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Human Periostin/OSF-2 DuoSet ELISA

Catalog #: DY3548B
30 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3548B
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Periostin / OSF-2 ELISA Standard Curve
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Product Details
Procedure
Citations (30)
FAQs
Supplemental Products
Reviews (3)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Periostin/OSF-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Periostin/OSF-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human Periostin / OSF-2 ELISA Standard Curve View Larger

Human Periostin / OSF-2 ELISA Standard Curve

N/A View Larger

Human Periostin/OSF-2 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Periostin/OSF-2

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation. It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth. It is upregulated in many carcinomas. Periostin binds to multiple Integrins and enhances cell adhesion and cell migration. It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis. It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis. Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis. Periostin plays an important role in heart valve development and tissue healing after myocardial infarction. In asthma, it is upregulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production.

Long Name:
Osteoblast Specific Factor 2
Entrez Gene IDs:
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names:
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human Periostin/OSF-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Influence of diabetes mellitus on the pathological profile of aortic stenosis: a sex-based approach
    Authors: Martín-Núñez, E;Goñi-Olóriz, M;Matilla, L;Garaikoetxea, M;Mourino-Alvarez, L;Navarro, A;Fernández-Celis, A;Tamayo, I;Gainza, A;Álvarez, V;Sádaba, R;Barderas, MG;Jover, E;López-Andrés, N;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  2. Periostin deficiency attenuates kidney fibrosis in diabetic nephropathy by improving pancreatic ?-cell dysfunction and reducing kidney EMT
    Authors: Cho, A;Jin, W;Lee, J;Shin, N;Lee, MS;Li, L;Yang, SH;Park, KS;Yang, CW;Kim, DK;Oh, YK;Lim, CS;Lee, JP;
    Scientific reports
    Species: Human
    Sample Types: Urine
  3. Simple, low-cost, and well-performing method, the outgrowth technique, for the isolation of cells from nasal polyps
    Authors: Kim, J;Hegener, K;Hagedorn, C;Weidinger, D;Jamal Jameel, K;Seuthe, IMC;Eichhorn, S;Kreppel, F;Park, JJ;Knobloch, J;
    BMC molecular and cell biology
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Abnormal basement membrane results in increased keratinocyte-derived periostin expression in psoriasis similar to wound healing
    Authors: Flink, LB;Ghaffarinia, A;Papp, BT;Varga, Á;Vigh, AI;Vidács, DL;Kui, R;Kemény, L;Bata-Csörg?, Z;Bozó, R;
    Scientific reports
    Species: Human
    Sample Types: Serum
  5. CTHRC1 Induces Pancreatic Stellate Cells (PSCs) into Myofibroblast-like Cancer-Associated Fibroblasts (myCAFs)
    Authors: Kang, MK;Jiang, F;Kim, YJ;Ryu, K;Masamune, A;Hamada, S;Park, YY;Koh, SS;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Long-Term Cryopreservation of Nasal Polyp Tissue in a Biobank for the Isolation and Culture of Primary Epithelial Cells
    Authors: J Kim, K Hegener, C Hagedorn, K Jamal Jame, D Weidinger, IMC Seuthe, S Eichhorn, F Kreppel, J Knobloch, JJ Park
    International Journal of Molecular Sciences, 2023-03-28;24(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Nasal TSLP and periostin in infants with severe bronchiolitis and risk of asthma at 4�years of age
