1 alpha/IL-1F1 | IL-23 | CXCL12/SDF-1 |
IL-1 beta/IL-1F2 | IL-27 | CCL17/TARC |
IL-1ra/IL-1F3 | CXCL10/IP-10 | TIMP-1 |
IL-2 | CXCL11/I-TAC | TNF-alpha |
IL-3 | CXCL1/KC | TREM-1 |
IL-4 |
Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A
Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems |
CXCL13/BLC/BCA-1 | MCX130 | DY470 |
C5a | DY2150 | |
G-CSF | MCS00 | DY414 |
GM-CSF | MGM00 | DY415 |
CCL1/I-309/TCA-3 | DY845 | |
CCL11/Eotaxin | MME00 | DY420 |
ICAM-1 | MIC100 | DY796 |
IFN-gamma | MIF00 | DY485 |
IL-1 alpha | MLA00 | DY400 |
IL-1 beta | MLB00B | DY401 |
IL-1ra | MRA00 | DY480 |
IL-2 | M2000 | DY402 |
IL-3 | M3000 | DY403 |
IL-4 | M4000B | DY404 |
IL-5 | M5000 | DY405 |
IL-6 | M6000B | DY406 |
IL-7 | M7000 | DY407 |
IL-10 | M1000b | DY417 |
IL-12 p70 | M1270 | DY419 |
IL-13 | M1300CB | DY413 |
IL-16 | ||
IL-17 | M1700 | DY421 |
IL-23 | M2300 | DY1887 |
IL-27 | M2728 | |
CXCL10/IP-10/CRG-2 | MCX100 | DY466 |
CXCL11/I-TAC | DY572 | |
CXCL1/KC | MKC00B | DY453 |
M-CSF | MMC00 | DY416 |
CCL2/MCP-1/JE | MJE00 | DY479 |
CCL12/MCP-5 | MCC120 | DY428 |
CXCL9/MIG | MCX900 | DY492 |
CCL3/MIP-1 alpha | MMA00 | DY450 |
CCL4/MIP-1 beta | MMB00 | DY451 |
CXCL2/MIP-2 | MM200 | DY452 |
CCL5/RANTES | MMR00 | DY478 |
CXCL12/SDF-1 | MCX120 | DY460 |
CCL17/TARC | DY529 | |
TIMP-1 | MTM100 | DY980 |
TNF-alpha | MTA00 | DY410 |
TREM-1 | MTRM10 | DY1187 |
Specifications
Product Datasheets
Scientific Data
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.
The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
International Journal of Molecular Sciences, 2022-02-07;23(3):. 2022-02-07
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The downregulation of type I IFN signaling in G-MDSCs under tumor conditions promotes their development towards an immunosuppressive phenotype
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Apiaceous vegetables protect against acrolein-induced pulmonary injuries through modulating hepatic detoxification and inflammation in C57BL/6 male mice
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The Journal of nutritional biochemistry, 2022-01-10;101(0):108939. 2022-01-10
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Elevated expression of the colony-stimulating factor 1 (CSF1) induces prostatic intraepithelial neoplasia dependent of epithelial-Gp130
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Journal of Neuroinflammation, 2022-01-04;19(1):2. 2022-01-04
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Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice
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A new method for obtaining bankable and expandable adult-like microglia in mice
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The Nutritional Supplement L-Alpha Glycerylphosphorylcholine Promotes Atherosclerosis
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Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Faslpr Mice
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Cells, 2021-11-14;10(11):. 2021-11-14
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DSS-induced inflammation in the colon drives a proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod
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Journal of Neuroinflammation, 2021-11-10;18(1):263. 2021-11-10
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Complement-containing small extracellular vesicles from adventitial fibroblasts induce pro-inflammatory and metabolic reprogramming in macrophages
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JCI Insight, 2021-11-08;0(0):. 2021-11-08
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Macrophage-mediated RON signaling supports breast cancer growth and progression through modulation of IL-35
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Oncogene, 2021-11-06;0(0):. 2021-11-06
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Reduction of Cell Proliferation by Acute C2H6O Exposure
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Social Isolation Induces Neuroinflammation And Microglia Overactivation, While Dihydromyricetin Prevents And Improves Them
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Research square, 2021-10-01;0(0):. 2021-10-01
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Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
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Scientific Reports, 2021-10-01;11(1):19589. 2021-10-01
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Proteoglycan-4 is an essential regulator of synovial macrophage polarization and inflammatory macrophage joint infiltration
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Arthritis Research & Therapy, 2021-09-14;23(1):241. 2021-09-14
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Signaling through Syk or CARD9 Mediates Species-Specific Anti-Candida Protection in Bone Marrow Chimeric Mice
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MBio, 2021-08-31;12(4):e0160821. 2021-08-31
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Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
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Science Advances, 2021-08-27;7(35):. 2021-08-27
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Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice
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PLoS ONE, 2021-08-12;16(8):e0255997. 2021-08-12
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Recombinant annexin A2 inhibits peripheral leukocyte activation and brain infiltration after traumatic brain injury
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Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK/STAT axis in RAW264.7 cells
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Journal of ethnopharmacology, 2021-08-05;0(0):114493. 2021-08-05
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Humidifier disinfectant, sodium dichloroisocyanurate (NaDCC): assessment of respiratory effects to protect workers' health
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Distinct transcription factor networks control neutrophil-driven inflammation
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Pneumococcal Extracellular Vesicles Modulate Host Immunity
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MBio, 2021-07-13;0(0):e0165721. 2021-07-13
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Conditioned secretome of adipose-derived stem cells improves dextran sulfate sodium-induced colitis in mice
