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Proteome Profiler Mouse Cytokine Array Kit, Panel A

Catalog #: ARY006
379 Citations 31 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
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ARY006
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The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL13/BLC/BCA-1 IL-5 M-CSF
C5a IL-6 CCL2/JE/MCP-1
G-CSF IL-7 CCL12/MCP-5
GM-CSF IL-10 CXCL9/MIG
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
ICAM-1 IL-16 CXCL2/MIP-2
IFN-gamma IL-17 CCL5/RANTES
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha
IL-3 CXCL1/KC TREM-1
IL-4    

 

Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL13/BLC/BCA-1 MCX130 DY470
C5a   DY2150
G-CSF MCS00 DY414
GM-CSF MGM00 DY415
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-16    
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL1/KC MKC00B DY453
M-CSF MMC00 DY416
CCL2/MCP-1/JE MJE00 DY479
CCL12/MCP-5 MCC120 DY428
CXCL9/MIG MCX900 DY492
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.
Species
Mouse

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Scientific Data

N/A The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. View Larger

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

N/A The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. View Larger

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

N/A The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. View Larger

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

379 Citations: Showing 1 - 10
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  1. THBru attenuates diabetic cardiomyopathy by inhibiting RAGE-dependent inflammation
    Authors: Xu, HH;Hao, SX;Sun, HY;Dong, XX;Lin, Y;Lou, H;Zhao, LM;Tang, PP;Dou, ZJ;Han, JJ;Du, MH;Chen, ZX;Kopylov, P;Shchekochikhin, D;Liu, X;Zhang, Y;
    Acta pharmacologica Sinica  2024-06-11
  2. Key features of the innate immune response is mediated by the immunoproteasome in microglia
    Authors: Izadjoo, S;Moritz, KE;Khayrullina, G;Bergman, EM;Melvin, BM;Stinson, MW;Paulson, SG;McCormack, NM;Anderson, KN;Lewis, LA;Rotty, JD;Burnett, BG;
    Research square  2024-06-06
  3. Single-cell analysis identifies PLK1 as a driver of immunosuppressive tumor microenvironment in LUAD
    Authors: Kong, Y;Li, C;Liu, J;Wu, S;Zhang, M;Allison, DB;Hassan, F;He, D;Wang, X;Mao, F;Zhang, Q;Zhang, Y;Li, Z;Wang, C;Liu, X;
    PLoS genetics  2024-06-01
  4. Inhibition of EGFR/ErbB does not protect against C. difficile toxin B
    Authors: Siddiqi, U;Lunnemann, HM;Childress, KO;Shupe, JA;Rutherford, SA;Farrow, MA;Washington, MK;Coffey, RJ;Lacy, DB;Markham, NO;
    bioRxiv : the preprint server for biology  2024-05-14
  5. A non-lipid nucleic acid delivery vector with dendritic cell tropism and stimulation
    Authors: Kim, HS;Halabi, EA;Enbergs, N;Kohler, RH;Fei, F;Garris, CS;Weissleder, R;
    Theranostics  2024-05-05
  6. Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a CCL2-CCR2 pathway and NO dysregulation
    Authors: Ye, Q;Jo, J;Wang, CY;Oh, H;Zhan, J;Choy, TJ;Kim, KI;D'Alessandro, A;Reshetnyak, YK;Jung, SY;Chen, Z;Marrelli, SP;Lee, HK;
    Cell reports  2024-05-05
  7. TLR7-dependent eosinophil degranulation links psoriatic skin inflammation to small intestinal inflammatory changes in mice
    Authors: Kim, HJ;Jang, J;Na, K;Lee, EH;Gu, HJ;Lim, YH;Joo, SA;Baek, SE;Roh, JY;Maeng, HJ;Kim, YH;Lee, YJ;Oh, BC;Jung, Y;
    Experimental & molecular medicine  2024-05-01
  8. Colon impairments and inflammation driven by an altered gut microbiota leads to social behavior deficits rescued by hyaluronic acid and celecoxib
    Authors: Agranyoni, O;Sur, D;Amidror, S;Shidlovsky, N;Bagaev, A;Yissachar, N;Pinhasov, A;Navon-Venezia, S;
    BMC medicine  2024-04-29
  9. Pharmacological expansion of type 2 alveolar epithelial cells promotes regenerative lower airway repair
    Authors: Shao, S;Zhang, N;Specht, GP;You, S;Song, L;Fu, Q;Huang, D;You, H;Shu, J;Domissy, A;Li, S;Nguyen-Tran, V;Joseph, SB;Chatterjee, AK;Chen, JJ;Schultz, PG;Bollong, MJ;