    Authors: ML Garcia-Gar, B Sastre, M Arroyas, M Beato, P Alonso, JM Rodrigo-Mu, V Del Pozo, I Casas, C Calvo
    Respiratory Research, 2023-01-24;24(1):26.
    Species: Human
    Sample Types: Nasopharyngeal aspirate
  8. Periostin Augments Vascular Smooth Muscle Cell Calcification via beta-Catenin Signaling
    Authors: I Alesutan, LA Henze, B Boehme, TTD Luong, D Zickler, B Pieske, KU Eckardt, A Pasch, J Voelkl
    Biomolecules, 2022-08-21;12(8):.
    Species: Human
    Sample Types: Serum
  9. Soluble POSTN is a novel biomarker complementing CA153 and CEA for breast cancer diagnosis and metastasis prediction
    Authors: L Jia, G Li, N Ma, A Zhang, Y Zhou, L Ren, D Dong
    BMC Cancer, 2022-07-12;22(1):760.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Serum Periostin Level and Genetic Polymorphisms Are Associated with Vertebral Fracture in Chinese Postmenopausal Women
    Authors: YM Guo, JH Cheng, H Zhang, JW He, H Yue, WW Hu, JM Gu, YQ Hu, WZ Fu, C Wang, ZL Zhang
    Genes, 2022-02-27;13(3):.
    Species: Human
    Sample Types: Serum
  11. Application of QPLEXTM biomarkers in cognitively normal individuals across a broad age range and diverse regions with cerebral amyloid deposition
    Authors: D Lee, JC Park, KS Jung, J Kim, JS Jang, S Kwon, MS Byun, D Yi, G Byeon, G Jung, YK Kim, DY Lee, SH Han, I Mook-Jung
    Experimental & Molecular Medicine, 2022-01-20;0(0):.
    Species: Human
    Sample Types: Plasma
  12. Involvement of the extracellular matrix proteins periostin and tenascin C in nasal polyp remodeling by regulating the expression of MMPs
    Authors: K Du, M Wang, N Zhang, P Yu, P Wang, Y Li, X Wang, L Zhang, C Bachert
    Clinical and translational allergy, 2021-09-06;11(7):e12059.
    Species: Human
    Sample Types: Tissue Homogenates
  13. Pathological Roles and Clinical Usefulness of Periostin in Type 2 Inflammation and Pulmonary Fibrosis
    Authors: J Ono, M Takai, A Kamei, Y Azuma, K Izuhara
    Biomolecules, 2021-07-22;11(8):.
    Species: Human
    Sample Types: Serum
  14. Expression profile of the matricellular protein periostin in paediatric inflammatory bowel disease
    Authors: T Coelho, E Sonnenberg, Y Gao, E Mossotto, A Khojanazar, A Griffin, S Mukanova, A Ashimkhano, R Haggarty, A Borissenko, JJ Ashton, IS Stafford, A Batra, NA Afzal, MP Stanton, B Vadgama, K Adrisova, RM Beattie, AP Williams, S Ennis, D Riethmache
    Scientific Reports, 2021-03-18;11(1):6194.
    Species: Human
    Sample Types: Plasma
  15. The potential pathways underlying the association of propyl-paraben exposure with aeroallergen sensitization and EASI score using metabolomics analysis
    Authors: Y Lee, E Lee, DK Yon, HM Jee, HS Baek, SW Lee, JY Cho, MY Han
    Scientific Reports, 2021-02-12;11(1):3772.
    Species: Human
    Sample Types: Serum
  16. iPSC-Derived Hereditary Breast Cancer Model Reveals the BRCA1-Deleted Tumor Niche as a New Culprit in Disease Progression
    Authors: L Portier, C Desterke, D Chaker, N Oudrhiri, A Asgarova, F Dkhissi, AG Turhan, A Bennaceur-, F Griscelli
    International Journal of Molecular Sciences, 2021-01-27;22(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
    Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
    Sci Rep, 2020-10-15;10(1):17444.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Efficacy of endoscopic sinus surgery for eosinophilic chronic rhinosinusitis with asthma
    Authors: N Ohta, Y Suzuki, H Ikeda, N Noguchi, R Kakuta, T Suzuki, R Ikeda, M Yamazaki, Y Saito, Y Kusano, Y Ishida, F Shoji, H Yoshioka, J Ono, T Takahashi, Y Nakamura, I Ohno, K Izuhara
