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Human C-Reactive Protein/CRP DuoSet ELISA

Catalog #: DY1707
66 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1707
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human C-Reactive Protein / CRP ELISA Standard Curve
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Product Details
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Citations (66)
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Supplemental Products
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human C-Reactive Protein/CRP DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human C-Reactive Protein/CRP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human C-Reactive Protein / CRP ELISA Standard Curve View Larger

Human C-Reactive Protein / CRP ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C-Reactive Protein/CRP

C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a sensor and activator of the innate immune response (1). In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation (2). In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a 110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa) sequence identity with mouse and rat CRP, respectively (5). 

CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9, 10). It subsequently enhances the phagocytosis of these opsonized cells (6, 8-10). CRP additionally binds several proteins in the complement cascade including C1q, C4BP, and Factor H (8, 11-13). It enhances activation of the classical complement pathway and the deposition of C3b (9). In later stages of the response, CRP inhibits complement-mediated cell lysis through its binding to C4BP and Factor H (8, 12). These interactions induce the upregulation of complement inhibitory proteins CD46, CD59, and CD55/DAF and inhibit assembly of the membrane attack complex (MAC) (8, 14). 
CRP binds to Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells (15-17), and Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6, 10, 18). CRP binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and dampens the inflammatory response (15, 19). CRP additionally promotes dendritic cell maturation and humoral immunity (10). In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in coronary artery infarction, and inhibits the repair of injured vascular endothelium (7, 19, 20). 

Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human C-Reactive Protein/CRP DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis
    Authors: Li, W;Xia, Y;Yang, J;Sanyal, AJ;Shah, VH;Chalasani, NP;Yu, Q;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: Plasma
  2. Inflammation and autoimmunity are interrelated in patients with sickle cell disease at a steady-state condition: implications for vaso-occlusive crisis, pain, and sensory sensitivity
    Authors: Li, W;Pucka, AQ;Debats, C;Reyes, B;Syed, F;O'Brien, AR;Mehta, R;Manchanda, N;Jacob, SA;Hardesty, BM;Greist, A;Harte, SE;Harris, RE;Yu, Q;Wang, Y;
    medRxiv : the preprint server for health sciences
    Species: Human
    Sample Types: Plasma
  3. Changes in the Proteome of Platelets from Patients with Critical Progression of COVID-19
    Authors: Wolny, M;Rozanova, S;Knabbe, C;Pfeiffer, K;Barkovits, K;Marcus, K;Birschmann, I;
    Cells
    Species: Human
    Sample Types: Cell Lysates, Plasma
  4. Haemoglobin diagnostic cut-offs for anaemia in Indian women of reproductive age
    Authors: Ghosh, S;Palika, R;Dasi, T;Varshney, RK;Parasannanavar, DJ;Sen Gupta, S;Chitikineni, A;Banjara, SK;Pullakhandam, R;Thomas, T;Sachdev, HS;Kurpad, AV;Kulkarni, B;
    European journal of clinical nutrition
    Species: Human
    Sample Types: Serum
  5. Impact of airway challenges on cardiovascular risk in asthma - a randomized controlled trial
    Authors: Moore, LE;Brotto, AR;Fuhr, DP;Rosychuk, RJ;Wong, E;Bhutani, M;Stickland, MK;
    PloS one
    Species: Human
    Sample Types: Serum
  6. Pre-pregnancy obesity is associated with greater systemic inflammation and increased risk of antenatal depression
    Authors: Sominsky, L;O'Hely, M;Drummond, K;Cao, S;Collier, F;Dhar, P;Loughman, A;Dawson, S;Tang, ML;Mansell, T;Saffery, R;Burgner, D;Ponsonby, AL;Vuillermin, P;Barwon Infant Study Investigator Group, ;
    Brain, behavior, and immunity
    Species: Human
    Sample Types: Serum
  7. Central and Peripheral Inflammation in Mild Cognitive Impairment in the Context of Alzheimer's Disease
    Authors: Schmidt-Morgenroth, I;Michaud, P;Gasparini, F;Avrameas, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: CSF, Serum
  8. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), somatic and fatigue symptoms in cardiovascular diseases comorbid major depressive disorder (MDD): A randomized controlled trial
    Authors: Chang, JP;Chang, SS;Chen, HT;Chien, YC;Yang, HT;Huang, SY;Tseng, PT;Chang, CH;Galecki, P;Su, KP;
    Brain, behavior, and immunity
    Species: Human
    Sample Types: Plasma
  9. Efficacy of Cooling Centers for Mitigating Physiological Strain in Older Adults during Daylong Heat Exposure: A Laboratory-Based Heat Wave Simulation
    Authors: Meade, RD;Notley, SR;Akerman, AP;McCormick, JJ;King, KE;Sigal, RJ;Kenny, GP;
    Environmental health perspectives
    Species: Human
    Sample Types: Serum
  10. Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators in overweight women suggest a state of subclinical endotoxemia
    Authors: Metz, CN;Xue, X;Chatterjee, PK;Adelson, R;Brines, M;Tracey, KJ;Gregersen, PK;Pavlov, VA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Plasma
  11. Prognostic accuracy of biomarkers of immune and endothelial activation in Mozambican children hospitalized with pneumonia
    Authors: N Balanza, C Erice, M Ngai, CR McDonald, AM Weckman, J Wright, M Richard-Gr, R Varo, E López-Vare, A Sitoe, P Vitorino, J Bramugy, M Lanaspa, S Acácio, L Madrid, B Baro, KC Kain, Q Bassat
    PLOS global public health, 2023-02-23;3(2):e0001553.
    Species: Human
    Sample Types: Plasma
  12. Sugar-sweetened beverages exacerbate high-fat diet-induced inflammatory bowel disease by altering the gut microbiome: Sweetened beverages enhance fat-induced IBD
    Authors: WJ Shon, MH Jung, Y Kim, GH Kang, EY Choi, DM Shin
    The Journal of nutritional biochemistry, 2022-12-24;0(0):109254.
    Species: Human
    Sample Types: Serum
  13. NLRP3-Inflammasome Inhibition with IZD334 Does Not Reduce Cardiac Damage in a Pig Model of Myocardial Infarction