    Proceedings of the National Academy of Sciences of the United States of America  2024-04-16
  10. Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice
    Authors: Zhang, N;Wang, X;Feng, M;Li, M;Wang, J;Yang, H;He, S;Xia, Z;Shang, L;Jiang, X;Sun, M;Wu, Y;Ren, C;Zhang, X;Li, J;Gao, F;
    Nature communications  2024-04-10
  11. Dietary emulsifier polysorbate 80 exposure accelerates age-related cognitive decline
    Authors: Zhang, L;Yin, Z;Liu, X;Jin, G;Wang, Y;He, L;Li, M;Pang, X;Yan, B;Jia, Z;Ma, J;Wei, J;Cheng, F;Li, D;Wang, L;Han, Z;Liu, Q;Chen, F;Cao, H;Lei, P;
    Brain, behavior, and immunity  2024-03-31
  12. Reactive astrocytes secrete the chaperone HSPB1 to mediate neuroprotection
    Authors: Yang, F;Beltran-Lobo, P;Sung, K;Goldrick, C;Croft, CL;Nishimura, A;Hedges, E;Mahiddine, F;Troakes, C;Golde, TE;Perez-Nievas, BG;Hanger, DP;Noble, W;Jimenez-Sanchez, M;
    Science advances  2024-03-22
  13. Nuclear microRNA-mediated transcriptional control determines adult microglial homeostasis and brain function
    Authors: Li, Z;Mao, K;Liu, L;Xu, S;Zeng, M;Fu, Y;Huang, J;Li, T;Gao, G;Teng, ZQ;Sun, Q;Chen, D;Cheng, Y;
    Cell reports  2024-03-13
  14. Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart
    Authors: Radmand, A;Kim, H;Beyersdorf, J;Dobrowolski, CN;Zenhausern, R;Paunovska, K;Huayamares, SG;Hua, X;Han, K;Loughrey, D;Hatit, MZC;Del Cid, A;Ni, H;Shajii, A;Li, A;Muralidharan, A;Peck, HE;Tiegreen, KE;Jia, S;Santangelo, PJ;Dahlman, JE;
    Proceedings of the National Academy of Sciences of the United States of America  2024-03-12
  15. Anti-Inflammatory Effects of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Extract Fermented by Ganoderma applanatum Mycelia
    Authors: Kim, CH;Kwon, YJ;Jang, YA;
    Pharmaceutics  2024-03-05
  16. Plasticity-induced repression of Irf6 underlies acquired resistance to cancer immunotherapy in pancreatic ductal adenocarcinoma
    Authors: Kim, IK;Diamond, MS;Yuan, S;Kemp, SB;Kahn, BM;Li, Q;Lin, JH;Li, J;Norgard, RJ;Thomas, SK;Merolle, M;Katsuda, T;Tobias, JW;Baslan, T;Politi, K;Vonderheide, RH;Stanger, BZ;
    Nature communications  2024-02-20
  17. Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase
    Authors: Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M;
    International immunopharmacology  2024-02-15
  18. CHCHD4-TRIAP1 regulation of innate immune signaling mediates skeletal muscle adaptation to exercise
    Authors: Ma, J;Wang, PY;Zhuang, J;Son, AY;Karius, AK;Syed, AM;Nishi, M;Wu, Z;Mori, MP;Kim, YC;Hwang, PM;
    Cell reports  2023-12-28
  19. Contrasting roles of different mismatch repair proteins in basal-like breast cancer
    Authors: Mo, J;Borcherding, N;Jo, S;Tithi, TI;Cho, E;Cash, KE;Honda, M;Wang, L;Ahmed, KK;Weigel, R;Spies, M;Kolb, R;Zhang, W;
    bioRxiv : the preprint server for biology  2023-09-13
  20. Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer
    Authors: Fan, YC;Fong, YC;Kuo, CT;Li, CW;Chen, WY;Lin, JD;Bürtin, F;Linnebacher, M;Bui, QT;Lee, KD;Tsai, YC;
    Cell & bioscience  2023-08-10
  21. Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling
    Authors: Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G;
    Respiratory research  2023-07-13
  22. An LRP1-binding motif in cellular prion protein replicates cell-signaling activities of the full-length protein
    Authors: Mantuano, E;Zampieri, C;Azmoon, P;Gunner, CB;Heye, KR;Gonias, SL;
    JCI insight  2023-06-27
  23. Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection
    Authors: Fukushi, M;Ohsawa, R;Okinaka, Y;Oikawa, D;Kiyono, T;Moriwaki, M;Irie, T;Oda, K;Kamei, Y;Tokunaga, F;Sotomaru, Y;Maruyama, H;Kawakami, H;Sakaguchi, T;
    PloS one  2023-06-23
  24. Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth
    Authors: Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz
    iScience  2023-06-16
  25. MPC-n (IgG) improves long-term cognitive impairment in the mouse model of repetitive mild traumatic brain injury