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World Journal of Gastroenterology, 2021-06-21;27(23):3342-3356. 2021-06-21
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Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
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Nature Communications, 2021-06-10;12(1):3543. 2021-06-10
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Polymerized albumin restores impaired hemodynamics in endotoxemia and polymicrobial sepsis
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Scientific Reports, 2021-05-25;11(1):10834. 2021-05-25
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Moderately pathogenic maternal influenza A virus infection disrupts placental integrity but spares the fetal brain
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Brain, Behavior, and Immunity, 2021-05-12;0(0):. 2021-05-12
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KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models
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International Journal of Molecular Sciences, 2021-05-09;22(9):. 2021-05-09
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Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice
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Viruses, 2021-04-22;13(5):. 2021-04-22
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Vaccine delivery alerts innate immune systems for more immunogenic vaccination
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JCI Insight, 2021-04-08;0(0):. 2021-04-08
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JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice
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Scientific Reports, 2021-04-01;11(1):7372. 2021-04-01
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Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36
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Brain, Behavior, and Immunity, 2021-02-13;94(0):437-457. 2021-02-13
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Sulfated polysaccharide directs therapeutic angiogenesis via endogenous VEGF secretion of macrophages
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Science Advances, 2021-02-10;7(7):. 2021-02-10
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The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice
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Mediators of Inflammation, 2021-01-22;2021(0):7372893. 2021-01-22
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Differential expression of Triggering Receptor Expressed on Myeloid cells 2 (Trem2) in tissue eosinophils
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Journal of leukocyte biology, 2021-01-06;0(0):. 2021-01-06
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CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain
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Theranostics, 2021-01-01;11(3):1059-1078. 2021-01-01
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Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation
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Viruses, 2020-12-22;13(1):. 2020-12-22
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Oxysterols present in Alzheimer's disease brain induce synaptotoxicity by activating astrocytes: A major role for lipocalin-2
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Redox Biology, 2020-12-17;39(0):101837. 2020-12-17
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Designed CXCR4 mimic acts as a soluble chemokine receptor that blocks atherogenic inflammation by agonist-specific targeting
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Nat Commun, 2020-11-25;11(1):5981. 2020-11-25
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Calcitriol in the Presence of Conditioned Media from Metastatic Breast Cancer Cells Enhances Ex Vivo Polarization of M2 Alternative Murine Bone Marrow-Derived Macrophages
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Cancers (Basel), 2020-11-23;12(11):. 2020-11-23
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The nSMase2/Smpd3 gene modulates the severity of muscular dystrophy and the emotional stress response in mdx mice
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BMC Med, 2020-11-19;18(1):343. 2020-11-19
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IL-1&beta Induced Cytokine Expression by Spinal Astrocytes Can Play a Role in the Maintenance of Chronic Inflammatory Pain
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Frontiers in Physiology, 2020-11-16;11(0):543331. 2020-11-16
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Loss of hepatocyte cell division leads to liver inflammation and fibrosis
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PLoS Genet, 2020-11-04;16(11):e1009084. 2020-11-04
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Inhibitory effect of ginsenoside Rg3 on cancer stemness and mesenchymal transition in breast cancer via regulation of myeloid-derived suppressor cells
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PLoS ONE, 2020-10-22;15(10):e0240533. 2020-10-22
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The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury
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Mol Brain, 2020-10-20;13(1):143. 2020-10-20
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Onion Bulb Extract Downregulates EGFR/ERK1/2/AKT Signaling Pathway and Synergizes With Steroids to Inhibit Allergic Inflammation
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Front Pharmacol, 2020-10-02;11(0):551683. 2020-10-02
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Suppressing neutrophil-dependent angiogenesis abrogates resistance to anti-VEGF antibody in a genetic model of colorectal cancer
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Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;0(0):. 2020-08-19
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Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI
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Sci Adv, 2020-08-12;6(33):eaaz0748. 2020-08-12
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Antagonists of the serotonin receptor 5A target human breast tumor initiating cells
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BMC Cancer, 2020-08-05;20(1):724. 2020-08-05
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Interleukin 16 contributes to gammaherpesvirus pathogenesis by inhibiting viral reactivation