    Authors: MJM Silvis, EJ Demkes, L Timmers, F Arslan, SCA de Jager, JPG Sluijter, A Mosterd, DPV de Kleijn, L Bosch, GPJ van Hout
    Biomedicines, 2022-11-28;10(12):.
    Species: Porcine
    Sample Types: Plasma
  14. High levels of soluble RAGE are associated with a greater risk of mortality in COVID-19 patients treated with dexamethasone
    Authors: L Butcher, JC Zaldua, JA Carnicero, K Hawkins, J Whitley, R Mothukuri, PA Evans, K Morris, S Pillai, JD Erusalimsk
    Respiratory Research, 2022-11-05;23(1):303.
    Species: Human
    Sample Types: Serum
  15. Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria
    Authors: ML Sarangam, R Namazzi, D Datta, C Bond, CPB Vanderpool, RO Opoka, CC John, AL Conroy
    MBio, 2022-09-07;0(0):e0132522.
    Species: Human
    Sample Types: Serum
  16. Association between Leptin (G2548A) and Leptin Receptor (Q223R) Polymorphisms with Plasma Leptin, BMI, Stress, Sleep and Eating Patterns among the Multiethnic Young Malaysian Adult Population from a Healthcare University
    Authors: J Mohanraj, UJA D'Souza, SY Fong, IR Karkada, H Jaiprakash
    Oncogene, 2022-07-21;19(14):.
    Species: Human
    Sample Types: Plasma
  17. Impact of Zinc to Copper Ratio and Lipocalin 2 in Obese Patients Undergoing Sleeve Gastrectomy
    Authors: HM Demerdash, AA Sabry, OE Arida
    Oncogene, 2022-06-10;2022(0):9278531.
    Species: Human
    Sample Types: Serum
  18. Infant inflammation predicts childhood emotional and behavioral problems and partially mediates socioeconomic disadvantage
    Authors: C Pham, S Bekkering, M O'Hely, D Burgner, S Thomson, P Vuillermin, F Collier, W Marx, T Mansell, C Symeonides, PD Sly, M Lk Tang, R Saffery, AL Ponsonby
    Brain, Behavior, and Immunity, 2022-05-23;0(0):.
    Species: Human
    Sample Types: Serum
  19. Early life infection and proinflammatory, atherogenic metabolomic and lipidomic profiles in infancy: a population-based cohort study
    Authors: T Mansell, R Saffery, S Burugupall, AL Ponsonby, MLK Tang, M O'Hely, S Bekkering, AAT Smith, R Rowland, S Ranganatha, PD Sly, P Vuillermin, F Collier, P Meikle, D Burgner, Barwon Inf
    Elife, 2022-05-10;11(0):.
    Species: Human
    Sample Types: Plasma
  20. Efficacy of iron-folic acid treatment for reducing anemia prevalence and improving iron status in women of reproductive age: A one-year longitudinal study
    Authors: R Palika, T Dasi, S Ghosh, R Peter, DJ Parasannan, AS Pradhan, AV Kurpad, HS Sachdev, B Kulkarni, R Pullakhand
    Clinical Nutrition ESPEN, 2022-03-23;49(0):390-397.
    Species: Human
    Sample Types: Plasma
  21. The effect of transdermal gender-affirming hormone therapy on markers of inflammation and hemostasis
    Authors: MH Schutte, R Kleemann, NM Nota, CM Wiepjes, JM Snabel, G T'Sjoen, A Thijs, M den Heijer
    PLoS ONE, 2022-03-15;17(3):e0261312.
    Species: Human
    Sample Types: Plasma
  22. Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula
    Authors: S Hakki, EJ Robinson, MG Robson
    Scientific Reports, 2022-01-26;12(1):1427.
    Species: Human
    Sample Types: Plasma
  23. Clinical sign and biomarker-based algorithm to identify bacterial pneumonia among outpatients with lower respiratory tract infection in Tanzania
    Authors: SKL Hogendoorn, L Lhopitalli, M Richard-Gr, E Tenisch, Z Mbarack, J Samaka, T Mlaganile, A Mamin, B Genton, L Kaiser, V D'Acremont, KC Kain, N Boillat-Bl
    BMC infectious diseases, 2022-01-06;22(1):39.
    Species: Human
    Sample Types: Plasma
  24. Maternal inflammatory and omega-3 fatty acid pathways mediate the association between socioeconomic disadvantage and childhood cognition
    Authors: W Marx, S Thomson, M O'Hely, C Symeonides, F Collier, MLK Tang, A Loughman, D Burgner, R Saffery, C Pham, T Mansell, PD Sly, P Vuillermin, S Ranganatha, AL Ponsonby, Barwon Inf
    Brain, Behavior, and Immunity, 2021-12-08;100(0):211-218.
    Species: Human
    Sample Types: Serum
  25. Cytomegalovirus Immunity, Inflammation and Cognitive Abilities in the Elderly
    Authors: J Hesson, N Fudge, M Grant
    Viruses, 2021-11-21;13(11):.
    Species: Human
    Sample Types: Plasma
  26. Risky family climates presage increased cellular aging in young adulthood