    Authors: Zhang, C;Wei, C;Huang, X;Hou, C;Liu, C;Zhang, S;Zhao, Z;Liu, Y;Zhang, R;Zhou, L;Li, Y;Yuan, X;Zhang, J;
    BMC medicine  2023-05-30
  26. Tumour extracellular vesicles and particles induce liver metabolic dysfunction
    Authors: Wang, G;Li, J;Bojmar, L;Chen, H;Li, Z;Tobias, GC;Hu, M;Homan, EA;Lucotti, S;Zhao, F;Posada, V;Oxley, PR;Cioffi, M;Kim, HS;Wang, H;Lauritzen, P;Boudreau, N;Shi, Z;Burd, CE;Zippin, JH;Lo, JC;Pitt, GS;Hernandez, J;Zambirinis, CP;Hollingsworth, MA;Grandgenett, PM;Jain, M;Batra, SK;DiMaio, DJ;Grem, JL;Klute, KA;Trippett, TM;Egeblad, M;Paul, D;Bromberg, J;Kelsen, D;Rajasekhar, VK;Healey, JH;Matei, IR;Jarnagin, WR;Schwartz, RE;Zhang, H;Lyden, D;
    Nature  2023-05-24
  27. Progerinin, an Inhibitor of Progerin, Alleviates Cardiac Abnormalities in a Model Mouse of Hutchinson-Gilford Progeria Syndrome
    Authors: Kang, SM;Seo, S;Song, EJ;Kweon, O;Jo, AH;Park, S;Woo, TG;Kim, BH;Oh, GT;Park, BJ;
    Cells  2023-04-24
  28. Spatiotemporal Cofilin Signaling, Microglial Activation, Neuroinflammation, and Cognitive Impairment Following Hemorrhagic Brain Injury
    Authors: Almarghalani, DA;Sha, X;Mrak, RE;Shah, ZA;
    Cells  2023-04-13
  29. Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway
    Authors: Q Ye, J Jo, CY Wang, H Oh, TJ Choy, K Kim, A Dâ Alessan, YK Reshetnyak, SY Jung, Z Chen, SP Marrelli, HK Lee
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.  2023-04-03
  30. Correction of haemorrhagic shock-associated coagulopathy and impaired haemostasis by plasma, prothrombin complex concentrates or an activated protein C-targeted DNA aptamer in mice
    Authors: LJ Eltringham, SC Meixner, ELG Pryzdial, WP Sheffield
    Scientific Reports, 2023-03-07;13(1):3811.  2023-03-07
  31. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy
    Authors: X Bai, ZQ Guo, YP Zhang, ZZ Fan, LJ Liu, L Liu, LL Long, SC Ma, J Wang, Y Fang, XR Tang, YJ Zeng, X Pan, DH Wu, ZY Dong
    Nature Communications, 2023-03-04;14(1):1247.  2023-03-04
  32. Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice
    Authors: M Tfilin, N Gobshtis, D Fozailoff, VE Fraifeld, G Turgeman
    International Journal of Molecular Sciences, 2023-02-24;24(5):.  2023-02-24
  33. Hypoxia alters the immune response in mouse peritoneal macrophages infected with influenza a virus with truncated NS1 protein
    Authors: M Hancková, L Miháliková, S Pastorekov, T Betáková
    Cytokine, 2023-02-14;164(0):156138.  2023-02-14
  34. CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancer
    Authors: JM Houthuijze, R de Bruijn, E van der Bu, AP Drenth, E Wientjens, T Filipovic, E Bullock, CS Brambillas, EM Pulver, M Nieuwland, I de Rink, F van Diepen, S Klarenbeek, R Kerkhoven, VG Brunton, CLGJ Scheele, MC Boelens, J Jonkers
    Nature Communications, 2023-01-12;14(1):183.  2023-01-12
  35. TGFbeta1+CCR5+ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice
    Authors: J Li, Z Yao, X Liu, R Duan, X Yi, A Ayoub, JO Sanders, A Mesfin, L Xing, BF Boyce
    Nature Communications, 2023-01-11;14(1):159.  2023-01-11
  36. Disruption of CCL2 in Mesenchymal Stem Cells as an Anti-Tumor Approach against Prostate Cancer
    Authors: QT Bui, KD Lee, YC Fan, BS Lewis, LW Deng, YC Tsai
    Cancers, 2023-01-10;15(2):.  2023-01-10
  37. Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer
    Authors: EY Faraoni, BJ O'Brien, LN Strickland, BK Osborn, V Mota, J Chaney, CL Atkins, P Cen, J Rowe, J Cardenas, KL Poulsen, CJ Wray, NC Thosani, JM Bailey-Lun
    Cancer Immunology Research, 2023-01-03;11(1):4-12.  2023-01-03
  38. CDK4/6 inhibition triggers ICAM1-driven immune response and sensitizes LKB1 mutant lung cancer to immunotherapy
    Authors: X Bai, ZQ Guo, YP Zhang, ZZ Fan, LJ Liu, L Liu, LL Long, SC Ma, J Wang, Y Fang, XR Tang, YJ Zeng, X Pan, DH Wu, ZY Dong
    Nature Communications, 2023;14(1):1247.  2023
  39. KLK6/PAR1 Axis Promotes Tumor Growth and Metastasis by Regulating Cross-Talk between Tumor Cells and Macrophages