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PLoS Pathog., 2020-07-31;16(7):e1008701. 2020-07-31
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Cellular Virotherapy Increases Tumor-Infiltrating Lymphocytes (TIL) and Decreases their PD-1+ Subsets in Mouse Immunocompetent Models
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TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis
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Cancers (Basel), 2020-07-08;12(7):. 2020-07-08
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GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models
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Sci Rep, 2020-07-03;10(1):11003. 2020-07-03
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Xenogenization of tumor cells by fusogenic exosomes in tumor microenvironment ignites and propagates antitumor immunity
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Science Advances, 2020-07-01;6(27):. 2020-07-01
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Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling
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Linking skeletal muscle aging with osteoporosis by lamin A/C deficiency
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PLoS Biol., 2020-06-01;18(6):e3000731. 2020-06-01
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Neutrophil extracellular traps exacerbate neurological deficits after traumatic brain injury
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Sci Adv, 2020-05-29;6(22):eaax8847. 2020-05-29
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Assessment of the Immunomodulatory Properties of the Probiotic Strain Lactobacillus paracasei K5 in vitro and In Vivo
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Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
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Nat Commun, 2020-05-01;11(1):2177. 2020-05-01
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Tumor-infiltrating Leukocytes Suppress Local Inflammation Via Interleukin-1 Receptor Antagonist in a Syngeneic Prostate Cancer Model
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Biology (Basel), 2020-03-31;9(4):. 2020-03-31
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Induction of IkappaB? Augments Cytokine and Chemokine Production by IL-33 in Mast Cells
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A novel STAT3 inhibitor attenuates angiotensin II-induced abdominal aortic aneurysm progression in mice through modulating vascular inflammation and autophagy
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Cell Death Dis, 2020-02-18;11(2):131. 2020-02-18
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Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas
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Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer's Disease
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J Clin Med, 2020-02-05;9(2):. 2020-02-05
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LncRNA-AC006129.1 reactivates a SOCS3-mediated anti-inflammatory response through DNA methylation-mediated CIC downregulation in schizophrenia
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Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
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Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-&kappaB-dependent MAPK pathways
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Parasit Vectors, 2020-01-13;13(1):20. 2020-01-13
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Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
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A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease
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Sci Rep, 2019-12-05;9(1):18393. 2019-12-05
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Deletion of the Mir-106b~?25 MicroRNA cluster attenuates atherosclerosis in Apolipoprotein E knockout mice
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Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-alpha and stromal-derived cell factor 1
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Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
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LAG-3 confers poor prognosis and its blockade reshapes antitumor response in head and neck squamous cell carcinoma
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Oncoimmunology, 2016-10-07;5(11):e1239005. 2016-10-07
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Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity
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J. Virol., 2016-09-29;90(20):9263-84. 2016-09-29
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Human and mouse monocytes display distinct signalling and cytokine profiles upon stimulation with FFAR2/FFAR3 short-chain fatty acid receptor agonists
Sci Rep, 2016-09-26;6(0):34145. 2016-09-26
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Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection
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The Thyroid Hormone Receptors Inhibit Hepatic Interleukin-6 Signaling During Endotoxemia
Sci Rep, 2016-08-03;6(0):30990. 2016-08-03
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The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages
PLoS ONE, 2016-07-14;11(7):e0158662. 2016-07-14
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Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
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Sci Rep, 2016-06-22;6(0):28538. 2016-06-22
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Proatherosclerotic Effect of the ?1-Subunit of Soluble Guanylyl Cyclase by Promoting Smooth Muscle Phenotypic Switching
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Am J Pathol, 2016-06-15;0(0):. 2016-06-15
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Impact of the Sensory Neurons on Melanoma Growth In Vivo
PLoS ONE, 2016-05-26;11(5):e0156095. 2016-05-26
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Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
PLoS ONE, 2016-05-25;11(5):e0156151. 2016-05-25
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Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
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Repeated Activation of Lung Invariant NKT Cells Results in Chronic Obstructive Pulmonary Disease-Like Symptoms.
