Human Pluripotent Stem Cell Antibody Array

• Human Pluripotent Stem Cell Antibody Array Ordering Information

Introduction

The Human Pluripotent Stem Cell Antibody Array is a rapid, sensitive, and economical tool to simultaneously detect the relative expression of 15 pluripotent stem cell markers in a single sample. No specialized equipment is necessary.

• Product Insert (PDF)
• Troubleshooting Guide
• Assays for analytes represented in the Human Pluripotent Stem Cell Array Kit
• Antibodies for analytes related to the Human Pluripotent Stem Cell Array Kit

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

• 8 Array Membranes
• 8-well Multi-dish
• Array Buffers
• Lysis Buffer
• Wash Buffer
• Detection Antibody Cocktail
• Streptavidin-HRP
• Chemiluminescent Detection Reagents
• Transparency Overlay Template
• Detailed Protocol

The Human Pluripotent Stem Cell Array detects multiple stem cell markers in differentiated BG01V cell extracts. Arrays were incubated with 200 µg of each cell extract shown above. Array images shown were collected from 3 minute exposures to X-ray film. Labels on the films correspond to the same proteins quantified on the bar chart.

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.