    Authors: YS Hwang, HJ Cho, ES Park, J Lim, HR Yoon, JT Kim, SR Yoon, H Jung, YK Choe, YH Kim, CH Lee, YT Kwon, BY Kim, HG Lee
    Cells, 2022-12-16;11(24):.  2022-12-16
  40. Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life
    Authors: S Pandey, M Mahato, P Srinath, U Bhutani, TJ Goap, P Ravipati, PK Vemula
    Nature Communications, 2022-12-01;13(1):7394.  2022-12-01
  41. Neuronal deletion of MnSOD in mice leads to demyelination, inflammation and progressive paralysis that mimics phenotypes associated with progressive multiple sclerosis
    Authors: S Bhaskaran, G Kumar, N Thadathil, KM Piekarz, S Mohammed, SD Lopez, R Qaisar, D Walton, JL Brown, A Murphy, N Smith, D Saunders, MJ Beckstead, S Plafker, TL Lewis, R Towner, SS Deepa, A Richardson, RC Axtell, H Van Remmen
    Redox Biology, 2022-11-26;59(0):102550.  2022-11-26
  42. Oleuropein suppresses endometriosis progression and improves the fertility of mice with endometriosis
    Authors: Y Park, YJ Cho, N Sung, MJ Park, X Guan, WE Gibbons, BW O'Malley, SJ Han
    Journal of Biomedical Science, 2022-11-22;29(1):100.  2022-11-22
  43. Involvement of the p38 MAPK-NLRC4-Caspase-1 Pathway in Ionizing Radiation-Enhanced Macrophage IL-1beta Production
    Authors: JS Baik, YN Seo, YC Lee, JM Yi, MH Rhee, MT Park, SD Kim
    International Journal of Molecular Sciences, 2022-11-09;23(22):.  2022-11-09
  44. Systemic checkpoint blockade by PD-L1 single-chain antibody confers potent anti-tumor immunity and long-term survival
    Authors: H Wang, V Khattar, JA Hensel, R Ashton, Y Lu, AG Sorace, Y Wang, JS Deshane, JL Mieher, C Deivanayag, S Ponnazhaga
    Molecular Cancer Therapeutics, 2022-11-03;0(0):.  2022-11-03
  45. Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal anti-inflammatory AKT signaling
    Authors: EY Lee, SM Kim, JH Hwang, SY Jang, S Park, S Choi, GS Lee, J Hwang, JH Moon, PL Fox, S Kim, CH Lee, MH Kim
    Nature Communications, 2022-10-29;13(1):6455.  2022-10-29
  46. AhR Mediated Activation of Pro-Inflammatory Response of RAW 264.7 Cells Modulate the Epithelial-Mesenchymal Transition
    Authors: P Selvam, CM Cheng, HU Dahms, VK Ponnusamy, YY Sun
    Toxics, 2022-10-27;10(11):.  2022-10-27
  47. Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease model
    Authors: K Tange, S Yagi, E Takeshita, M Abe, Y Yamamoto, H Tomida, T Kawamura, M Hanayama, B Matsuura, Y Ikeda, Y Hiasa
    Scientific Reports, 2022-10-26;12(1):17983.  2022-10-26
  48. Functional Complementation of Anti-Adipogenic Phytonutrients for Obesity Prevention and Management
    Authors: Y Urasaki, TT Le
    Nutrients, 2022-10-16;14(20):.  2022-10-16
  49. Nanoparticle-based modulation of CD4+ T cell effector and helper functions enhances adoptive immunotherapy
    Authors: A Isser, AB Silver, HC Pruitt, M Mass, EH Elias, G Aihara, SS Kang, N Bachmann, YY Chen, EK Leonard, JG Bieler, W Chaisawang, J Choy, SR Shannon, S Gerecht, JS Weber, JB Spangler, JP Schneck
    Nature Communications, 2022-10-14;13(1):6086.  2022-10-14
  50. OGG1 Inhibition Reduces Acinar Cell Injury in a Mouse Model of Acute Pancreatitis
    Authors: Z Hajnády, M Nagy-Pénze, MA Demény, K Kovács, T El-Hamoly, J Maléth, P Hegyi, Z Polgár, C Heged?s, L Virág
    Biomedicines, 2022-10-12;10(10):.  2022-10-12
  51. Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy
    Authors: ZM Howard, CK Gomatam, CP Rabolli, J Lowe, AB Piepho, SS Bansal, F Accornero, JA Rafael-For
    JCI Insight, 2022-10-10;0(0):.  2022-10-10
  52. Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses
    Authors: Q Wang, HY Li, ZM Ling, G Chen, ZY Wei
    Journal of Neuroinflammation, 2022-10-04;19(1):244.  2022-10-04
  53. Arginine methylation of integrin alpha-4 prevents fibrosis development in alcohol-associated liver disease
    Authors: M Schonfeld, MT Villar, A Artigues, SA Weinman, I Tikhanovic
    Cellular and Molecular Gastroenterology and Hepatology, 2022-09-30;0(0):.  2022-09-30