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PLoS ONE, 2016-01-26;11(1):e0147710. 2016-01-26
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Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer
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Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
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Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
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Novel Role of TRPML2 in the Regulation of the Innate Immune Response.
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Plasmacytoid Dendritic Cell Activation and IFN-alpha Production Are Prominent Features of Murine Autoimmune Pancreatitis and Human IgG4-Related Autoimmune Pancreatitis.
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Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease.
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Molecular Mechanisms Mediating Retinal Reactive Gliosis Following Bone Marrow Mesenchymal Stem Cell Transplantation.
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EpCAM Aptamer-siRNA Chimera Targets and Regress Epithelial Cancer.
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mRNA-transfected Dendritic Cells Expressing Polypeptides That Link MHC-I Presentation to Constitutive TLR4 Activation Confer Tumor Immunity.
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Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion.
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Lenalidomide reduces microglial activation and behavioral deficits in a transgenic model of Parkinson's disease.
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MicroRNA-26a/-26b-COX-2-MIP-2 Loop Regulates Allergic Inflammation and Allergic Inflammation-promoted Enhanced Tumorigenic and Metastatic Potential of Cancer Cells.
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Grapefruit-Derived Nanovectors Use an Activated Leukocyte Trafficking Pathway to Deliver Therapeutic Agents to Inflammatory Tumor Sites.
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Cancer Res, 2015-04-16;75(12):2520-9. 2015-04-16
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Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
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Cancer Res, 2015-04-08;75(11):2151-8. 2015-04-08
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Specific medicinal plant polysaccharides effectively enhance the potency of a DC-based vaccine against mouse mammary tumor metastasis.
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PLoS ONE, 2015-03-31;10(3):e0122374. 2015-03-31
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PLoS ONE, 2015-03-30;10(3):e0122060. 2015-03-30
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Anticancer Res, 2015-03-01;35(3):1499-508. 2015-03-01
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Frostbite protection in mice expressing an antifreeze glycoprotein.
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PLoS ONE, 2015-02-25;10(2):e0116562. 2015-02-25
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PLoS ONE, 2015-02-23;10(2):e0115827. 2015-02-23
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Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
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The circadian clock controls sunburn apoptosis and erythema in mouse skin.
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Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
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J Immunol, 2014-11-24;194(1):334-48. 2014-11-24
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The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment.
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Effective treatment of allergic airway inflammation with Helicobacter pylori immunomodulators requires BATF3-dependent dendritic cells and IL-10.
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Proc Natl Acad Sci U S A, 2014-07-29;111(32):11810-5. 2014-07-29
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Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
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BMC Biotechnol, 2014-07-15;14(0):63. 2014-07-15
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Notch-dependent repression of miR-155 in the bone marrow niche regulates hematopoiesis in an NF-kappaB-dependent manner.
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Cell Stem Cell, 2014-07-03;15(1):51-65. 2014-07-03
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Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle.
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Cancer Res, 2014-06-30;74(16):4270-81. 2014-06-30
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HPV16-associated tumors control myeloid cell homeostasis in lymphoid organs, generating a suppressor environment for T cells.
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J Leukoc Biol, 2014-06-26;96(4):619-31. 2014-06-26
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Bone marrow stromal cell-derived exosomes as communicators in drug resistance in multiple myeloma cells.
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Blood, 2014-06-13;124(4):555-66. 2014-06-13
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung injury in mice.