    Authors: GH Brody, T Yu, E Chen, M Kobor, SRH Beach, MK Lei, A Barr, DT Lin, GE Miller
    Psychoneuroendocrinology, 2021-05-14;130(0):105256.
    Species: Human
    Sample Types: Serum
  27. Changes in liver steatosis in HIV-positive women are associated with the BMI, but not with biomarkers
    Authors: R Fernandez-, MW Plankey, D Ware, J Bordon
    Cytokine, 2021-05-12;0(0):155573.
    Species: Human
    Sample Types: Plasma
  28. Cross-Sectional Estimation of Endogenous Biomarker Associations with Prenatal Phenols, Phthalates, Metals, and Polycyclic Aromatic Hydrocarbons in Single-Pollutant and Mixtures Analysis Approaches
    Authors: MT Aung, Y Yu, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, B Mukherjee, JD Meeker
    Environmental health perspectives, 2021-03-24;129(3):37007.
    Species: Human
    Sample Types: Plasma
  29. Effect of Anti-Rheumatic Treatment on the Periodontal Condition of Rheumatoid Arthritis Patients
    Authors: MJ de Smit, J Westra, MD Posthumus, G Springer, AJ van Winkel, A Vissink, E Brouwer, M Bijl
    International Journal of Environmental Research and Public Health, 2021-03-04;18(5):.
    Species: Human
    Sample Types: Serum
  30. Association of circulating proprotein convertase subtilisin/kexin type 9 levels and the risk of incident type 2 diabetes in subjects with prediabetes: a population-based cohort study
    Authors: J Shi, W Zhang, Y Niu, N Lin, X Li, H Zhang, R Hu, G Ning, J Fan, L Qin, Q Su, Z Yang
    Cardiovascular Diabetology, 2020-12-10;19(1):209.
    Species: Human
    Sample Types:
  31. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020-11-19;0(0):.
    Species: Human
    Sample Types: Plasma
  32. Application of an analytical framework for multivariate mediation analysis of environmental data
    Authors: MT Aung, Y Song, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, JD Meeker, B Mukherjee
    Nat Commun, 2020-11-06;11(1):5624.
    Species: Human
    Sample Types: Plasma
  33. Circulating heat shock protein 27 as a novel marker of subclinical atherosclerosis in type 2 diabetes: a cross-sectional community-based study
    Authors: X Wang, J Shi, B Lu, W Zhang, Y Yang, J Wen, R Hu, Z Yang, X Wang
    BMC Cardiovasc Disord, 2020-04-25;20(1):198.
    Species: Human
    Sample Types: Serum
  34. Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients
    Authors: S Sindhu, S Kochumon, R Thomas, A Bennakhi, F Al-Mulla, R Ahmad
    Cells, 2020-03-16;9(3):.
    Species: Human
    Sample Types: Plasma
  35. Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children
    Authors: TN Chimponda, C Mushayi, DNM Osakunor, A Vengesai, E Enwono, S Amanfo, J Murray, C Tshuma, F Mutapi, T Mduluza
    BMC Infect. Dis., 2019-12-19;19(1):1071.
    Species: Human
    Sample Types: Serum
  36. Prediction and associations of preterm birth and its subtypes with eicosanoid enzymatic pathways and inflammatory markers
    Authors: MT Aung, Y Yu, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, B Mukherjee, JD Meeker
    Sci Rep, 2019-11-19;9(1):17049.
    Species: Human
    Sample Types: Plasma
  37. Increased Adipose Tissue Expression of Interferon Regulatory Factor (IRF)-5 in Obesity: Association with Metabolic Inflammation
    Authors: S Sindhu, R Thomas, S Kochumon, A Wilson, M Abu-Farha, A Bennakhi, F Al-Mulla, R Ahmad
    Cells, 2019-11-11;8(11):.
    Species: Human
    Sample Types: Plasma
  38. Average and time-specific maternal prenatal inflammatory biomarkers and the risk of labor epidural associated fever
    Authors: DY Arce, A Bellavia, DE Cantonwine, OJ Napoli, JD Meeker, T James-Todd, TF McElrath, LC Tsen
    PLoS ONE, 2019-11-05;14(11):e0222958.
    Species: Human
    Sample Types: Plasma
  39. Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection
    Authors: TT Tsai, TH Huang, NY Ho, YP Chen, CA Chen, CF Chen
    Sci Rep, 2019-10-30;9(1):15679.
    Species: Human
    Sample Types: Synovial Fluid
  40. Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study
    Authors: RE Elphinston, AM Weckman, CR McDonald, V Tran, K Zhong, M Madanitsa, L Kalilani-P, C Khairallah, SM Taylor, SR Meshnick, V Mwapasa, FO Ter Kuile, AL Conroy, KC Kain
