Human Soluble Receptor Array Kit Hematopoietic Panel

• Human sReceptor Array Kit Hematopoietic Panel Ordering Information

Introduction

R&D Systems Human Soluble Receptor Array Kit Hematopoietic Panel (Catalog # ARY011) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 105 different soluble receptors expressed and released by hematopoietic cells in a single sample. No specialized equipment is necessary.

Sample Data

• Product Insert
• Troubleshooting Guide
• Common assays for analytes represented in the Human Common Analyte Array
• Assays for analytes represented in the Human sReceptor Array Kit Hematopoietic Panel

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 2 Hematopoeitic Array Membranes
• 2 Common Analyte Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product datasheet.