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Sci Rep, 2014-06-06;4(0):5204. 2014-06-06
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Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
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Stem Cells, 2014-05-01;32(5):1208-19. 2014-05-01
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Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
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PLoS ONE, 2014-04-14;9(4):e94793. 2014-04-14
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BMP pathway regulation of and by macrophages.
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PLoS ONE, 2014-04-08;9(4):e94119. 2014-04-08
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The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
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PLoS Pathog, 2014-04-03;10(4):e1004061. 2014-04-03
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Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10.
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Hum Mol Genet, 2014-03-21;23(15):3990-4000. 2014-03-21
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IL-1alpha and complement cooperate in triggering local neutrophilic inflammation in response to adenovirus and eliminating virus-containing cells.
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PLoS Pathog, 2014-03-20;10(3):e1004035. 2014-03-20
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Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
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Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.
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J Virol, 2014-02-26;88(10):5421-36. 2014-02-26
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Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype.
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Effects of insulin resistance on skeletal muscle growth and exercise capacity in type 2 diabetic mouse models.
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Am J Physiol Endocrinol Metab, 2014-01-14;306(6):E592-605. 2014-01-14
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Infection with Leishmania major induces a cellular stress response in macrophages.
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IL-15 deficient tax mice reveal a role for IL-1alpha in tumor immunity.
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PLoS ONE, 2014-01-08;9(1):e85028. 2014-01-08
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A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
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Nat Commun, 2014-01-01;5(0):3175. 2014-01-01
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Concomitant interferon alpha stimulation and TLR3 activation induces neuronal expression of depression-related genes that are elevated in the brain of suicidal persons.
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Novel mechanisms underlying the immediate and transient global tolerization of splenic dendritic cells after vaccination with a self-antigen.
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J Immunol, 2013-12-11;192(2):658-65. 2013-12-11
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Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.
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BMC Cancer, 2013-12-06;13(0):582. 2013-12-06
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Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
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Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10).
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KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
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Circulation, 2013-09-10;128(11):S163-74. 2013-09-10
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Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.
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The bradykinin B1 receptor regulates Abeta deposition and neuroinflammation in Tg-SwDI mice.
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The MSHA Strain of Pseudomonas Aeruginosa Activated TLR Pathway and Enhanced HIV-1 DNA Vaccine Immunoreactivity.
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Interleukin-33 primes mast cells for activation by IgG immune complexes.
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NLRP3 suppresses NK cell-mediated responses to carcinogen-induced tumors and metastases.
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TGFbeta signalling plays an important role in IL4-induced alternative activation of microglia.
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PLoS ONE, 2012-03-30;7(3):e34122. 2012-03-30
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A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
J. Virol., 2012-03-28;86(11):6286-302. 2012-03-28
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The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
Authors: Tran K, Risingsong R, Royce D
Cancer Prev Res (Phila), 2012-03-08;5(5):726-34. 2012-03-08
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The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
PLoS ONE, 2012-02-29;7(2):e32125. 2012-02-29
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Preventing phosphorylation of sterol regulatory element-binding protein 1a by MAP-kinases protects mice from fatty liver and visceral obesity.
Authors: Kotzka J, Knebel B, Haas J, Kremer L, Jacob S, Hartwig S, Nitzgen U, Muller-Wieland D
PLoS ONE, 2012-02-27;7(2):e32609. 2012-02-27
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Liver-specific expression of transcriptionally active SREBP-1c is associated with fatty liver and increased visceral fat mass.
Authors: Knebel B, Haas J, Hartwig S, Jacob S, Kollmer C, Nitzgen U, Muller-Wieland D, Kotzka J
PLoS ONE, 2012-02-21;7(2):e31812. 2012-02-21
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The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.
Authors: Min KJ, Cho KH, Kwon TK
Cell. Signal., 2012-02-10;24(6):1215-21. 2012-02-10
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Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1.
Authors: Wolf A, Beuerlein K, Eckart C, Weiser H, Dickkopf B, Muller H, Sakurai H, Kracht M
PLoS ONE, 2011-12-22;6(12):e29256. 2011-12-22
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Murine bone marrow-derived macrophages differentiated with GM-CSF become foam cells by PI3Kgamma-dependent fluid-phase pinocytosis of native LDL.
Authors: Anzinger JJ, Chang J, Xu Q, Barthwal MK, Bohnacker T, Wymann MP, Kruth HS
J. Lipid Res., 2011-11-04;53(1):34-42. 2011-11-04
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Stromal interaction essential for vascular endothelial growth factor A-induced tumour growth via transforming growth factor-beta signalling.