    PLoS Med., 2019-10-01;16(10):e1002914.
    Species: Human
    Sample Types: Plasma
  41. Systemic inflammation is associated with malaria and preterm birth in women living with HIV on antiretrovirals and co-trimoxazole
    Authors: CR McDonald, AM Weckman, AL Conroy, P Olwoch, P Natureeba, MR Kamya, DV Havlir, G Dorsey, KC Kain
    Sci Rep, 2019-05-01;9(1):6758.
    Species: Human
    Sample Types: Plasma
  42. Association of antenatal depression with oxidative stress and impact on spontaneous preterm birth
    Authors: KK Venkatesh, JD Meeker, DE Cantonwine, TF McElrath, KK Ferguson
    J Perinatol, 2019-02-05;0(0):.
    Species: Human
    Sample Types: Plasma
  43. Effects of continuous moderate exercise with partial blood flow restriction during hemodialysis: A protocol for a randomized clinical trial
    Authors: RK Cardoso, AM Araujo, RB Orcy, M Bohlke, JP Oses, FB Del Vecchi, FC Barcellos, MC Gonzalez, AJ Rombaldi
    MethodsX, 2019-01-23;6(0):190-198.
    Species: Human
    Sample Types: Serum
  44. Plasma hepcidin is associated with future risk of venous thromboembolism.
    Authors: Ellingsen T, Lappegard J, Ueland T, Aukrust P, Braekkan S, Hansen J
    Blood Adv, 2018-06-12;2(11):1191-1197.
    Species: Human
    Sample Types: Plasma
  45. Relationship of the Adherence to a Mediterranean Diet and Its Main Components with CRP Levels in the Spanish Population
    Authors: C Lahoz, E Castillo, JM Mostaza, O de Dios, MA Salinero-F, T González-A, F García-Igl, E Estirado, F Laguna, V Sanchez, C Sabín, S López, V Cornejo, C de Burgos, C Garcés
    Nutrients, 2018-03-20;10(3):.
    Species: Human
    Sample Types: Plasma
  46. High serum levels of C-reactive protein (CRP) predict beneficial decrease of visceral fat in obese females after sleeve gastrectomy
    Authors: F Carbone, E Nulli Migl, A Bonaventur, A Vecchié, S De Vuono, MA Ricci, G Vaudo, M Boni, F Dallegri, F Montecucco, G Lupattelli
    Nutr Metab Cardiovasc Dis, 2018-02-02;0(0):.
    Species: Human
    Sample Types: Serum
  47. Effects of freezer storage time on levels of complement biomarkers
    Authors: AR Morgan, C O'Hagan, S Touchard, S Lovestone, B Paul Morga
    BMC Res Notes, 2017-11-06;10(1):559.
    Species: Human
    Sample Types: Plasma
  48. Monocyte/macrophage and T cell activation markers are not independently associated with MI risk in healthy individuals - results from the HUNT Study
    Authors: T Ueland, LE Laugsand, LJ Vatten, I Janszky, C Platou, AE Michelsen, JK Damås, P Aukrust, BO Åsvold
    Int. J. Cardiol., 2017-06-12;0(0):.
    Species: Human
    Sample Types: Serum
  49. Association of calf circumference with insulin resistance and non-alcohol fatty liver disease: the REACTION study
    Authors: W Zhang, Z Yang, Y Niu, X Li, L Zhu, S Lu, H Zhang, J Fan, G Ning, L Qin, Q Su
    BMC Endocr Disord, 2017-05-30;17(1):28.
    Species: Human
    Sample Types: Serum
  50. Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways
    Authors: A Burban, A Sharanek, R Hüe, M Gay, S Routier, A Guillouzo, C Guguen-Gui
    Sci Rep, 2017-05-12;7(1):1815.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. Experimental type 2 diabetes induction reduces serum vaspin, but not serum omentin, in Wistar rats
    Authors: CA Castro, KA da Silva, MM Buffo, KNZ Pinto, FO Duarte, KO Nonaka, FF Aníbal, ACGO Duarte
    Int J Exp Pathol, 2017-04-25;0(0):.
    Species: Human
    Sample Types: Serum
  52. The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis
    Authors: H Lavi, M Assayag, A Schwartz, N Arish, ZG Fridlender, N Berkman
    PLoS ONE, 2017-03-02;12(3):e0171945.
    Species: Human
    Sample Types: Plasma
  53. Improvement of cardiometabolic markers after fish oil intervention in young Mexican adults and the role of PPAR? L162V and PPAR?2 P12A
    Authors: A Binia, C Vargas-Mar, M Ancira-Mor, LM Gosoniu, I Montoliu, E Gámez-Vald, DC Soria-Cont, A Angeles-Qu, R Gonzalez-A, S Fernández, D Martínez-C, B Hernández-, M Ramírez-So, C Pérez-Orte, Y Rodríguez-, I Castan, I Rubio-Alia, F Vadillo-Or, R Márquez-Ve, R Bojalil, JC López-Alva, P Valet, M Kussmann, I Silva-Zole, ME Tejero