    Allergol Int, 2019-09-05;0(0):.
    Species: Human
    Sample Types: Serum
  19. A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
    Authors: J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang
    Clin Proteomics, 2019-03-05;16(0):10.
  20. Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells
    Authors: Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang
    Cell Rep, 2019-02-05;26(6):1533-1543.e4.
    Species: Human
    Sample Types: Plasma
  21. Elevated serum periostin level in patients with chronic cough and airway hyperresponsiveness
    Authors: Y Kono, M To, R Tsuzuki, K Haruki, Y To
    Respir Investig, 2018-12-12;57(2):122-125.
    Species: Human
    Sample Types: Serum
  22. IL-13 induces periostin and eotaxin expression in human primary alveolar epithelial cells: Comparison with paired airway epithelial cells
    Authors: Y Ito, R Al Mubarak, N Roberts, K Correll, W Janssen, J Finigan, R Mishra, HW Chu
    PLoS ONE, 2018-04-19;13(4):e0196256.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Mixed Th2 and non-Th2 inflammatory pattern in the asthma-COPD overlap: a network approach
    Authors: LP de Llano, BG Cosío, A Iglesias, N de Las Cue, JJ Soler-Cata, JL Izquierdo, JL López-Camp, C Calero, V Plaza, M Miravitlle, A Torrego, E Martinez-M, JB Soriano, AL Viña, I Bobolea
    Int J Chron Obstruct Pulmon Dis, 2018-02-12;13(0):591-601.
    Species: Human
    Sample Types: Serum
  24. Asthma-COPD overlap is not a homogeneous disorder: further supporting data
    Authors: L Pérez-de-L, BG Cosio
    Respir. Res., 2017-11-02;18(1):183.
    Species: Human
    Sample Types: Serum
  25. Cigarette smoke differentially affects IL-13-induced gene expression in human airway epithelial cells
    Authors: TCJ Mertens, AM van der Do, LE Kistemaker, DK Ninaber, C Taube, PS Hiemstra
    Physiol Rep, 2017-07-11;5(13):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Activated hepatic stellate cells secrete periostin to induce stem cell-like phenotype of residual hepatocellular carcinoma cells after heat treatment
    Authors: R Zhang, RR Yao, JH Li, G Dong, M Ma, QD Zheng, DM Gao, JF Cui, ZG Ren, RX Chen
    Sci Rep, 2017-05-19;7(1):2164.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization
    Authors: T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi
    Mol Ther Nucleic Acids, 2017-02-09;6(0):279-289.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects
    Sci Rep, 2016-11-25;6(0):37886.
    Species: Human
    Sample Types: Serum
  29. Urinary Periostin Excretion Predicts Renal Outcome in IgA Nephropathy
    Authors: Chun Soo Lim
    Am. J. Nephrol., 2016-11-01;44(6):481-492.
    Species: Human
    Sample Types: Urine
  30. Polycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.
    Authors: Ple C, Fan Y, Ait Yahia S, Vorng H, Everaere L, Chenivesse C, Balsamelli J, Azzaoui I, de Nadai P, Wallaert B, Lazennec G, Tsicopoulos A
    PLoS ONE, 2015-04-10;10(4):e0122372.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human Periostin/OSF-2 DuoSet ELISA

Average Rating: 4.7 (Based on 3 Reviews)

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Human Periostin/OSF-2 DuoSet ELISA
By Nadezhda Glezeva on 11/22/2021
Sample Tested: Serum and Plasma

sample dilution used 1:10

Human Periostin/OSF-2 DuoSet ELISA DY3548B
Human Periostin/OSF-2 DuoSet ELISA
By Anonymous on 09/09/2021
Sample Tested: stem cell condition medium
Human Periostin/OSF-2 DuoSet ELISA DY3548B
Human Periostin/OSF-2 DuoSet ELISA
By Anonymous on 07/26/2018
Sample Tested: EDTA Plasma
Human Periostin/OSF-2 DuoSet ELISA DY3548B