Authors: Weidenaar AC, Ter Elst A, Kampen KR, Meeuwsen-de Boer TG, de Jonge HJ, Scherpen FJ, den Dunnen WF, Kamps WA, de Bont ES
Br. J. Cancer, 2011-11-01;105(12):1856-63. 2011-11-01
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Attenuation of leukocyte recruitment via CXCR1/2 inhibition stops the progression of PAH in mice with genetic ablation of endothelial BMPR-II.
Authors: Burton VJ, Holmes AM, Ciuclan LI
Blood, 2011-09-07;118(17):4750-8. 2011-09-07
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Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
Authors: Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
Blood, 2011-09-06;118(18):5031-9. 2011-09-06
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Fine-tuning nucleophosmin in macrophage differentiation and activation.
Authors: Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, Jacquel A, Cally R, Manoury B, Vanden Berghe T, Vandenabeele P, Droin N, Solary E
Blood, 2011-08-29;118(17):4694-704. 2011-08-29
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Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates Escherichia coli-induced acute lung injury in mice.
Authors: Kim ES, Chang YS, Choi SJ, Kim JK, Yoo HS, Ahn SY, Sung DK, Kim SY, Park YR, Park WS
Respir. Res., 2011-08-15;12(0):108. 2011-08-15
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Comparative kinetic analyses of gene profiles of naive CD4+ and CD8+ T cells from young and old animals reveal novel age-related alterations.
Authors: Mirza N, Pollock K, Hoelzinger DB, Dominguez AL, Lustgarten J
Aging Cell, 2011-08-07;10(5):853-67. 2011-08-07
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Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models.
Authors: Persson J, Beyer I, Yumul R, Li Z, Kiem HP, Roffler S, Lieber A
PLoS ONE, 2011-07-14;6(7):e22303. 2011-07-14
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TGF-beta limits IL-33 production and promotes the resolution of colitis through regulation of macrophage function.
Authors: Rani R, Smulian AG, Greaves DR, Hogan SP, Herbert DR
Eur. J. Immunol., 2011-05-27;41(7):2000-9. 2011-05-27
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Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation.
Authors: Koroskenyi K, Duro E, Pallai A, Sarang Z, Kloor D, Ucker DS, Beceiro S, Castrillo A, Chawla A, Ledent CA, Fésüs L, Szondy Z
J. Immunol., 2011-05-18;186(12):7144-55. 2011-05-18
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SHIP-deficient mice develop spontaneous intestinal inflammation and arginase-dependent fibrosis.
Authors: McLarren KW, Cole AE, Weisser SB, Voglmaier NS, Conlin VS, Jacobson K, Popescu O, Boucher JL, Sly LM
Am. J. Pathol., 2011-05-07;179(1):180-8. 2011-05-07
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TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
J. Lipid Res., 2011-04-26;52(7):1307-18. 2011-04-26
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Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
Authors: Diao J, Zhao J, Winter E
J. Immunol., 2011-03-23;186(9):5058-67. 2011-03-23
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Reduced VEGF production, angiogenesis, and vascular regrowth contribute to the antitumor properties of dual mTORC1/mTORC2 inhibitors.
Authors: Falcon BL, Barr S, Gokhale PC, Chou J, Fogarty J, Depeille P, Miglarese M, Epstein DM, McDonald DM
Cancer Res., 2011-03-01;71(5):1573-83. 2011-03-01
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HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation.
Authors: Braunstein M, Anderson MK
Mol. Cell. Biol., 2010-12-28;31(5):971-82. 2010-12-28
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Caspase-3 is transiently activated without cell death during early antigen driven expansion of CD8(+) T cells in vivo.
Authors: McComb S, Mulligan R, Sad S
PLoS ONE, 2010-12-22;5(12):e15328. 2010-12-22
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A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling.
Authors: Kennedy DJ, Kuchibhotla S
Cardiovasc. Res., 2010-11-18;0(0):. 2010-11-18
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Tim3 binding to galectin-9 stimulates antimicrobial immunity.
Authors: Jayaraman P, Sada-Ovalle I, Beladi S, Anderson AC, Dardalhon V, Hotta C, Kuchroo VK, Behar SM
J. Exp. Med., 2010-10-11;207(11):2343-54. 2010-10-11
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Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation.