  54. FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation
    Authors: W Cai, M Hu, C Li, R Wu, D Lu, C Xie, W Zhang, T Li, S Shen, H Huang, W Qiu, Q Liu, Y Lu, Z Lu
    Autophagy, 2022-09-28;0(0):1-20.  2022-09-28
  55. NIL10: A New IL10-Receptor Binding Nanoparticle That Induces Cardiac Protection in Mice and Pigs Subjected to Acute Myocardial Infarction through STAT3/NF-kappaB Activation
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  303. Bone marrow stromal cell-derived exosomes as communicators in drug resistance in multiple myeloma cells.
    Authors: Wang J, Hendrix A, Hernot S, Lemaire M, De Bruyne E, Van Valckenborgh E, Lahoutte T, De Wever O, Vanderkerken K, Menu E
    Blood, 2014-06-13;124(4):555-66.  2014-06-13
  304. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung injury in mice.
    Authors: Takaoka, Yuki, Goto, Shigeru, Nakano, Toshiaki, Tseng, Hui-Peng, Yang, Shih-Min, Kawamoto, Seiji, Ono, Kazuhisa, Chen, Chao-Lon
    Sci Rep, 2014-06-06;4(0):5204.  2014-06-06
  305. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014-05-01;32(5):1208-19.  2014-05-01
  306. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
    Authors: Zhang, Hanwei, Chin, Arnold I
    PLoS ONE, 2014-04-14;9(4):e94793.  2014-04-14
  307. BMP pathway regulation of and by macrophages.
    Authors: Talati M, West J, Zaynagetdinov R, Hong C, Han W, Blackwell T, Robinson L, Blackwell T, Lane K
    PLoS ONE, 2014-04-08;9(4):e94119.  2014-04-08
  308. The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
    Authors: Hall, Belinda, Hill, Kirsti, McKenna, Michael, Ogbechi, Joy, High, Stephen, Willis, Anne E, Simmonds, Rachel E
    PLoS Pathog, 2014-04-03;10(4):e1004061.  2014-04-03
  309. Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10.
    Authors: Nitahara-Kasahara Y, Hayashita-Kinoh H, Chiyo T, Nishiyama A, Okada H, Takeda S, Okada T
    Hum Mol Genet, 2014-03-21;23(15):3990-4000.  2014-03-21
  310. IL-1alpha and complement cooperate in triggering local neutrophilic inflammation in response to adenovirus and eliminating virus-containing cells.
    Authors: Di Paolo N, Baldwin L, Irons E, Papayannopoulou T, Tomlinson S, Shayakhmetov D
    PLoS Pathog, 2014-03-20;10(3):e1004035.  2014-03-20
  311. Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
    Authors: Gruenwald K, Castagnola P, Besio R, Dimori M, Chen Y, Akel N, Swain F, Skinner R, Eyre D, Gaddy D, Suva L, Morello R
    J Bone Miner Res, 2014-03-01;29(3):666-75.  2014-03-01
  312. Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.
    Authors: Reynaud J, Jegou J, Welsch J, Horvat B
    J Virol, 2014-02-26;88(10):5421-36.  2014-02-26
  313. Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype.
    Authors: Freemerman A, Johnson A, Sacks G, Milner J, Kirk E, Troester M, Macintyre A, Goraksha-Hicks P, Rathmell J, Makowski L
    J Biol Chem, 2014-02-03;289(11):7884-96.  2014-02-03
  314. Effects of insulin resistance on skeletal muscle growth and exercise capacity in type 2 diabetic mouse models.
    Authors: Ostler, Joseph E, Maurya, Santosh, Dials, Justin, Roof, Steve R, Devor, Steven T, Ziolo, Mark T, Periasamy, Muthu
    Am J Physiol Endocrinol Metab, 2014-01-14;306(6):E592-605.  2014-01-14
  315. Infection with Leishmania major induces a cellular stress response in macrophages.
    Authors: Filardy A, Costa-da-Silva A, Koeller C, Guimaraes-Pinto K, Ribeiro-Gomes F, Lopes M, Heise N, Freire-de-Lima C, Nunes M, DosReis G
    PLoS ONE, 2014-01-09;9(1):e85715.  2014-01-09
  316. IL-15 deficient tax mice reveal a role for IL-1alpha in tumor immunity.
    Authors: Rauch D, Harding J, Ratner L
    PLoS ONE, 2014-01-08;9(1):e85028.  2014-01-08
  317. A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
    Authors: Ruiz E, Oeztuerk-Winder F, Ventura J
    Nat Commun, 2014-01-01;5(0):3175.  2014-01-01
  318. Concomitant interferon alpha stimulation and TLR3 activation induces neuronal expression of depression-related genes that are elevated in the brain of suicidal persons.