    J. Nutr. Biochem., 2017-02-10;43(0):98-106.
    Species: Human
    Sample Types: Serum
  54. Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects
    Sci Rep, 2016-11-25;6(0):37886.
    Species: Human
    Sample Types: Serum
  55. Plasma selenium levels and nonalcoholic fatty liver disease in Chinese adults: a cross-sectional analysis
    Sci Rep, 2016-11-17;6(0):37288.
    Species: Human
    Sample Types: Serum
  56. Intraplaque Expression of C-Reactive Protein Predicts Cardiovascular Events in Patients with Severe Atherosclerotic Carotid Artery Stenosis
    Authors: Aldo Bonaventur
    Mediators Inflamm, 2016-09-21;2016(0):9153673.
    Species: Human
    Sample Types: Serum
  57. Silk-based blood stabilization for diagnostics
    Proc Natl Acad Sci USA, 2016-05-09;113(21):5892-7.
    Species: Human
    Sample Types: Plasma
  58. Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study.
    Authors: Erdman L, D'Acremont V, Hayford K, Rajwans N, Kilowoko M, Kyungu E, Hongoa P, Alamo L, Streiner D, Genton B, Kain K
    PLoS ONE, 2015-09-14;10(9):e0137592.
    Species: Human
    Sample Types: Plasma
  59. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015-08-06;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  60. The effect of smoking on DNA methylation of peripheral blood mononuclear cells from African American women.
    Authors: Dogan, Meeshant, Shields, Bridget, Cutrona, Carolyn, Gao, Long, Gibbons, Frederic, Simons, Ronald, Monick, Martha, Brody, Gene H, Tan, Kai, Beach, Steven R, Philibert, Robert A
    BMC Genomics, 2014-02-22;15(0):151.
    Species: Human
    Sample Types: Serum
  61. Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis.
    Authors: Quon B, Ngan D, Wilcox P, Man S, Sin D
    PLoS ONE, 2014-02-20;9(2):e89341.
    Species: Human
    Sample Types: Plasma
  62. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014-01-20;14(0):35.
    Species: Human
    Sample Types: Serum
  63. Differences in insulin sensitivity, lipid metabolism and inflammation between young adult Pakistani and Norwegian patients with type 2 diabetes: a cross sectional study.
    Authors: Wium C, Aasheim E, Ueland T, Michelsen A, Thorsby P, Larsen I, Torjesen P, Aukrust P, Birkeland K
    BMC Endocr Disord, 2013-10-22;13(0):49.
    Species: Human
    Sample Types: Plasma
  64. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011-12-12;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  65. Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
    Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
    PLoS ONE, 2011-02-25;6(2):e17440.
    Species: Human
    Sample Types: Plasma
  66. Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers.
    Authors: Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF
    Am. J. Respir. Crit. Care Med., 2009-03-26;179(12):1107-14.
    Species: Human
    Sample Types: BALF

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Reviews for Human C-Reactive Protein/CRP DuoSet ELISA

Average Rating: 5 (Based on 6 Reviews)

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Human C-Reactive Protein/CRP DuoSet ELISA
By Carmen Martin-Ruiz on 11/12/2020
Sample Tested: EDTA Plasma

Very good results, standard curve with good range and sensitivity, EDTA plasma samples diluted 1:4000!

Human C-Reactive Protein/CRP DuoSet ELISA DY1707
Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 03/30/2019
Sample Tested: Purified Standard
Human C-Reactive Protein/CRP DuoSet ELISA DY1707
Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 10/09/2018
Sample Tested: Purified Standard,Urine
Human C-Reactive Protein/CRP DuoSet ELISA DY1707
Human C-Reactive Protein/CRP DuoSet ELISA
By Anonymous on 09/11/2018
Sample Tested: Purified Standard
Human C-Reactive Protein/CRP DuoSet ELISA DY1707
Human C-Reactive Protein/CRP DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma
Human C-Reactive Protein/CRP DuoSet ELISA
By BOCHATON Thomas on 11/21/2017
Sample Tested: Serum

We used human serum at a dilution of 1:50000. The result was very good.

Human C-Reactive Protein/CRP DuoSet ELISA DY1707