Authors: Cedeno-Laurent F, Barthel SR, Opperman MJ, Lee DM, Clark RA, Dimitroff CJ
J. Immunol., 2010-09-15;185(8):4659-72. 2010-09-15
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Salmonella enterica serovar Typhimurium-induced placental inflammation and not bacterial burden correlates with pathology and fatal maternal disease.
Authors: Chattopadhyay A, Robinson N, Sandhu JK, Finlay BB, Sad S, Krishnan L
Infect. Immun., 2010-03-01;78(5):2292-301. 2010-03-01
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Protective effect of human amniotic fluid stem cells in an immunodeficient mouse model of acute tubular necrosis.
Authors: Perin L, Sedrakyan S, Giuliani S, Da Sacco S, Carraro G, Shiri L, Lemley KV, Rosol M, Wu S, Atala A, Warburton D, De Filippo RE
PLoS ONE, 2010-02-24;5(2):e9357. 2010-02-24
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Small-molecule inhibitors of vascular adhesion protein-1 reduce the accumulation of myeloid cells into tumors and attenuate tumor growth in mice.
Authors: Marttila-Ichihara F, Castermans K, Auvinen K, oude Egbrink MG, Jalkanen S, Griffioen AW, Salmi M
J. Immunol., 2010-02-12;184(6):3164-73. 2010-02-12
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VEGF-A promotes lymphoma tumour growth by activation of STAT proteins and inhibition of p27(KIP1) via paracrine mechanisms.
Authors: Roorda BD, Ter Elst A, Scherpen FJ, Meeuwsen-de Boer TG, Kamps WA, de Bont ES
Eur. J. Cancer, 2010-01-22;46(5):974-82. 2010-01-22
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The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
Authors: Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA
Vaccine, 2010-01-07;28(10):2249-58. 2010-01-07
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Recruitment and differentiation of conventional dendritic cell precursors in tumors.
Authors: Diao J, Zhao J, Winter E, Cattral MS
J. Immunol., 2009-12-21;184(3):1261-7. 2009-12-21
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Paracrine induction of endothelium by tumor exosomes.
Authors: Hood JL, Pan H, Lanza GM, Wickline SA
Lab. Invest., 2009-09-28;89(11):1317-28. 2009-09-28
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Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity.
Authors: McKee AS, Munks MW, MacLeod MK, Fleenor CJ, Van Rooijen N, Kappler JW, Marrack P
J. Immunol., 2009-09-04;183(7):4403-14. 2009-09-04
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Virus binding to a plasma membrane receptor triggers interleukin-1 alpha-mediated proinflammatory macrophage response in vivo.
Authors: Di Paolo NC, Miao EA, Iwakura Y, Murali-Krishna K, Aderem A, Flavell RA, Papayannopoulou T, Shayakhmetov DM
Immunity, 2009-07-02;31(1):110-21. 2009-07-02
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JNK-induced MCP-1 production in spinal cord astrocytes contributes to central sensitization and neuropathic pain.
Authors: Gao YJ, Zhang L, Samad OA, Suter MR, Yasuhiko K, Xu ZZ, Park JY, Lind AL, Ma Q, Ji RR
J. Neurosci., 2009-04-01;29(13):4096-108. 2009-04-01
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Inhibition of the chemokine (C-C motif) ligand 2/chemokine (C-C motif) receptor 2 pathway attenuates hyperglycaemia and inflammation in a mouse model of hepatic steatosis and lipoatrophy.
Authors: Yang SJ, IglayReger HB, Kadouh HC, Bodary PF
Diabetologia, 2009-03-10;52(0):972-81. 2009-03-10
FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
Reviews for Proteome Profiler Mouse Cytokine Array Kit, Panel A
Average Rating: 4.7 (Based on 31 Reviews)
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The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.
Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.
20 ul serum was used to perform the experiment.
mouse serum 200ul used for cancer model
Used serum to see the role of gene in pavreatic cancer
Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.
150UL SERUM WAS USED
serum from knock-out mice
Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!
T cells isolated and stimulated with various agonists.
This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.
High quality results with simple and straightforward protocol. No optimization needed.
instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.
Very sensitive cytokine assay!
performed on cell conditoned media from mouse fibroblasts
Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.
Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.
Bronchoalveolar lavage fluid from C57BL/6J mouse