    Authors: Hoyo-Becerra, Carolina, Huebener, Anastasi, Trippler, Martin, Lutterbeck, Melanie, Liu, Zijian J, Truebner, Kurt, Bajanowski, Thomas, Gerken, Guido, Hermann, Dirk M, Schlaak, Joerg F
    PLoS ONE, 2013-12-31;8(12):e83149.  2013-12-31
  319. Novel mechanisms underlying the immediate and transient global tolerization of splenic dendritic cells after vaccination with a self-antigen.
    Authors: Farkas A, Finn O
    J Immunol, 2013-12-11;192(2):658-65.  2013-12-11
  320. Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.
    Authors: Nie Y, DU L, Mou Y, Xu Z, Weng L, Du Y, Zhu Y, Hou Y, Wang T
    BMC Cancer, 2013-12-06;13(0):582.  2013-12-06
  321. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
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  322. Regulation of adult hematopoiesis by the a disintegrin and metalloproteinase 10 (ADAM10).
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  323. KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
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  324. Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.
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  325. A combinatorial F box protein directed pathway controls TRAF adaptor stability to regulate inflammation.
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  326. The bradykinin B1 receptor regulates Abeta deposition and neuroinflammation in Tg-SwDI mice.
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  327. Blockade of NOX2 and STIM1 signaling limits lipopolysaccharide-induced vascular inflammation.
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    J Clin Invest, 2013-01-25;123(2):887-902.  2013-01-25
  328. The MSHA Strain of Pseudomonas Aeruginosa Activated TLR Pathway and Enhanced HIV-1 DNA Vaccine Immunoreactivity.
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  329. Interleukin-33 primes mast cells for activation by IgG immune complexes.
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  330. NLRP3 suppresses NK cell-mediated responses to carcinogen-induced tumors and metastases.
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    Cancer Res, 2012-09-17;72(22):5721-32.  2012-09-17
  331. TGFbeta signalling plays an important role in IL4-induced alternative activation of microglia.
    J Neuroinflammation, 2012-09-04;9(0):210.  2012-09-04
  332. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012-07-30;189(5):2545-52.  2012-07-30
  333. Exosomes isolated from mycobacteria-infected mice or cultured macrophages can recruit and activate immune cells in vitro and in vivo.
    Authors: Singh PP, Smith VL, Karakousis PC, Schorey JS
    J. Immunol., 2012-06-20;189(2):777-85.  2012-06-20
  334. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012-06-13;5(0):702.  2012-06-13
  335. Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis.
    Authors: Serezani CH, Kane S, Collins L
    J. Immunol., 2012-06-13;189(2):906-15.  2012-06-13
  336. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
    Acta Pharmacol. Sin., 2012-06-11;33(9):1204-16.  2012-06-11
  337. Histone Deacetylase 3 Mediates Allergic Skin Inflammation by Regulating Expression of MCP1 Protein.
    Authors: Kim Y, Kim K, Park D, Lee E, Lee H, Lee YS, Choe J, Jeoung D
    J. Biol. Chem., 2012-06-07;287(31):25844-59.  2012-06-07
  338. Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice.
    J. Neurochem., 2012-06-06;233(0):456-469.  2012-06-06
  339. Pten loss in CD4 T cells enhances their helper function but does not lead to autoimmunity or lymphoma.
    Authors: Soond DR, Garcon F, Patton DT, Rolf J, Turner M, Scudamore C, Garden OA, Okkenhaug K
    J. Immunol., 2012-05-18;188(12):5935-43.  2012-05-18
  340. Early-outgrowth bone marrow cells attenuate renal injury and dysfunction via an antioxidant effect in a mouse model of type 2 diabetes.
    Diabetes, 2012-05-17;61(8):2114-25.  2012-05-17
  341. Clusterin induces the secretion of TNF-α and the chemotactic migration of macrophages.
