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Human CCL17/TARC DuoSet ELISA

Catalog #: DY364
22 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY364
DY364-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CCL17 / TARC ELISA Standard Curve
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Product Details
Procedure
Citations (22)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CCL17/TARC DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL17/TARC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CCL17 / TARC ELISA Standard Curve View Larger

Human CCL17 / TARC ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL17/TARC

CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.

Entrez Gene IDs:
6361 (Human); 20295 (Mouse)
Alternate Names:
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CCL17/TARC DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The IL-4/13-induced production of M2 chemokines by human lung macrophages is enhanced by adenosine and PGE2
    Authors: Brollo, M;Salvator, H;Grassin-Delyle, S;Glorion, M;Descamps, D;Buenestado, A;Naline, E;Tenor, H;Tiotiu, A;Devillier, P;
    International immunopharmacology
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Bronchoalveolar cytokine profile differentiates Pulmonary Langerhans cell histiocytosis patients from other smoking-related interstitial lung diseases
    Authors: Barril, S;Acebo, P;Millan-Billi, P;Luque, A;Sibila, O;Tarín, C;Tazi, A;Castillo, D;Hortelano, S;
    Respiratory research
    Species: Human
    Sample Types: BALF
  3. Human interleukin-12? and EBI3 are cytokines with anti-inflammatory functions
    Authors: Hildenbrand, K;Bohnacker, S;Menon, PR;Kerle, A;Prodjinotho, UF;Hartung, F;Strasser, PC;Catici, DAM;Rührnö beta l, F;Haslbeck, M;Schumann, K;Müller, SI;da Costa, CP;Esser-von Bieren, J;Feige, MJ;
    Science advances
    Species: Human
    Sample Types: Cell Culture Supernates
  4. In vitro assessment of the anti-inflammatory and skin-moisturizing effects of Filipendula palmata (Pall.) Maxim. On human keratinocytes and identification of its bioactive phytochemicals
    Authors: XJ Mi, JK Kim, S Lee, SK Moon, YJ Kim, H Kim
    Journal of ethnopharmacology, 2022-07-07;296(0):115523.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis
    Authors: W Francuzik, K Pažur, M Dalke, S Dölle-Bier, M Babina, M Worm
    JCI Insight, 2022-04-08;0(0):.
    Species: Human
    Sample Types: Serum
  6. Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up
    Authors: B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay
    PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Gomisin M2 alleviates psoriasis?like skin inflammation by inhibiting inflammatory signaling pathways
    Authors: N Kim, S Lee, J Kang, TK Kwon, D Khang, SH Kim
    Molecular Medicine Reports, 2021-10-19;24(6):.
    Species: Human
    Sample Types: Serum
  8. Skullcapflavone II Suppresses TNF-alpha/IFN-gamma-Induced TARC, MDC, and CTSS Production in HaCaT Cells
    Authors: H Lee, DH Lee, JH Oh, JH Chung
    International Journal of Molecular Sciences, 2021-06-16;22(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  10. Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism
    Authors: M Mazzoni, G Mauro, M Erreni, P Romeo, E Minna, MG Vizioli, C Belgiovine, MG Rizzetti, S Pagliardin, R Avigni, MC Anania, P Allavena, MG Borrello, A Greco
    J. Exp. Clin. Cancer Res., 2019-05-21;38(1):208.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Rhododendron album Blume extract inhibits TNF-?/IFN-?-induced chemokine production via blockade of NF-?B and JAK/STAT activation in human epidermal keratinocytes
    Authors: JW Park, HS Lee, Y Lim, JH Paik, OK Kwon, JH Kim, I Paryanto, P Yunianto, S Choi, SR Oh, KS Ahn
    Int. J. Mol. Med., 2018-03-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells
    Authors: L Strasser, HH Dang, H Schwarz, C Asam, F Ferreira, J Horejs-Hoe, CG Huber
    Sci Rep, 2017-11-30;7(1):16638.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice
    PLoS ONE, 2016-12-09;11(12):e0167687.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015-02-24;10(2):e0116560.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015-01-15;10(1):e0116775.
    Species: Human
    Sample Types: BALF
  16. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014-06-09;5(0):4092.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Marked and persistent eosinophilia in the absence of clinical manifestations.
    Authors: Chen Y, Khoury P, Ware J, Holland-Thomas N, Stoddard J, Gurprasad S, Waldner A, Klion A
    J Allergy Clin Immunol, 2013-08-26;133(4):1195-202.
    Species: Human
    Sample Types: Serum
  18. Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae.
    Authors: Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P
    J. Immunol., 2011-03-02;186(7):3966-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Anemia in Hodgkin's lymphoma: the role of interleukin-6 and hepcidin.
    Authors: Hohaus S, Massini G, Giachelia M, Vannata B, Bozzoli V, Cuccaro A, D'Alo' F, Larocca LM, Raymakers RA, Swinkels DW, Voso MT, Leone G
    J. Clin. Oncol., 2010-04-20;28(15):2538-43.
    Species: Human
    Sample Types: Plasma
  20. Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
    Authors: Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
    Thorax, 2008-02-14;63(9):810-6.
    Species: Human
    Sample Types: Serum
  21. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Respiratory syncytial virus synergizes with Th2 cytokines to induce optimal levels of TARC/CCL17.
    Authors: Monick MM, Powers LS, Hassan I, Groskreutz D, Yarovinsky TO, Barrett CW, Castilow EM, Tifrea D, Varga SM, Hunninghake GW
    J. Immunol., 2007-08-01;179(3):1648-58.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Human CCL17/TARC DuoSet ELISA

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Human CCL17/TARC DuoSet ELISA
By Anonymous on 12/14/2020
Sample Tested: Cell culture supernatant
Human CCL17/TARC DuoSet ELISA DY364
Human CCL17/TARC DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma

We used this kit for the quantification of CCL17 in human serum and plasma. Works very well and well-described protocol.

Human CCL17/TARC DuoSet ELISA DY364