    Authors: Shim YJ, Kang BH, Choi BK, Park IS, Min BH
    Biochem. Biophys. Res. Commun., 2012-05-07;422(1):200-5.  2012-05-07
  342. Dietary blue pigments derived from genipin, attenuate inflammation by inhibiting LPS-induced iNOS and COX-2 expression via the NF-kappaB inactivation.
    Authors: Wang QS, Xiang Y, Cui YL, Lin KM, Zhang XF
    PLoS ONE, 2012-03-30;7(3):e34122.  2012-03-30
  343. A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
    Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
    J. Virol., 2012-03-28;86(11):6286-302.  2012-03-28
  344. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
    Authors: Tran K, Risingsong R, Royce D
    Cancer Prev Res (Phila), 2012-03-08;5(5):726-34.  2012-03-08
  345. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012-02-29;7(2):e32125.  2012-02-29
  346. Preventing phosphorylation of sterol regulatory element-binding protein 1a by MAP-kinases protects mice from fatty liver and visceral obesity.
    Authors: Kotzka J, Knebel B, Haas J, Kremer L, Jacob S, Hartwig S, Nitzgen U, Muller-Wieland D
    PLoS ONE, 2012-02-27;7(2):e32609.  2012-02-27
  347. Liver-specific expression of transcriptionally active SREBP-1c is associated with fatty liver and increased visceral fat mass.
    Authors: Knebel B, Haas J, Hartwig S, Jacob S, Kollmer C, Nitzgen U, Muller-Wieland D, Kotzka J
    PLoS ONE, 2012-02-21;7(2):e31812.  2012-02-21
  348. The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.
    Authors: Min KJ, Cho KH, Kwon TK
    Cell. Signal., 2012-02-10;24(6):1215-21.  2012-02-10
  349. Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1.
    Authors: Wolf A, Beuerlein K, Eckart C, Weiser H, Dickkopf B, Muller H, Sakurai H, Kracht M
    PLoS ONE, 2011-12-22;6(12):e29256.  2011-12-22
  350. Murine bone marrow-derived macrophages differentiated with GM-CSF become foam cells by PI3Kgamma-dependent fluid-phase pinocytosis of native LDL.
    Authors: Anzinger JJ, Chang J, Xu Q, Barthwal MK, Bohnacker T, Wymann MP, Kruth HS
    J. Lipid Res., 2011-11-04;53(1):34-42.  2011-11-04
  351. Stromal interaction essential for vascular endothelial growth factor A-induced tumour growth via transforming growth factor-beta signalling.
    Authors: Weidenaar AC, Ter Elst A, Kampen KR, Meeuwsen-de Boer TG, de Jonge HJ, Scherpen FJ, den Dunnen WF, Kamps WA, de Bont ES
    Br. J. Cancer, 2011-11-01;105(12):1856-63.  2011-11-01
  352. Attenuation of leukocyte recruitment via CXCR1/2 inhibition stops the progression of PAH in mice with genetic ablation of endothelial BMPR-II.
    Authors: Burton VJ, Holmes AM, Ciuclan LI
    Blood, 2011-09-07;118(17):4750-8.  2011-09-07
  353. Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
    Authors: Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
    Blood, 2011-09-06;118(18):5031-9.  2011-09-06
  354. Fine-tuning nucleophosmin in macrophage differentiation and activation.
    Authors: Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, Jacquel A, Cally R, Manoury B, Vanden Berghe T, Vandenabeele P, Droin N, Solary E
    Blood, 2011-08-29;118(17):4694-704.  2011-08-29
  355. Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates Escherichia coli-induced acute lung injury in mice.
    Authors: Kim ES, Chang YS, Choi SJ, Kim JK, Yoo HS, Ahn SY, Sung DK, Kim SY, Park YR, Park WS
    Respir. Res., 2011-08-15;12(0):108.  2011-08-15
  356. Comparative kinetic analyses of gene profiles of naive CD4+ and CD8+ T cells from young and old animals reveal novel age-related alterations.
    Authors: Mirza N, Pollock K, Hoelzinger DB, Dominguez AL, Lustgarten J
    Aging Cell, 2011-08-07;10(5):853-67.  2011-08-07
  357. Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models.
    Authors: Persson J, Beyer I, Yumul R, Li Z, Kiem HP, Roffler S, Lieber A
    PLoS ONE, 2011-07-14;6(7):e22303.  2011-07-14
  358. TGF-beta limits IL-33 production and promotes the resolution of colitis through regulation of macrophage function.
    Authors: Rani R, Smulian AG, Greaves DR, Hogan SP, Herbert DR
    Eur. J. Immunol., 2011-05-27;41(7):2000-9.  2011-05-27
  359. Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation.
    Authors: Koroskenyi K, Duro E, Pallai A, Sarang Z, Kloor D, Ucker DS, Beceiro S, Castrillo A, Chawla A, Ledent CA, Fésüs L, Szondy Z
    J. Immunol., 2011-05-18;186(12):7144-55.  2011-05-18
  360. SHIP-deficient mice develop spontaneous intestinal inflammation and arginase-dependent fibrosis.
    Authors: McLarren KW, Cole AE, Weisser SB, Voglmaier NS, Conlin VS, Jacobson K, Popescu O, Boucher JL, Sly LM
    Am. J. Pathol., 2011-05-07;179(1):180-8.  2011-05-07
  361. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011-04-26;52(7):1307-18.  2011-04-26
  362. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011-03-23;186(9):5058-67.  2011-03-23
  363. Reduced VEGF production, angiogenesis, and vascular regrowth contribute to the antitumor properties of dual mTORC1/mTORC2 inhibitors.
    Authors: Falcon BL, Barr S, Gokhale PC, Chou J, Fogarty J, Depeille P, Miglarese M, Epstein DM, McDonald DM
    Cancer Res., 2011-03-01;71(5):1573-83.  2011-03-01
  364. HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation.
    Authors: Braunstein M, Anderson MK
    Mol. Cell. Biol., 2010-12-28;31(5):971-82.  2010-12-28
  365. Caspase-3 is transiently activated without cell death during early antigen driven expansion of CD8(+) T cells in vivo.
    Authors: McComb S, Mulligan R, Sad S
    PLoS ONE, 2010-12-22;5(12):e15328.  2010-12-22
  366. A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling.
    Authors: Kennedy DJ, Kuchibhotla S
    Cardiovasc. Res., 2010-11-18;0(0):.  2010-11-18
  367. Tim3 binding to galectin-9 stimulates antimicrobial immunity.
    Authors: Jayaraman P, Sada-Ovalle I, Beladi S, Anderson AC, Dardalhon V, Hotta C, Kuchroo VK, Behar SM
    J. Exp. Med., 2010-10-11;207(11):2343-54.  2010-10-11
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/01/2022
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 10/01/2021

The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 09/23/2021

Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021

20 ul serum was used to perform the experiment.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/31/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anil Singh on 08/25/2021

mouse serum 200ul used for cancer model

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Gaurav Kumar on 08/17/2021

Used serum to see the role of gene in pavreatic cancer

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Allison Scarpitti on 05/10/2021

Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/06/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By jinxiu PAN on 02/24/2020

150UL SERUM WAS USED

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

mouse tissues

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

serum from knock-out mice

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019

T cells isolated and stimulated with various agonists.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019

good!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 03/13/2019

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Brian Englar on 02/07/2019
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 12/11/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018

High quality results with simple and straightforward protocol. No optimization needed.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Adam Guess on 10/20/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017

Very sensitive cytokine assay!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Michelle Chen on 05/10/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Camila Idelí Morales Fénero on 03/15/2017

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017

Bronchoalveolar lavage fluid from C57BL/6J mouse

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Kihong Lim on 09/29/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006